| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Prolixibacter denitrificans strain MIC1-1 from a crude-oil sample at an oil well is the first Fe0-corroding nitrate-reducing representative belonging to the order Bacteroidales. Thus, we characterized isolates from oil field and belonging to the phylum Bacteroidetes. Three strains MIC1-1, MIC1-4 and MIC3-8 isolated from crude-oil sample collected at an oil well in Tohoku region were a facultatively aerobic mesophilic chemoheterotrophic non-hydrogenotrophic bacteria. Strains MIC1-1 and MIC1-4 were Fe0-corroding nitrate-reducing bacteria. Strain MIC3-8 was a ferric-reducing bacterium. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MIC1-1 and MIC1-4 were a member of the genus Prolixibacter in the order Bacteroidales, and strain MIC3-8 was a member of the genus Draconibacterium in the order Bacteroidales. On the basis of a polyphasic taxonomic approach, novel species is proposed for strain MIC1-1, to be named Prolixibacter denitrificans.
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