Ultrastructural investigation of mechanisms of the symbiosis and bleaching of the coral-zooxanthellae symbiotic system

• Project/Area Number: 23770064
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: Kochi University
• Principal Investigator: SEKIDA Satoko 高知大学, 教育研究部総合科学系, 准教授 (70314979)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 微細構造 / サンゴ / 褐虫藻 / 共生 / 白化 / 渦鞭毛藻

Research Abstract

In the present study, the fine structure of the coral-zooxanthellae symbiotic system in the scleractinian Pocillopora damicornis were clarified by freeze-substitution and freeze-fracture electron microscopies. Ultrastructure of healthy corals and corals exposed to higher temperatures were compared. In addition, a cytokinesis process of the symbiotic zooxanthellae was clarified in detail.

Research Products

• [Journal Article] サンゴに共生する褐虫藻の微細構造と生理学的挙動 (2012)
  • Author(s): 関田諭子・奥田一雄
  • Journal Title: 海洋と生物 Volume: 34 (4) Pages: 338-342
• [Journal Article] Molecular anatomy of tunicate senescence: Reversible function of mitochondrial and nuclear genes associated with budding cycles (2012)
  • Author(s): Kawamura K., Kitamura S., Sekida S., Tsuda M., and Sunanaga T
  • Journal Title: Development Volume: 139 Issue: 21 Pages: 4083-4093
  • DOI: 10.1242/dev.083170
  • Peer Reviewed
• [Journal Article] Rhizoid formation in Valonia (Siphonocladales, Chlorophyceae) (2012)
  • Author(s): Elvira P. R., Sekida S. and Okuda K.
  • Journal Title: Phycologia Volume: 51(4) Pages: 391-402
  • Peer Reviewed
• [Journal Article] Inducible growth mode switches influence Valonia rhizoid differentiation (2012)
  • Author(s): Elvira PR, Sekida S, Okuda K
  • Journal Title: Protoplasma Volume: (in press)(掲載決定)(印刷中) Issue: 1 Pages: 407-414
  • DOI: 10.1007/s00709-012-0381-y
  • Peer Reviewed
• [Journal Article] Effects of high pressure in the armored dinoflagellate Scrippsiella hexapraecingula (Peridiniales, Dinophyceae): Changes in thecal plate pattern and microtubule assembly. (2012)
  • Author(s): Sekida S., Takahira M., Horiguchi T. and Okuda K.
  • Journal Title: J. Phycol. Volume: 48 Pages: 163-173
  • Peer Reviewed
• [Journal Article] サンゴに共生する褐虫藻の微細構造と生理学的挙動 (2012)
  • Author(s): 関田諭子
  • Journal Title: 海洋と生物 Volume: 34(4) Pages: 338-342
• [Journal Article] Effects of high pressure in the armored dinoflagellate Scrippsiella hexapraecingula (Peridiniales, Dinophyceae): Changes in thecal plate pattern and microtubule assembly. (2012)
  • Author(s): Sekida S., Takahira M., Horiguchi T. and Okuda K.
  • Journal Title: Journal of Phycology Volume: 48 Pages: 163-173
  • Peer Reviewed
• [Journal Article] Distribution of eelgrass, Zostera japonica Aschers. & Graebn., in a tidal river with reference to some environmental characteristics (2011)
  • Author(s): Himeno S., Yasuoka S., Sekida S., Mine I., Matsui T., Okuda K. and Takahashi M. M.
  • Journal Title: Hikobia Volume: 16 Pages: 85-94
  • NAID: 40019142325
  • Peer Reviewed
• [Journal Article] Band growth and localozation of vesicle exocytosis in the red alga Antithamnion nipponicum (Ceramiales) (2011)
  • Author(s): Mine I, Yuasa K, Uesugi M, Sekida S, Okuda K
  • Journal Title: Eur. J. Phycol. Volume: 46 (3) Pages: 263-270
  • Peer Reviewed
• [Journal Article] Outflow movement during gamete discharge in Bryopsis plumosa (Caulerpales, Chlorophyta) (2011)
  • Author(s): Mine I, Yoshimatsu K, Kobanba A, Sekida S, Okuda K
  • Journal Title: Phycologia Volume: 50 (4) Pages: 363-369
  • Peer Reviewed
• [Journal Article] Microtubule Arrangement during the Formation of a New Growing Tip in the Coenocytic Green Alga Bryopsis plumosa (Caulerpales, Chlorophyta) (2011)
  • Author(s): Mine I, Sekida S and Okuda K
  • Journal Title: Cytologia Volume: 76(2) Pages: 129-135
  • NAID: 10029478355
  • Peer Reviewed
