| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
The alpha diversity and taxonomic composition of endophytic fungi associated with leaves of six tree species were examined in two lowland tropical forests and a subtropical forest in Asia and Australia, using ITS metabarcoding with Roche 454 pyrosequencing. A total of 95,278 fungal reads were clustered into 224 OTUs at 97% similarity threshold. Of the 178 OTUs identified to the phylum, 148 and 30 OTUs belonged to Ascomycota and Basidiomycota, respectively. Major OTUs were common foliar endophytes and pathogens. The low similarities of endophytic fungal assemblages between leaf samples of a single tree species suggested high beta diversity.
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