• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Investigation of the interplay among enzymes and transporters involved in sulfur metabolism in plants

Research Project

Project/Area Number 23770036
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionChiba University

Principal Investigator

YOSHIMOTO Naoko  千葉大学, 薬学研究科(研究院), 助教 (10415333)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords植物 / 代謝 / 硫黄 / 輸送 / 相互作用 / 分子間相互作用
Research Abstract

Plants are able to take up sulfate from the soil solution and convert it into sulfur-containing amino acids, thiols, and sulfur-containing secondary metabolites. In this project, we determined physical interactions among transporters and enzymes that are involved in sulfur metabolism. Transgenic plants co-overexpressing two interacting enzymes essential for the synthesis of amino acid cysteine showed enhanced production of thiol compounds under cadmium-stressed conditions, and exhibited tolerance to cadmium. We also identified enzymes required for the biosynthesis of sulfur-containing secondary metabolites.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (24 results)

All 2014 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (18 results) Book (2 results) Remarks (1 results)

  • [Journal Article] An improved tolerance to cadmium by overexpression of two genes for cysteine synthesis in tobacco2014

    • Author(s)
      Michimi Nakamura, Tomoko Ochiai, Masaaki Noji, Yasumitsu Ogura, Kazuo T. Suzuki, Naoko Yoshimoto, Mami Yamazaki, Kazuki Saito
    • Journal Title

      Plant Biotechnology

      Volume: 31 Issue: 2 Pages: 141-147

    • DOI

      10.5511/plantbiotechnology.14.0130a

    • NAID

      130003391375

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evolutionary relationships and functional diversity of plant sulfate transporters2012

    • Author(s)
      Hideki Takahashi, Peter Buchner, Naoko Yoshimoto, Malcolm J. Hawkesford, Shin-Han Shiu
    • Journal Title

      Frontiers Plant Sci

      Volume: Vol 2, Article 119 Pages: 1-9

    • DOI

      10.3389/fpls.2011.00119

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interplay of SLIM1 and miR395 in the regulation of sulfate assimilation in Arabiopsis2011

    • Author(s)
      Cintia G. Kawashima, Colette A. Matthewman, Siqi Huang, Bok-Rye Lee, Naoko Yoshimoto, Anna Koprivova, Ignacio Rubio-Somoza, Marco Todesco, Tina Rathjen, Kazuki Saito, Hideki Takahashi, Tamas Dalmay, Stanislav Kopriva
    • Journal Title

      Plant J.

      Volume: 66 Issue: 5 Pages: 863-876

    • DOI

      10.1111/j.1365-313x.2011.04547.x

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] ニンニクの含硫二次代謝物アリインの生合成に関わる酵素遺伝子群の機能解析2014

    • Author(s)
      吉本尚子,矢部綾美,小沼美沙都,杉野由佳,中林亮,上山正恵,鎌田庸宏,今井真介,角愼一郎,恒吉唯充,斉藤和季
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      (熊本)
    • Related Report
      2013 Final Research Report
  • [Presentation] ニンニクの含硫二次代謝物アリインの生合成に関与する酵素遺伝子群の同定と機能解析2014

    • Author(s)
      吉本尚子,矢部綾美,小沼美沙都,杉野由佳,中林亮,上山正恵,鎌田庸宏,今井真介,角愼一郎,恒吉唯充,斉藤和季
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      (富山)
    • Related Report
      2013 Final Research Report
  • [Presentation] ニンニクの含硫二次代謝物アリインの生合成に関与する酵素遺伝子群の同定と機能解析2014

    • Author(s)
      吉本尚子、矢部綾美、小沼美沙都、杉野由佳、中林亮、上山正恵、鎌田庸宏、今井真介、角愼一郎、恒吉唯充、斉藤和季
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学(富山県富山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ニンニクの含硫二次代謝物アリインの生合成に関わる酵素遺伝子群の機能解析2014

    • Author(s)
      吉本尚子、矢部綾美、小沼美沙都、杉野由佳、中林亮、上山正恵、鎌田庸宏、今井真介、角愼一郎、恒吉唯充、斉藤和季
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本大学(熊本県熊本市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ニンニクのアリイン生合成に関与するフラビン含有モノオキシゲナーゼの機能解析2013

    • Author(s)
      小沼美沙都,吉本尚子,水野新也,上山正恵,鎌田庸宏,今井真介,角愼一郎,恒吉唯充,斉藤和季
    • Organizer
      第31回日本植物細胞分子生物学会
    • Place of Presentation
      (北海道)
    • Related Report
      2013 Final Research Report
  • [Presentation] ニンニクのアリイン生合成に関わるγ-グルタミルトランスペプチダーゼ遺伝子の単離と解析2013

    • Author(s)
      矢部綾美,吉本尚子,杉野由佳、村上聡一郎,Niti Sai-ngam,角愼一郎,恒吉唯充,斉藤和季
    • Organizer
      日本生薬学会第60回年会
    • Place of Presentation
      (北海道)
    • Related Report
      2013 Final Research Report
  • [Presentation] ニンニクのアリイン生合成に関わるS-酸化酵素遺伝子の単離と解析2013

