Integrated analysis of plant-specific actin-myosin transport by controlling myosin XI velocity artificially
| Project/Area Number |
23770060
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Waseda University (2014)
The Institute of Physical and Chemical Research (2011-2013) |
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Principal Investigator |
TOMINAGA Motoki 早稲田大学, 教育・総合科学学術院, 講師 (50419892)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 原形質流動 / 細胞内輸送 / ミオシン / 植物成長 / 植物ミオシン / 成長制御 / 植物生理学 |
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| Outline of Final Research Achievements |
I have revealed physiological function of cytoplasmic streaming by controlling myosin XI velocity artificially. I generated high- and low-speed chimeric myosin XI by replacing the motor domains of Arabidopsis thaliana myosin XI with those of Chara coralline myosin XI and Homo sapiens myosin Vb, respectively. Surprisingly, the plant sizes of the transgenic Arabidopsis expressing high- and low-speed chimeric myosin XI were larger and smaller, respectively, than that of the wild-type plant. This size change correlated with acceleration and deceleration, respectively, of cytoplasmic streaming. These results strongly suggest that cytoplasmic streaming is a key determinant of plant size.
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Report
(5 results)
Research Products
(43 results)
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[Journal Article] Kinetic mechanism of Nicotiana tabacum myosin-11 defines a new type of a processive motor.2015
Author(s)
Ralph P. Diensthuber, Motoki Tominaga, Matthias Preller,, Falk K. Hartmann, Hidefumi Orii,, Igor Chizhov, Kazuhiro Oiwa,, and Georgios Tsiavaliaris
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Journal Title
The FASEB Journal
Volume: 29
Issue: 1
Pages: 81-94
DOI
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Peer Reviewed
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[Presentation] ミオシンXI の1 分子解析2012
Author(s)
森川高光, 岩城光宏, 伊藤光二, 木村篤, 富永基樹, 池崎圭吾, 小森智貴, 藤田恵介, 中野明彦, 山本啓一, 柳田敏雄
Organizer
日本植物学会第76回大会
Place of Presentation
兵庫県立大学（兵庫）
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