| Project/Area Number |
23770069
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | Kanazawa University (2012)
Suntory Foundation for Life Sciences (2011) |
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Principal Investigator |
SEKIGUCHI Toshio 金沢大学, 環日本海域環境研究センター, 助教 (40378568)
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| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | Calcitonin / Calcitonin receptor / RAMP / ナメクジウオ / Calcitonin 受容体 / 受容体修飾蛋白 / 分子進化 / 比較内分泌学 |
|---|
| Research Abstract |
Calcitonin (CT) family shows high molecular and functional diversification in vertebrates. To elucidate the origin of CT family, CT peptide, CT receptor (CTR), and receptor activity-modifying protein (RAMP) were identified from an amphioxus, Branchiostoma floridae, which is close animal of vertebrates. Furthermore, analysis ofCTR and RAMP expressed in mammalian cell line demonstrated that co-expression of CTR and RAMP is required for ligand activity of CT peptide, and that RAMP is necessary for cell-surface translocation of CTR. These results let us conclusion that CT peptide, CTR, and RAMP have already existed in the common ancestor between vertebrates and amphioxus.
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