| Project/Area Number |
23770061
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | University of Toyama |
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Principal Investigator |
KONNO Norifumi 富山大学, 大学院・理工学研究部, 助教 (50507051)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 比較内分泌学 / 尿素合成 / グルココルチコイド / CPS1 / 両生類 / カルバモイルリン酸合成酵素（CPS I） / コルチコステロン / アフリカツメガエル |
|---|
| Research Abstract |
During amphibian metamorphosis, the liver begins to synthesize the urea cycle enzymes necessary to create urea from carbon dioxide and ammonia. We explored the regulatory factor involved in a shift of nitrogen metabolism from ammonotelic to ureotelic. The expression of carbamoyl phosphate synthetase 1 （CPS1) mRNA was first detected at st.47 tadpoles, suggesting that early tadpole produces and excretes urea. We examined the mRNA expression changes in glucocorticoid receptor （GR), CREB, HNF3α, and C/EBPαduring ontogeny. In the results, GR expression was markedly increased in st.44-45 tadpole. We also revealed that corticosterone treatment for Xenopus tadpole （st.44) increased the expression level of CPS I mRNA. Accordingly, CPS I expression （urea synthesis) in early tadpole of Xenopus laevis appears to be mediated by corticosterone and its receptor （GR).
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