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Reproductive isolation by sex pheromone

Research Project

Project/Area Number 23770093
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biodiversity/Systematics
Research InstitutionJapan Women's University

Principal Investigator

TSUCHIKANE Yuki  日本女子大学, 理学部, 助教 (20434152)

Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords生殖隔離 / 種分化 / 性フェロモン / ミカヅキモ / 藻類 / ヘテロタリズム / 有性生殖
Outline of Final Research Achievements

reproductive isolation. The Closterium peracerosum-strigosum-littorale complex is a unicellular alga, and it is composed of several reproductively isolated mating groups of heterothallic strains. However, only mating groups IIA, IIB, and IE can be maintained.
I performed field studies to obtained reproductively isolated Closterium peracerosum-strigosum-littorale complexes. Then, I established a new mating group, G. I studied the phylogenetic relationship by using DNA sequences of 1506 group I introns of these mating group. According to the alignment, significant phylogenetic relationships were observed among mating groups IE and G, while mating groups IIA and IIB were distant from groups IE and G. Mating group IE was completely sexually isolated from mating group G. However, partial communication of the sex pheromone existed. Finally, I studied a sex pheromone and sexual isolation by comparing RNA-seq data of mating group G with those of mating group IE

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (47 results)

All 2015 2014 2013 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 2 results) Presentation (33 results) (of which Invited: 6 results) Book (5 results) Remarks (3 results)

  • [Journal Article] A Receptor-like Kinase, Related to Cell Wall Sensor of Higher Plants, is required for Sexual Reproduction in the Unicellular Charophycean Alga, Closterium peracerosum-strigosum-littorale Complex2015

    • Author(s)
      Hirano, Naoko; Marukawa, Yuka; Abe, Jun; Hashiba, Sayuri; Ichikawa, Machiko; Tanabe, Yoichi; Ito, Motomi; Nishii, Ichiro; Tsuchikane, Yuki; Sekimoto, Hiroyuki
    • Journal Title

      Plant and Cell Physiology

      Volume: 印刷中

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Detailed analyses on the parthenospore formation in Closterium moniliferum (Zygnematophyceae, Charophyta).2014

    • Author(s)
      Yuki Tsuchikane, Ayaka Nakai, and Hiroyuki Sekimoto
    • Journal Title

      Phycologia.

      Volume: 53 Issue: 6 Pages: 571-578

    • DOI

      10.2216/14-35r1.1

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 藻類における生殖様式の多様性と進化2013

    • Author(s)
      土金勇樹
    • Journal Title

      分類:BUNRUI

      Volume: 13 Pages: 77-84

    • Related Report
      2013 Research-status Report
  • [Journal Article] New insights into the regulation of sexual reproduction in Closterium2012

    • Author(s)
      Hiroyuki Sekimoto, Jun Abe, and Yuki Tsuchikane
    • Journal Title

      The International Review of Cell and Molecular Biology

      Volume: 297 Pages: 309-338

    • Related Report
      2012 Research-status Report
  • [Journal Article] Zygospore formation between homothallic and heterothallic strains of Closterium.2012

    • Author(s)
      Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H.
    • Journal Title

      Sex Plant Reprod.

      Volume: 25 Issue: 1 Pages: 1-9

    • DOI

      10.1007/s00497-011-0174-z

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] StableNuclear Transformation of Closteriumperacerosum-strigosum-littoralecomplex.2011

    • Author(s)
      Abe, J., Hori, S., Tsuchikane, Y., Kitao, N.,Kato, M. & Sekimoto, H.
    • Journal Title

      Plant Cell Physiol.

      Volume: 52 Issue: 9 Pages: 1676-1685

    • DOI

      10.1093/pcp/pcr103

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] シャジクモ藻類ヒメミカヅキモの有性生殖制御2014

    • Author(s)
      関本 弘之・平野 直子・大槻 涼・ 川井 絢子・土金 勇樹・西山 智明・阿 部 淳
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学(神奈川県川崎市)
    • Year and Date
      2014-09-13 – 2014-09-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒメミカヅキモの-型細胞ゲノム特異的遺伝子CpMinus1の機能解析2014

    • Author(s)
      小宮あゆみ、阿部淳、川井絢子、鈴木穣、豊田敦、藤山秋佐夫、大槻涼、土金勇樹、西山智明、関本弘之
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学(神奈川県川崎市)
    • Year and Date
      2014-09-13 – 2014-09-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒメミカヅキモの性フェロモンと結合するファシクリンIタンパク質の機能解析2014

    • Author(s)
      市原健介、滝口若菜、原真由美、土金勇樹、阿部淳、関本弘之
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学(神奈川県川崎市)
    • Year and Date
      2014-09-13 – 2014-09-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] 接合藻ミカヅキモの種生物学的研究2014

    • Author(s)
      土金勇樹
    • Organizer
      日本藻類学会第38回大会
    • Place of Presentation
      東邦大学(船橋)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 接合藻の生殖様式とその進化2013

    • Author(s)
      土金勇樹
    • Organizer
      第3回微細藻類研究会
    • Place of Presentation
      岡崎コンファレンスセンター
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] A receptor-like kinase responsible for the progress of sexual reproduction of a charophycean alga, Closterium peracerosum-strigosum-littorale complex2013

