Analysis of the diversity of fungal endophytes with metagenomics
| Project/Area Number |
23770083
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | Kyoto University |
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Principal Investigator |
OSONO Takashi 京都大学, 生態学研究センター, 准教授 (90335307)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | エンドファイト / メタゲノミクス / 多様性 / 林冠生物学 / 次世代シーケンサー / 熱帯林 / 菌類 |
|---|
| Research Abstract |
The alpha diversity and taxonomic composition of endophytic fungi associated with leaves of six tree species were examined in two lowland tropical forests and a subtropical forest in Asia and Australia, using ITS metabarcoding with Roche 454 pyrosequencing. A total of 95,278 fungal reads were clustered into 224 OTUs at 97% similarity threshold. Of the 178 OTUs identified to the phylum, 148 and 30 OTUs belonged to Ascomycota and Basidiomycota, respectively. Major OTUs were common foliar endophytes and pathogens. The low similarities of endophytic fungal assemblages between leaf samples of a single tree species suggested high beta diversity.
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Report
(4 results)
Research Products
(59 results)
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[Presentation] Next-generation sequencing reveals the hyper-diversity of endophytic fungi associated with tree leaves in tropical forests2012
Author(s)
Sakaguchi C, Matsuoka S, Ito K, Hirose D, Yazawa S, Nishimura O, Tanabe A, Toju H, Osono T.
Organizer
The 5th East Asian Federation of Ecological Societies (EAFES) International Congress
Place of Presentation
Ryukoku University, Shiga, Japan (poster session)
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