Chemical genetics analysis of secondary cell wall formation in protoxylem

• Project/Area Number: 23770054
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: YONEDA Arata 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50553715)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: ケミカルバイオロジー / 道管 / 原生木部道管 / 二次細胞壁 / サルフォンアミド / サルファメチゾール / 表層微小管 / 細胞壁 / 細胞骨格 / 道菅 / パターン形成 / ケミカル・ジェネティクス

Outline of Final Research Achievements

To clarify the mechanisms of secondary cell wall (SCW) formation during xylem vessel differentiation, we have screened the chemicals which affect the SCW formation. As a result, we could identify a compound sulfamethizole (SMZ), which had a novel bioactivity to induce abnormal branching and bending in spiral protoxylem SCWs. SMZ induced aberrant branching and bending cortical microtubules specifically during vessel cell differentiation, meaning that SMZ is a novel inhibitor against SCW formation mechanism in protoxylem and that SMZ could be an novel tool to investigate it further.

Research Products

• [Journal Article] Multiple classes of transcription factors regulate the expression of VASCULAR-RELATED NAC-DOMAIN7, a master switch of xylem vessel differentiation (2015)
  • Author(s): Endo H, Yamaguchi M, Tamura T, Nakano Y, Nishikubo N, Yoneda A, Kato K, Kubo M, Kajita S, Katayama Y, Ohtani M, Demura T
  • Journal Title: Plant and Cell Physiology Volume: 56 Issue: 2 Pages: 242-254
  • DOI: 10.1093/pcp/pcu134
  • Peer Reviewed / Open Access
• [Journal Article] Arabidopsis mitochondrial protein TIM50 affects hypocotyl cell elongation through intracellular ATP level (2012)
  • Author(s): Kumar S, Yoshizumi T, Hongo H, Yoneda A, Hara H, Hamasaki H, Takahashi N, Nagata N, Shimada H, Matsui M
  • Journal Title: Plant Science Volume: 183 Pages: 212-217
  • Peer Reviewed
• [Presentation] Chemical genetic approach for plant cell wall patterning (2015)
  • Author(s): Yoneda A, Kamon E, Demura T
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 木部道管細胞形成の化学遺伝学解析 (2013)
  • Author(s): 家門絵理、米田新、出村拓
  • Organizer: 第７回細胞壁ネットワーク研究会
  • Place of Presentation: つくば市 つくばグランドホテル
• [Presentation] Chemical genetics approach for cell wall biology - characterization of cobtorin - (2012)
  • Author(s): Arata Yoneda
  • Organizer: Workshop on Walls
  • Place of Presentation: University of British Colombia (Vancouver, Canada)
• [Presentation] FESEMを用いたコブトリンの細胞壁構造に対する影響の解析 (2012)
  • Author(s): 米田 新、Tobias Baskin、出村 拓
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都産業大学（京都）
• [Presentation] 新規阻害剤コブトリンにより明らかにされたペクチン側鎖のセルロース微繊維沈着における役割 (2011)
  • Author(s): 米田 新、出村 拓
  • Organizer: 日本生化学会(招待講演)
  • Place of Presentation: 京都国際会館（京都）
• [Presentation] ペクチン側鎖分枝アラビナンのセルロース微繊維沈着における役割 (2011)
  • Author(s): 米田 新、出村 拓
  • Organizer: 日本植物学会
  • Place of Presentation: 東京大学（東京）
• [Presentation] Cobtorin, a novel inhibitor that highlightes a functional interaction between pectin side chains and cellulose microfibrils (2011)
  • Author(s): Arata Yoneda, Taku Demura
  • Organizer: International Conferense on Biosynthesis of Plant Cell Wall
  • Place of Presentation: 淡路島夢舞台（兵庫）
• [Remarks] 奈良先端科学技術大学院大学 バイオサイエンス研究科 植物代謝制御研究室
  • URL: http://bsw3.naist.jp/demura/