    • Author(s)
      Sekimoto, H., Hirano, N., Marukawa, Y., Abe, J., Tsuchikane, Y.
    • Organizer
      10th International phycological congress
    • Place of Presentation
      (Florida USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Characterization of a conjugation processes in homothallic Closterium peracerosum-strigosum-littorale complex2013

    • Author(s)
      Tsuchikane, Y., Sekimoto, H.
    • Organizer
      10th International phycological congress
    • Place of Presentation
      (Florida USA
    • Related Report
      2013 Research-status Report
  • [Presentation] 接合藻ジュズミカヅキモ(Closterium moniliferum)における単為胞子形成機構2013

    • Author(s)
      土金勇樹、関本弘之
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2013 Research-status Report
  • [Presentation] 同型配偶子接合を行うヒメミカヅキモの接合過程における性特異的な挙動の探索2013

    • Author(s)
      田中優希、関本弘之、土金勇樹
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2013 Research-status Report
  • [Presentation] 藻類を用いた種分化研究;性フェロモンによる生殖隔離機構2013

    • Author(s)
      土金勇樹
    • Organizer
      第6回日本進化原生生物学研究会
    • Place of Presentation
      大阪
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒメミカヅキモの有性 生殖に関わる受容体型 キナーゼ CpRLK1 の 生理機能2013

    • Author(s)
      平野 直子・土金 勇樹・ 丸川 祐佳・阿部 淳・ 関本 弘之
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2013 Research-status Report
  • [Presentation] シャジクモ藻類ヒメミカヅキモの有性生殖制御2013

    • Author(s)
      関本 弘之・平野 直子・大槻 涼・ 川井 絢子・土金 勇樹・西山 智明・阿 部 淳
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2013 Research-status Report
  • [Presentation] 藻類を用いた種分化研究;性フェロモンによる生殖隔離と生殖様式の進化2013

    • Author(s)
      土金勇樹
    • Organizer
      日本植物分類学会第12回大会、学会奨励賞受賞記念講演
    • Place of Presentation
      千葉
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 生殖隔離に関わる性フェロモンのゲノムレベルでの特徴2013

    • Author(s)
      土金勇樹・西山智明・関本 弘之
    • Organizer
      日本藻類学会第37回大会
    • Place of Presentation
      山梨
    • Related Report
      2012 Research-status Report
  • [Presentation] Sexual recognition between homothallic and heterothallic strains of Closterium2012

    • Author(s)
      Tsuchikane, Y., and Sekimoto, H.
    • Organizer
      International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
    • Place of Presentation
      Nagoya, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] A novel receptor-like protein kinase, CpRLK1, involved in the sexual reproduction of a unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex2012

    • Author(s)
      Hrano N., Marukawa Y., Hashiba S., Tsuchikane Y., Abe J., and Sekimoto H.
    • Organizer
      International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
    • Place of Presentation
      Nagoya, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] フィールドで知るミカヅキモの不思議2012

    • Author(s)
      土金 勇樹
    • Organizer
      平成24年度海外学術調査フォーラム
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Intercellular communication in sexual reproduction of a unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex2012

    • Author(s)
      SekimotoH., Hirano N., Marukawa Y., Tsuchikane Y., Abe J
    • Organizer
      International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
    • Place of Presentation
      Nagoya, Japan
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 藻類を用いた有性生殖の解析 ~ホモタリズムの姉妹接合~2012

    • Author(s)
      土金勇樹
    • Organizer
      第45回日本原生動物学会大会、若手の会ワークショップ
    • Place of Presentation
      姫路
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 接合藻ミカヅキモ (Closterium moniliferum) における単為胞子形成株の生理学的解析2012

    • Author(s)
      土金勇樹・中井彩香・関本弘之
    • Organizer
      日本藻類学会第36回大会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Presentation] 同型配偶子接合を行うヒメミカヅキモの接合過程を追う2012

    • Author(s)
      田中優希・ 土金勇樹・関本弘之
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      姫路
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒメミカヅキモの有性生殖に関わる受容体型タンパク質キナーゼCpRLK1の特性解析2012

    • Author(s)
      平野直子・橋場彩有里・土金勇樹・阿部淳・関本弘之
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      姫路
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒメミカヅキモの性フェロモンPR-IP Inducer結合分子の特性解析2012

    • Author(s)
      関本弘之・滝口若菜・原真由美・高橋紗也香・土金勇樹・阿部淳
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      姫路
    • Related Report
      2012 Research-status Report
  • [Presentation] 接合藻ヒメミカヅキモにおける生殖隔離に関わる性フェロモンの進化2012

    • Author(s)
      土金勇樹・西山智明・関本 弘之
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      姫路
    • Related Report
      2012 Research-status Report
  • [Presentation] 次世代シーケンサーデータのde novoアセンブリー解析2012

