Explore of the regulatory factors involved in the nitrogen metabolic conversion in the ontogeny of amphibians
| Project/Area Number |
23770061
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | University of Toyama |
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Principal Investigator |
KONNO Norifumi 富山大学, 大学院・理工学研究部, 助教 (50507051)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 比較内分泌学 / 尿素合成 / グルココルチコイド / CPS1 / 両生類 / カルバモイルリン酸合成酵素（CPS I） / コルチコステロン / アフリカツメガエル |
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| Research Abstract |
During amphibian metamorphosis, the liver begins to synthesize the urea cycle enzymes necessary to create urea from carbon dioxide and ammonia. We explored the regulatory factor involved in a shift of nitrogen metabolism from ammonotelic to ureotelic. The expression of carbamoyl phosphate synthetase 1 （CPS1) mRNA was first detected at st.47 tadpoles, suggesting that early tadpole produces and excretes urea. We examined the mRNA expression changes in glucocorticoid receptor （GR), CREB, HNF3α, and C/EBPαduring ontogeny. In the results, GR expression was markedly increased in st.44-45 tadpole. We also revealed that corticosterone treatment for Xenopus tadpole （st.44) increased the expression level of CPS I mRNA. Accordingly, CPS I expression （urea synthesis) in early tadpole of Xenopus laevis appears to be mediated by corticosterone and its receptor （GR).
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Report
(3 results)
Research Products
(29 results)
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[Journal Article] The fifth neurohypophysial hormone receptor is structurally related to the V2-type receptor but functionally similar to V1-type receptors2012
Author(s)
Yamaguchi Y, Kaiya H, Konno N, Iwata E, Miyazato M, Uchiyama M, Bell JD, Toop T, Donald JA, Brenner S, Venkatesh B,Hyodo S.
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Journal Title
General and Comparative Endocrinology
Volume: 178
Pages: 519-528
Related Report
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[Journal Article] Uchiyama M, Maejima S, Yoshie S, Kubo Y, Konno N, Joss J2012
Author(s)
Uchiyama M, Maejima S, Yoshie S, Kubo Y, Konno N, Joss J
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Journal Title
Proceedings of the Royal Society B
Volume: 279
Pages: 4795-4802
Related Report
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[Journal Article] The fifth neurohypophysial hormone receptor is structurally related to the V2-type receptor but functionally similar to V1-type receptors.2012
Author(s)
Yamaguchi Y, Kaiya H, Konno N, Iwata E, Miyazato M, Uchiyama M, Bell JD, Toop T, Donald JA, Brenner S, Venkatesh B,Hyodo S.
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Journal Title
General and Comparative Endocrinology
Volume: 178
Pages: 519-528
Related Report
Peer Reviewed
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[Journal Article] Neuropeptide Y in tiger puffer (Takifugu rubripes): distribution, cloning, characterization and mRNA expression responses to prandial condition.2011
Author(s)
Kamijo M, Kojima K, Maruyama K, KonnoN, Motohashi E, Ikegami T, Uchiyama M,Shioda S, Ando H, Matsuda K
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Journal Title
Zoological Science
Volume: 28
Pages: 882-890
NAID
Related Report
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[Journal Article] Ontogeny of ENaC expression in the gills and teh kidneys of the Japanese black salamander (Hynobius nigrescens Stejneger)2011
Author(s)
Uchiyama, M., Kumano, T., Konno, N., Yoshizawa, H., and Matsuda, K
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Journal Title
Journal of Experimental Zoology, Molecular and Developmental Evolution
Volume: 314B
Issue: 2
Pages: 135-145
DOI
Related Report
Peer Reviewed
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[Journal Article] Ghrelin receptor in two species of anuran amphibian, bullfrog (Rana catesbeiana) and Japanese tree frog (Hyla japonica).2011
Author(s)
Kaiya, H., Koizumi, Y., Konno, N., Yamamoto, K., Uchiyama, M., Kangawa, K., Miyazato, M.
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Journal Title
Frontiers in Experimental Endocrinology
Volume: 2
Pages: 1-13
Related Report
Peer Reviewed
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