Biochemical analysis of the lightsignaling complex that contains the N-terminal domain of phytochrome B

• Project/Area Number: 23770048
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyushu University
• Principal Investigator: MATSUSHITA Tomonao 九州大学, 大学院・農学研究院, 准教授 (20464399)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: フィトクロム / 植物 / 光受容体 / シグナル伝達 / 環境応答 / 光形態形成 / シロイヌナズナ

Research Abstract

Phytochrome B (phyB) is a plants’ major photoreceptor that regulates various light responses in plants. In order to understand the molecular mechanism of phyB signal transduction, we performed a biochemical analysis to isolate proteins that interact with phyB in vivo. As a result, we identified several splicing-related factors as novel components of phyB signaling complex.

Research Products

• [Journal Article] Deletion of the RS domain of RRC1 impairs phytochrome B signaling in Arabidopsis (2012)
  • Author(s): Shikata H, Nakashima M, Matsuoka K, and Matsushita T
  • Journal Title: Plant Signaling & Behavior Volume: 7 Pages: 933-936
  • Peer Reviewed
• [Journal Article] The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction (2012)
  • Author(s): Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, and Matsushita T
  • Journal Title: The Plant Journal Volume: 70 Pages: 727-738
  • Peer Reviewed
• [Journal Article] Functional analyses of the activation loop of phototropin2 in Arabidopsis (2011)
  • Author(s): Inoue S, Matsushita T, Tomokiyo Y, Matsumoto M, Nakayama KI, Kinoshita T, Shimazaki K
  • Journal Title: Plant Physiology Volume: 56 Pages: 117-128
  • Peer Reviewed
• [Journal Article] A non-covalently attached chromophore can mediate phytochrome B signaling in Arabidopsis (2011)
  • Author(s): Oka Y, Kong SG, Matsushita T
  • Journal Title: Plant & Cell Physiology Volume: 52 Pages: 2088-2102
  • NAID: 10030306272
  • Peer Reviewed
• [Journal Article] A versatile method to prevent transcriptional gene silencing in Arabidopsis thaliana (2011)
  • Author(s): Matsushita T
  • Journal Title: Plant Biotechnology Volume: 28 Pages: 515-519
  • NAID: 10030304205
  • Peer Reviewed
• [Journal Article] A Non- Covalently Attached Chromophore can Mediate Phytochrome B Signaling in Arabidopsis (2011)
  • Author(s): Oka, et al
  • Journal Title: Plant & Cell Physiology Volume: 52 Issue: 12 Pages: 2088-2102
  • DOI: 10.1093/pcp/pcr139
  • Peer Reviewed
• [Journal Article] Functional analyses of the activation loop of phototropin2 in Arabidopsis (2011)
  • Author(s): Inoue S, Matsushita T, Tomokiyo Y, Matsumoto M, Nakayama KI, Kinoshita T, Shimazaki K.
  • Journal Title: Plant Physiology Volume: 156 Issue: 1 Pages: 117-128
  • DOI: 10.1104/pp.111.175943
  • Peer Reviewed
• [Presentation] フィトクロムは選択的スプライシングを調節する (2013)
  • Author(s): 四方明格・松下 智直
  • Organizer: 第54回 日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] フィトクロムは選択的スプライシングを制御する (2013)
  • Author(s): 四方明格
  • Organizer: 第54回 日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] フィトクロムによる選択的スプライシング制御の解析 (2012)
  • Author(s): 牛島智一・柴田磨己・四方明格・大川恭行・松岡健・久原哲・松下智直
  • Organizer: 日本育種学会第121回講演会
  • Place of Presentation: 宇都宮
  • Year and Date: 2012-03-30
• [Presentation] RNA-seq解析によるフィトクロムBの選択的スプライシング制御の標的遺伝子同定とその機能推定 (2012)
  • Author(s): 柴田磨己・四方明格・牛島智一・大川恭行・久原哲・松下智直
  • Organizer: 日本分子生物学会第35回年会
  • Place of Presentation: 福岡
• [Presentation] Phytochrome B controls alternative pre-mRNA splicing through Arabidopsis splicing factor RRC1 (2012)
  • Author(s): 四方明格・牛島智一・柴田磨己・中嶋萌子・孔三根・松岡健・Chentao Lin・松下智直
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: Jeju (Korea)
• [Presentation] 植物の光情報受容体フィトクロムによる細胞内シグナル伝達機構の解析 (2012)
  • Author(s): 松下智直
  • Organizer: 第17回 2012 年日本光生物学協会年会
  • Place of Presentation: 大阪