    • Author(s)
      西山智明、阿部 淳、土金勇樹、坂山英俊、 関本弘之
    • Organizer
      日本植物生理学会(招待講演)
    • Place of Presentation
      京都産業大学(京都府)
    • Related Report
      2011 Research-status Report
  • [Presentation] 単細胞接合藻類ヒメミカヅキモの性フェロモンPR-IP Inducer結合分子の探索2011

    • Author(s)
      関本弘之、原真由美、高橋紗也香、山田紗也佳、宮城絢乃、土金勇樹、阿部淳
    • Organizer
      日本植物学会
    • Place of Presentation
      東京大学(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 接合藻タテブエ(Penium margaritaceum)のホモタリック株における接合過程の解析2011

    • Author(s)
      土金勇樹、関本弘之
    • Organizer
      日本植物学会
    • Place of Presentation
      東京大学(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 単細胞シャジクモ藻ヒメミカヅキモから見た植物アクアポリンファミリーの進化2011

    • Author(s)
      阿部 淳、瀧 薫、西山 智明、土金 勇樹、関本 弘之
    • Organizer
      日本植物学会
    • Place of Presentation
      東京大学(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] ヒメミカヅキモの有性生殖関連タンパク質CpRLK1の解析2011

    • Author(s)
      平野直子、丸川祐佳、橋場彩有里、土金勇樹、阿部淳、関本弘之
    • Organizer
      日本植物学会
    • Place of Presentation
      東京大学(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] Characterization of a novel receptor-like protein kinase, CpRLK1, involved in the sexual reproduction of a unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex.

    • Author(s)
      Hirano, N., Marukawa, Y., Hashiba, S., Tsuchikane, Y., Abe, J., Sekimoto, H.
    • Organizer
      The 6th Asian Pacific Phycological Forum
    • Place of Presentation
      Yeosu, Korea
    • Related Report
      2011 Research-status Report
  • [Presentation] Hybrid zygospore formation between homothallic and heterothallic strains of Closterium peracerosum-strigosum-littorale complex

    • Author(s)
      Tsuchikane, Y., Tsuchiya, M., Baba, M., Nozaki, H., Sekimoto, H.
    • Organizer
      The 6th Asian Pacific Phycological Forum
    • Place of Presentation
      Yeosu, Korea
    • Related Report
      2011 Research-status Report
  • [Presentation] Identification of aquaporins from the unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex.

    • Author(s)
      Abe, J., Taki, K., Nishiyama, T., Tsuchikane, Y., Sekimoto, H.
    • Organizer
      The 6th Asian Pacific Phycological Forum
    • Place of Presentation
      Yeosu, Korea
    • Related Report
      2011 Research-status Report
  • [Presentation] Closterium and Chara genome and transcriptome analyses.

    • Author(s)
      Abe, J., Sakayama, H., Suzuki, Y., Toyoda, A., Tsuchikane, Y., Sekimoto, H., Nishiyama, T.
    • Organizer
      The International Plant & Animal Genome XX Conference(招待講演)
    • Place of Presentation
      San Diego, CA, USA
    • Related Report
      2011 Research-status Report
  • [Book] 「Mechanism of Sexual Reproduction in Fresh Water Microalgae」 In “Reproductive Biology of Plants”2014

    • Author(s)
      Hiroyuki Sekimoto, Jun Abe, Yuki Tsuchikane
    • Total Pages
      380
    • Publisher
      CRC Press
    • Related Report
      2014 Annual Research Report
  • [Book] 「Sexual reproduction of a unicellular charophycean alga, Closterium peracerosum-strogosum-littorale complex」 In “Sexual Reproduction in Animals and Plants”2014

    • Author(s)
      Hiroyuki Sekimoto, Yuki Tsuchikane, Jun Abe
    • Total Pages
      480
    • Publisher
      Springer.
    • Related Report
      2014 Annual Research Report
  • [Book] Reproductive Biology of Plants “Mechanism of sexual reproduction in fresh water microalgae.“2014

    • Author(s)
      Sekimoto, H., Abe, J., Tsuchikane, Y.
    • Total Pages
      390
    • Publisher
      CRC press
    • Related Report
      2013 Research-status Report
  • [Book] Sexual Reproduction in Animals and Plants “Sexual reproduction of a unicellular charophycean alga, Closterium “peracerosum-strogosum-littorale complex.” " Sexual Reproduction in Animals and Plants2014

    • Author(s)
      Sekimoto, H., Tsuchikane, Y., Abe, J.
    • Total Pages
      480
    • Publisher
      Springer
    • Related Report
      2013 Research-status Report
  • [Book] ミカヅキモの生殖について Sexual reproduction of Closterium2012

    • Author(s)
      土金勇樹・関本弘之
    • Total Pages
      10
    • Publisher
      生物研究社
    • Related Report
      2012 Research-status Report
  • [Remarks] 土金 勇樹  (つちかね ゆうき) のウェブページ

    • URL

      https://sites.google.com/site/tsuchikaneyuki/home

    • Related Report
      2014 Annual Research Report
  • [Remarks] AlgalSex

    • URL

      https://itunes.apple.com/jp/app/algalsex/id339965821?mt=8

    • Related Report
      2013 Research-status Report
  • [Remarks]

    • URL

      https://sites.google.com/site/tsuchikaneyuki/home

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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