Molecular analyses of an alternative C4 abundant transporter, BASS4

• Project/Area Number: 23770047
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Ryukoku University (2012-2013); Hiroshima University (2011)
• Principal Investigator: TSUYOSHI Furumoto 龍谷大学, 文学部, 教授 (30313208)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: C4光合成 / 葉緑体 / ピルビン酸 / 物質輸送 / BASS4 / Ｃ４光合成 / ピルビン酸輸送

Research Abstract

In the CO2-concentration mechanism, performed at several C4 plants, there are a little information on the metabolic transporters located at chlloroplasts. During the identification of a pyruvate transporter, BASS2, located at mesophyl cell plastids, we also found an alternative transporter gene, BASS4, which was highly expressed at C4 Flaveria species in comparison with at C3 Flaveria. To identify the physiological importance, we tried to make transgenic Flaveria, in which Bass4 gene is knocked down. These Bass4-KD plants showed growth defect under the ambient CO2 condition and this difection was complemented under the high CO2 condition, indicates its significant function in the CO2-concentration mechanism.

Research Products

• [Journal Article] A plastidial sodium-dependent pyruvate transporter (2011)
  • Author(s): Furumoto T, Yamaguchi T, Ohshima Y, Nakamura-M, Tsuchida Y, Shimamura M, Ohnishi J, Hata S, Gowik U, Westhoff P, Brautigam A, Weber APM, Izui K
  • Journal Title: Nature Volume: Vol. 476, No. 7361 Issue: 7361 Pages: 472-475
  • DOI: 10.1038/nature10250
  • Peer Reviewed
• [Journal Article] Identification of a plastidial sodium-dependent pyruvate transporter (2011)
  • Author(s): Furumoto T., Yamaguchi T., Ohshima-Ichie Y., Nakamura M., Iwata Y., Shimamura M., Ohnishi J., Hata S., Gowik U., Westhoff P., Bräutigam A., Weber A., Izui K.
  • Journal Title: Nature Volume: 476 Issue: 3 Pages: 472-475
  • DOI: 10.3390/ijms14035312
  • Peer Reviewed
• [Presentation] Identification of plastidial pyruvate transporter and hypothesis of its transporting mechanism (2013)
  • Author(s): Furumoto T.
  • Organizer: International Workshop of Plant Membrane Biology
  • Place of Presentation: 倉敷 岡山
  • Year and Date: 2013-03-28
• [Presentation] C4植物とC3植物のプラスチドの機能的差から見出された新規因子について (2013)
  • Author(s): 古本強
  • Organizer: 植物生理学会サテライトミィーティング第15回オルガネラワークショップ
  • Place of Presentation: 岡山
  • Year and Date: 2013-03-20
• [Presentation] identification of plastidial pyruvate transporter and hypothesis of its transporting mechanism (2013)
  • Author(s): 古本強
  • Organizer: International Workshop of Plant Menbrane Biology
  • Place of Presentation: 岡山県倉敷市
  • Invited
• [Presentation] 植物に温度センサーはあるか?-シロイヌナズナとトウモロコシの温度応答から学ぶ (2012)
  • Author(s): 古本強
  • Organizer: 新学術領域テクニカルワークショップ公開セミナー
  • Place of Presentation: 基礎生物学研究所
  • Year and Date: 2012-10-18
• [Presentation] C4回路上の最後の未知因子、ピルビン酸輸送の分子実態の同定 (2012)
  • Author(s): 古本強
  • Organizer: トランスポーター研究会九州部会
  • Place of Presentation: 福岡歯科医師会館
  • Year and Date: 2012-09-01
• [Presentation] プラスチド局在ナトリウム依存性ピルビン酸輸送体の同定とその後 (2012)
  • Author(s): 古本強
  • Organizer: 日本農薬学会第37回大会「新農薬創生への展望」シンポジウム
  • Place of Presentation: 岡山大学
  • Year and Date: 2012-03-15
• [Presentation] プラスチドで機能するナトリウム依存性ピルビン酸輸送体の分子機構 (2012)
  • Author(s): 古本強
  • Organizer: 酵母細胞シンポジウム
  • Place of Presentation: 広島大学
  • Year and Date: 2012-03-09
• [Presentation] 色素体包膜でのピルビン酸の輸送分子機構 (2012)
  • Author(s): 古本強
  • Organizer: ストレス科学研究シンポジウム
  • Place of Presentation: 岡山大学資源植物科学研究所
  • Year and Date: 2012-03-08
• [Presentation] 冬季栽培性植物に認められる温度感受性についてシロイヌナズナから迫る (2011)
  • Author(s): 古本強
  • Organizer: 第6回ムギ類研究会
  • Place of Presentation: 木原生物学研究所
  • Year and Date: 2011-11-26
• [Presentation] Possible Light Activation Mechanism of the Pyruvate Transporter in C4 Plants (2011)
  • Author(s): Tsuyoshi Furumoto
  • Organizer: Gordon Research Conference
  • Place of Presentation: Les Diablerets, Switzerland
• [Presentation] A novel C4-metabolism-regulator, CP12-3, functioning for the adapting to light fluctuation
  • Author(s): 古本 強
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山大学
  • Invited
• [Presentation] 葉緑体に局在するSLC10ファミリープロテインBASS2はナトリウム依存性ピルビン酸輸送体である
  • Author(s): 古本強
  • Organizer: トランスポーター研究会
  • Place of Presentation: 京都市
• [Book] Identication of the sodium-dependent pyruvate transporter located in plastid envelops (Vision for next-generation pesticides)Journal of Pesticide Science 37 (2012)
  • Author(s): Furumoto T.
  • Publisher: (査読付)