Elucidation of mechanisms of plant innate immunity by R protein

• Project/Area Number: 23770044
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: KAWANO Yoji 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (00406175)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: イネ / 抵抗性タンパク質 / OsRac1 / 耐病性 / 植物免疫

Research Abstract

R protein Pit acts as an intracellular receptors for sensing the blast fungus. In this project, we found that Pit activates small GTPase OsRac1 through a GDP/GTP exchange factor OsSPIKE1. Pit-dependent OsRac1 activation via OsSPIKE1 playsimportant role in Pit-mediated disease resistance. Palmitoylation of Pit plays a critical role in activation of small GTPase OsRac1 on the plasma membrane. In addition, we identified the novel interactors of Pit.

Research Products

• [Journal Article] An OsCEBiP / OsCERK1-OsRacGEF - OsRac1 module is an essential early component of chitin-induced rice immunity (2013)
  • Author(s): Akamatsu, A., Wong, H.L., Fujiwara, M., Okuda, J., Nishide, K., Uno, K., Imai, K., Umemura, K., Kawasaki, T ., Kawano, Y ., and Shimamoto, K.
  • Journal Title: Cell Host Microbe Volume: 13 Issue: 4 Pages: 465-476
  • DOI: 10.1016/j.chom.2013.03.007
  • Peer Reviewed
• [Journal Article] An OsCEBiP/OsCERK1-OsRacGEF1-OsRac1 Module Is an Essential Early Component of Chitin-Induced Rice Immunity (2013)
  • Author(s): Akamtsu A
  • Journal Title: Cell Host Microbe Volume: 4
  • Peer Reviewed
• [Journal Article] The bHLH Rac Immunityl (RAID) Is Activated by OsRacl via OsMPK3 and OsMAPK6 in Rice Immunity (2012)
  • Author(s): SH Kim, T., 他6名, Y.Kawano, 他1名, K.Shimamoto
  • Journal Title: Plant Cell Physiol Volume: 53 Issue: 4 Pages: 740-54
  • DOI: 10.1093/pcp/pcs033
  • Peer Reviewed
• [Journal Article] OsRap2.6 transcription factor contributes to rice innate immunity through its interaction with Receptor for Activated Kinase-C 1 (RACK1) (2012)
  • Author(s): Wamaitha MJ, Yamamoto R, Wong HL, Kawasaki T, Kawano Y, Shimamoto K
  • Journal Title: Rice Volume: 5 Pages: 35-35
  • URL: http://link.springer.com/article/10.1186%2F1939-8433-5-35
  • Peer Reviewed
• [Journal Article] Dynamic Regulation of Myosin Light Chain Phosphorylation by Rho-kinase (2012)
  • Author(s): Takako Kaneko-Kawano, Fugo Takasu, Honda Naoki, Yuichi Sakumura, Shin Ishii, Takahiro Ueba, Akinori Eiyama, Aiko Okada, Yoji Kawano, Kenji Suzuki
  • Journal Title: PLoSONE Volume: 7(6) Issue: 6 Pages: 1-10
  • DOI: 10.1371/journal.pone.0039269
  • Peer Reviewed
• [Journal Article] Interaction of α-taxilin localized on intracellular components with the microtubule cytoskeleton (2012)
  • Author(s): Horii Y, Nogami S, Kawano Y, Kaneko-Kawano T, Ohtomo N, Tomiya T, Shirataki H
  • Journal Title: Cell Struct Funct Volume: 37 Pages: 111-26
  • NAID: 40019582034
  • URL: https://www.jstage.jst.go.jp/article/csf/37/2/37_12002/_article
  • Peer Reviewed
• [Journal Article] The bHLH Rac Immunity1 (RAI1) Is Activated by OsRac1 via OsMAPK3 and OsMAPK6 in Rice Immunity (2012)
  • Author(s): Kim SH
  • Journal Title: Plant Cell Physiol. Volume: 4 Pages: 40-54
  • Peer Reviewed
• [Journal Article] Interaction of alpha-taxilin localized on intracellular components with the microtubule cytoskeleton (2012)
  • Author(s): Horii Y
  • Journal Title: Cell Struct Funct Volume: 2 Pages: 111-26
  • Peer Reviewed
• [Journal Article] The bHLH Rac Immunity1 (RAI1) Is Activ (2012)
  • Author(s): SH Kim, T. 他６名, Y. Kawano,他1名, and K. Shimamoto
  • Journal Title: Plant and Cell Physiology Volume: 53 Pages: 740-54
  • Peer Reviewed
• [Presentation] Elucidation of mechanisms of small GTPase OsRac1 activation by R protein Pit through OsSPIKE1 (2013)
  • Author(s): Yoji Kawano and Ko Shimamoto
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
  • Year and Date: 2013-03-22
• [Presentation] 細胞膜上でのNLRファミリー免疫受容体PitによるRhoファミリーGタンパク質OsRac1の活性化がイネ免疫に重要である (2012)
  • Author(s): 河野 洋治、林 敬子、赤松 明、宝泉雄介、奥田 淳、中島 綾子、高橋 弘喜、吉田 均, Hann Ling WONG, 川崎 努、島本 功
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] Defensome network in rice immunity Defensome network in rice immunity (2012)
  • Author(s): Yoji Kawano, Akira Akamatsu, Satoshi Hamada and Ko Shimamoto
  • Organizer: ICBP2011年
  • Place of Presentation: 中国 昆明
  • Year and Date: 2012-11-18
• [Presentation] 2012) Palmitoylation plays an important role in the localization and the immune responses of NB-LRR-type R protein Pit (2012)
  • Author(s): Yoji Kawano, Ai Yao, Yusuke Housen, and Ko Shimamoto
  • Organizer: IPMB2012
  • Place of Presentation: 済州島
  • Year and Date: 2012-10-21
• [Presentation] Palmitoylation plays an important role in the localization and the immune responses of NB-LRR-type R protein Pit (2012)
  • Author(s): Yoji Kawano, Ai Yao, Yusuke Housen, and Ko Shimamoto
  • Organizer: IS-MPMI2012
  • Place of Presentation: 京都
  • Year and Date: 2012-07-29
• [Presentation] NB-LRR型Rタンパク質Pitのパルミトイル化が細胞膜局在と免疫誘導に重要である (2012)
  • Author(s): 河野 洋治、八尾 藍、宝泉 雄介、島本 功
  • Organizer: 平成24年度 日本植物病理学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-03-28
• [Presentation] Palmitoylation plays an important role in the localization and the immune responses of NB-LRR-type R protein Pit (2012)
  • Author(s): Yoji Kawano, Ai Yao, Yusuke Housen, and Ko Shimamoto
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-16
• [Presentation] 細胞膜上での免疫受容体 Pit による G タンパク質 OsRac1 の活性化がイネの免疫に重要である (2011)
  • Author(s): 河野 洋治、島本功
  • Organizer: 第75回日本植物学会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-20
• [Presentation] 抵抗性タンパク質PitによるOsSPIKE1を介した低分子量Gタンパク質OsRac1活性化機構の解明
  • Author(s): 河野 洋治
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山大学
• [Presentation] 細胞膜上でのNLRファミリー免疫受容体PitによるRhoファミリーGタンパク質OsRac1の活性化がイネ免疫に重要である
  • Author(s): 河野 洋治
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 福岡県 福岡市
• [Remarks] 島本研
  • URL: http://bsw3.naist.jp/simamoto/simamoto.html
• [Remarks]
  • URL: http://bsw3.aist-nara.ac.jp/simamoto/simamoto.html