    • Author(s)
      小沼美沙都,吉本尚子,水野新也,上山正恵,鎌田庸宏,今井真介,角愼一郎,恒吉唯充,斉藤和季
    • Organizer
      日本生薬学会第60回年会
    • Place of Presentation
      (北海道)
    • Related Report
      2013 Final Research Report
  • [Presentation] ニンニクのアリイン生合成に関与するフラビン含有モノオキシゲナーゼ遺伝子の単離と機能解析2013

    • Author(s)
      吉本尚子,水野新也,小沼美沙都,上山正恵,鎌田庸宏,今井真介,角愼一郎,恒吉唯充,斉藤和季
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      (横浜)
    • Related Report
      2013 Final Research Report
  • [Presentation] ニンニクのS-酸化化合物生合成に関与する新規フラビン含有モノオキシゲナーゼ遺伝子の単離と解析2013

    • Author(s)
      吉本尚子,水野新也,小沼美沙都,上山正恵,鎌田庸宏,今井真介,角愼一郎,恒吉唯充,斉藤和季
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      (岡山)
    • Related Report
      2013 Final Research Report
  • [Presentation] ニンニクのアリイン生合成に関わるS酸化酵素遺伝子の単離と解析2013

    • Author(s)
      小沼美沙都、吉本尚子、水野新也、上山正恵、鎌田庸宏、今井真介、角愼一郎、恒吉唯充、斉藤和季
    • Organizer
      日本生薬学会第60回年会
    • Place of Presentation
      北海道医療大学(北海道当別町)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ニンニクのアリイン生合成に関わるgamma-グルタミルトランスペプチダーゼ遺伝子の単離と解析2013

    • Author(s)
      矢部綾美、吉本尚子、杉野由佳、村上聡一郎、Niti Sai-ngam、角愼一郎、恒吉唯充、斉藤和季
    • Organizer
      日本生薬学会第60回年会
    • Place of Presentation
      北海道医療大学(北海道当別町)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ニンニクのアリイン生合成に関与するフラビン含有モノオキシゲナーゼの機能解析2013

    • Author(s)
      小沼美沙都、吉本尚子、水野新也、上山正恵、鎌田庸宏、今井真介、角愼一郎、恒吉唯充、斉藤和季
    • Organizer
      第31回日本植物細胞分子生物学会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ニンニクのS-酸化化合物生合成に関与する新規フラビン含有モノオキシゲナーゼ遺伝子の単離と解析2013

    • Author(s)
      吉本尚子
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2012 Research-status Report
  • [Presentation] ニンニクのアリイン生合成に関与するフラビン含有モノオキシゲナーゼ遺伝子の単離と機能解析2013

    • Author(s)
      吉本尚子
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] シロイヌナズナの高親和型硫酸イオントランスポーター間相互作用による硫酸イオン輸送活性の調節2012

    • Author(s)
      吉本尚子,斉藤和季,高橋秀樹
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      (京都)
    • Related Report
      2013 Final Research Report
  • [Presentation] シロイヌナズナの高親和型硫酸イオントランスポーター間相互作用による硫酸イオン輸送活性の調節2012

    • Author(s)
      吉本尚子
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Related Report
      2011 Research-status Report
  • [Presentation] Regulation of high-affinity sulfate transporters in Arabidopsis by sulfur nutrition2011

    • Author(s)
      Naoko Yoshimoto, Eri Inoue, Akiko Watanabe-Takahashi, Kazuki Saito, Hideki Takahashi
    • Organizer
      Japan-Korea Joint Seminar on Plant Biotechnology for the Next Generation
    • Place of Presentation
      (成田)
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of high-affinity sulfate transporters in Arabidopsis by sulfur nutrition.2011

    • Author(s)
      Naoko Yoshimoto
    • Organizer
      Japan-Korea Joint Seminar on "Plant Biotechnology for the Next Generation"(招待講演)
    • Place of Presentation
      成田
    • Related Report
      2011 Research-status Report
  • [Book] Moleculat and cellular regulation of sulfate transport and assimilation. in"Sulfur Metabolism in Plants. Mechanisms and Applications to Food Security and Responses to Climate Change"eds. L. De Kok, M. Tausz, M.J. Hawkesford, R. Hoefgen, M.T. Mcmanus, R.M. Norton, H. Rennenberg, K. Saito, E. Schnug, L. Tabe2012

    • Author(s)
      Naoko Yoshimoto, Kazuki Saito
    • Publisher
      Springer(DOI :10.1007/978-94-007-4450-9)
    • Related Report
      2013 Final Research Report
  • [Book] Partitioning of sulfur between primary and secondary metabolism. in"Sulfur Metabolism in Plants. Mechanisms and Applications to Food Security and Responses to Climate Change"eds. L. De Kok, M. Tausz, M.J. Hawkesford, R. Hoefgen, M.T. Mcmanus, R.M. Norton, H. Rennenberg, K. Saito, E. Schnug, L. Tabe2012

    • Author(s)
      Sarah G. Mugford, Colette Matthewman, Bok-Rye Lee, Ruslan Yatusevich, Naoko Yoshimoto, Maukus Wirtz, Lionel Hill, Ruediger Hell, Hideki Takahashi, Kazuki Saito, Tamara Gigolashvili, Stanislav Kopriva
    • Publisher
      Springer (DOI :10.1007/978-94-007-4450-9)
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.p.chiba-u.ac.jp/lab/idenshi/index.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi