Elucidation of the plant sex-determining gene using minute processing of the Y chromosome and expression profiling.
| Project/Area Number |
23770070
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KAZAMA Yusuke 独立行政法人理化学研究所, イオンビーム育種研究チーム, 研究員 (80442945)
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| Research Collaborator |
KAWANO Shigeyuki 東京大学, 大学院新領域創成科学研究科, 教授 (70161338)
ISHII Kotaro 独立行政法人理化学研究所, 生物照射チーム, 特別研究員 (50632965)
CHARLESWORTH Deborah School of Biological Sciences, University of Edinburgh, UK, Institute of Evolutionary Biology
FILATOV Dimitry University of Oxford, UK, Department of Plant Science,
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 重イオンビーム / 雌雄異株植物 / ヒロハノマンテマ / 突然変異 / 植物 / 加速器 / 性染色体 / 性決定遺伝子 |
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| Research Abstract |
Seventeen hermaphrodite mutants and ten asexual mutants that have deletions on the Y chromosome were produced by heavy-ion irradiation in the dioecious plant Silene latifolia. By performing next-generation sequencing, 1741 of Y-linked fragments were identified. The regions for sex determining genes were narrowed down by identifying commonly deleted fragment in hermaphrodite or asexual mutants, respectively. Expression-profiling method using laser microdissection was developed to determine genes expressing in the sex-specific tissues.
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Report
(3 results)
Research Products
(55 results)
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[Journal Article] Narrowing Down the Mapping of Plant Sex-Determination Regions Using New Y Chromosome-Specific Markers and Heavy-Ion-Beam Irradiation-Induced Y Deletion Mutants in Silene latifolia2011
Author(s)
Fujita, N., Torii, C., Ishii, K., Aonuma, W., Shimizu, Y., Kazama, Y., Abe, T., Kawano, S.
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Journal Title
G3
Volume: 2
Issue: 2
Pages: 271-278
DOI
Related Report
Peer Reviewed
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[Journal Article] Production of bisexual flofwer-producing mutants of the dioecious plant, Silene latifolia, by using γ-ray and the heavy-ion.2011
Author(s)
Aonuma W, Ishii K, Torii C, Fujita N, Shimizu Y, Nishihara K, Kazama Y, Abe T, Kawano S
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Journal Title
RIKEN Prog Rep
Volume: 44
Pages: 271-271
Related Report
Peer Reviewed
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