• [Journal Article] Induction of gamete discharge by hypertonic treatment in the green alga Bryopsis plumose (Caulerpales, Chlorophyta) (2011)
  • Author(s): Mine I, Ozaki C, Sekida S, Okuda K
  • Journal Title: Phycological Research Volume: 59 (1) Pages: 70-73
  • Peer Reviewed
• [Journal Article] Outflow movement during gamete discharge in Bryopsis plumosa (Caulerpales, Chlorophyta) (2011)
  • Author(s): Mine I, Yoshimatsu K, Kobanba A, Sekida S, Okuda K
  • Journal Title: Phycologia Volume: 50 Issue: 1 Pages: 363-369
  • DOI: 10.1111/j.1440-1835.2010.00601.x
  • Peer Reviewed
• [Journal Article] Microtubule arrangement during the formation of new growing tip in the coenocytic green alga Bryopsis plumosa (Caulerpales, Chlorophyta). (2011)
  • Author(s): Mine I, Sekida S, Okuda K
  • Journal Title: Cytologia Volume: 76 (2) Pages: 129-135
  • NAID: 10029478355
  • Peer Reviewed
• [Journal Article] Band growth and localization of vesicle exocytosis in the red alga Antithamnion nipponicum (Ceramiales) (2011)
  • Author(s): Mine I, Yuasa K, Uesugi M, Sekida S, Okuda K
  • Journal Title: European Journal of Phycology Volume: 46 Issue: 3 Pages: 263-270
  • DOI: 10.1080/09670262.2011.594524
  • Peer Reviewed
• [Journal Article] Cytoskeletal and ultrastructural studies on regenerating cells from the protoplasts of Valonia (Siphonocladales, Chlorophyceae). (2011)
  • Author(s): Elvira PR, Okuda K
  • Journal Title: Kuroshio Science Volume: 5 Pages: 39-49
  • Peer Reviewed
• [Presentation] ホヤの生殖と加齢 ミトコンドリア遺伝子と核遺伝子の機能相関- (2012)
  • Author(s): 川村 和夫,関田諭子,砂長毅
  • Organizer: 土佐生物学会
  • Place of Presentation: 高知大学(高知県高知市)
  • Year and Date: 2012-12-09
• [Presentation] ミドリゲ目緑藻キッコウグサの分割細胞分裂の光阻害とアクチンフィラメントの動態 (2012)
  • Author(s): 佐藤 友則,湯浅 健, 関田 諭子,奥田 一雄
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学(兵庫県姫路市)
  • Year and Date: 2012-09-17
• [Presentation] 渦鞭毛藻 Spiniferodinium galeiforme の細胞外被構造 (2012)
  • Author(s): 関田諭子,堀口健雄,奥田一雄
  • Organizer: 日本藻類学会第36回大会
  • Place of Presentation: 北海道大学(北海道札幌市)
  • Year and Date: 2012-07-15
• [Presentation] ミドリゲ目海産多核緑藻タンポヤリの分割細胞分裂の過程と特徴 (2012)
  • Author(s): 長谷部有美,関田諭子,奥田一雄
  • Organizer: 生物系三学会大会中国四国支部・島根大会
  • Place of Presentation: 島根大学(島根県島根市)
  • Year and Date: 2012-05-13
• [Presentation] 渦鞭毛藻Spiniferodinium galeiformeの細胞外被構造 (2012)
  • Author(s): 関田諭子
  • Organizer: 日本藻類学会第36回大会
  • Place of Presentation: 北海道大学（札幌市）
• [Presentation] Dictating cell fate by light: Serial growth mode switches in a Valonia rhizoid (2011)
  • Author(s): Paul Rommel Elvira, 関田諭子, 奥田一雄
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2011-09-17
• [Presentation] 黄金色藻 Epipyxis pulchra におけるロリカの微細構造と形成過程 (2011)
  • Author(s): 関田諭子, 島田真知, 奥田一雄
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2011-09-17
• [Presentation] 多核緑藻キッコウグサの細胞同士を連結させる接着細胞の誘導要因 (2011)
  • Author(s): 的野はる菜,関田諭子,奥田一雄
  • Organizer: 生物系三学会大会中国四国支部・香川大会
  • Place of Presentation: 香川大学(香川県高松市)
  • Year and Date: 2011-05-15
• [Presentation] サンゴに共生する渦鞭毛藻の微細構造 (2011)
  • Author(s): 関田諭子,奥田一雄
  • Organizer: 日本藻類学会第35回大会
  • Place of Presentation: 富山大学(富山県富山市)
  • Year and Date: 2011-03-28
• [Presentation] The development and its regulation of cell coverings in armored dinoflagellates (2011)
  • Author(s): Satoko Sekida
  • Organizer: The 6th Asian Pacific Phycological Forum
  • Place of Presentation: 韓国美麗市
• [Presentation] The development and its regulation of cell coverings in armored dinoflagellates (2011)
  • Author(s): Satoko Sekida
  • Organizer: The 6th Asian Pacific Phycological Forum(招待講演)
  • Place of Presentation: 韓国 麗水市
• [Presentation] 黄金色藻Epipyxis pulchraにおけるロリカの微細構造と形態形成 (2011)
  • Author(s): 関田諭子・島田真知・奥田一雄
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京都
• [Remarks] 国立大学法人高知大学 2013南国土佐のうみへんか
  • URL: http://kuroshio.cc.kochi-u.ac.jp/umihenka/index.html