| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Research Abstract |
In this project, we aimed to establish an appendicularian chordate Oikoplerua dioica as a simple and useful research model system, as comparable to the nematode C. elegans. We analyzed cellular behaviors during the developmental processes of this organism using real-time imaging techniques. Also we raised an efficient dsRNA-induced RNA interference method in O. dioica. We accumulated morphological data of O. dioica and other species by transmission electron microscopy, uncovering surprising diversity of epidermis among appendicularian species. These methods and data accumulated through this project would strengthen the usefulness of this organism and provide a basis for the plentiful discoveries in future.
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