• [Presentation] Red Light-dependent Alternative Splicing in Phytochrome B Signaling (2012)
  • Author(s): 四方明格・牛島智一・柴田磨己・中嶋萌子・孔三根・Ken Matsuoka・Chentao Lin・松下智直
  • Organizer: The 23rd International Conference on Arabidopsis Research
  • Place of Presentation: Vienna (Austria).
• [Presentation] The SR-like splicing factor RRC1 is required for phytochrome B signal transduction in Arabidopsis (2012)
  • Author(s): 四方明格・柴田磨己・牛島智一・中嶋萌子・孔三根・松岡健・Chentao Lin・松下智直
  • Organizer: The 1st International Symposium on Plant Environmental Sensing
  • Place of Presentation: Nara (Japan)
• [Presentation] Alternative splicing regulation by phytochrome B in Arabidopsis (2012)
  • Author(s): 牛島智一・柴田磨己・四方明格・大川恭行・松岡健・久原哲・松下智直
  • Organizer: The 1st International Symposium on Plant Environmental Sensing
  • Place of Presentation: Nara (Japan).
• [Presentation] シロイヌナズナ RRC1 蛋白質を介した新奇フィトクロム B シグナル伝達機構の解析 (2012)
  • Author(s): 四方明格・柴田磨己・牛島智一・中嶋萌子・孔三根・松岡健・Chentao Lin・松下智直
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
• [Presentation] フィトクロムBによる選択的スプライシング制御の解析 (2012)
  • Author(s): 牛島智一・柴田磨己・四方明格・大川恭行・松岡健・久原哲・松下智直
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
• [Presentation] フィトクロムBは非共有結合により取り込んだ発色団を用いてシグナルを伝達しうる (2012)
  • Author(s): 松下智直・岡義人・孔三根
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
• [Presentation] Red Light-dependent Alternative Splicing in Phytochrome B Signaling (2012)
  • Author(s): Hiromasa Shikata
  • Organizer: The 23rd International Conference on Arabidopsis Research
  • Place of Presentation: Vienna, Austria
• [Presentation] Phytochrome B controls alternative pre-mRNA splicing through Arabidopsis splicing factor RRC1 (2012)
  • Author(s): Hiromasa Shikata
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: Jeju, Korea
• [Presentation] 植物の光情報受容体フィトクロムによる細胞内シグナル伝達機構の解析 (2012)
  • Author(s): 松下智直
  • Organizer: 第17回2012年日本光生物学協会年会
  • Place of Presentation: 大阪
  • Invited
• [Presentation] フィトクロムＢは非共有結合により取り込んだ発色団を用いてシグナルを伝達しうる (2012)
  • Author(s): 松下智直ら
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学（京都市）
• [Presentation] シロイヌナズナRRC1蛋白質を介した新奇フィトクロムBシグナル伝達機構の解析 (2012)
  • Author(s): 四方明格ら
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学（京都市）
• [Presentation] フィトクロムＢによる選択的スプライシング制御の解析 (2012)
  • Author(s): 牛島智一ら
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学（京都市）
• [Presentation] フィトクロムによる選択的スプライシング制御の解析 (2012)
  • Author(s): 牛島智一ら
  • Organizer: 日本育種学会第121回講演会
  • Place of Presentation: 宇都宮大学（宇都宮市）
• [Presentation] Alternative splicing regulation by phytochrome B in Arabidopsis (2012)
  • Author(s): Tomokazu Ushijimaら
  • Organizer: The 1st International Symposium on Plant Environmental Sensing
  • Place of Presentation: 東大寺総合文化センター（奈良市）
• [Presentation] The SR-like splicing factor RRC1 is required for phytochrome B signal transduction in Arabidopsis (2012)
  • Author(s): Hiromasa Shikataら
  • Organizer: The 1st International Symposium on Plant Environmental Sensing
  • Place of Presentation: 東大寺総合文化センター（奈良市）
• [Presentation] Phytochrome B signal transduction in Arabidopsis (2011)
  • Author(s): 松下智直
  • Organizer: International Symposium "Strategies of Plants against Global Environmental Change"
  • Place of Presentation: Kurashiki (Japan)
• [Presentation] 選択的スプライシング制御を介した phytochrome B の光シグナル伝達 (2011)
  • Author(s): 松下智直
  • Organizer: 岡田学術創成研究主催 阿蘇フロンティアサミット
  • Place of Presentation: 阿蘇
• [Presentation] 選択的スプライシング制御を介したphytochrome Bの光シグナル伝達 (2011)
  • Author(s): 松下智直
  • Organizer: 岡田学術創成研究主催 阿蘇フロンティアサミット(招待講演)
  • Place of Presentation: 阿蘇リゾートグランヴィリオホテル（阿蘇市）
• [Presentation] Phytochrome B signal transduction in Arabidopsis (2011)
  • Author(s): Tomonao Matsushita
  • Organizer: International Symposium "Strategies of Plants against Global Environmental Change"(招待講演)
  • Place of Presentation: 芸文館（倉敷市）