Identifying the unknown novel fungal groups frequently detected in deep-sea and subsurface environments and clarifying their ecological role

• Project/Area Number: 23770098
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: NAGANO Yuriko 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 研究員 (30512917)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 真菌類 / 深海 / メタン冷湧水域 / Cryptomycota / 真菌 / メタン冷湧水 / 化学合成生態系 / 地殻 / 極限環境 / 真核微生物

Research Abstract

Over 300 fungal species were isolated from unique deep-sea environments, including methane cold seeps, 10,000m deep-sea trench, and whale fall sites in this study. Also, many novel unknown fungal groups were revealed in deep-sea environments by analyzing fungal diversity with single cell genomics and next generation sequencer. It was suggested that the group frequently detected from methane cold seep was highly novel and an early diverged group. This study led us to understand fungal diversity and its role in deep-sea environments, through isolating many new and previously unknown species, detecting unknown fungal groups, and using expressed sequence tag (EST) analysis of fungi reported only from deep-sea environments.

Research Products

• [Journal Article] Fungal diversity in deep-sea extreme environments (2012)
  • Author(s): Nagano, Y. & Nagahama, T.
  • Journal Title: Fungal Ecology Volume: 5巻 Issue: 4 Pages: 463-471
  • DOI: 10.1016/j.funeco.2012.01.004
  • Peer Reviewed
• [Journal Article] Screening and Phylogenetic Analysis of Deep-Sea Bacteria Capable of Metabolizing Lignin-Derived Aromatic Compounds (2012)
  • Author(s): Y. Ohta*., S. Nishi, T. Haga, T. Tsubouchi, R. Hasegawa, M. Konishi, Y. Nagano, Y. Tsuruwaka, Y. Shimane, K. Mori, K. Usui, E. Suda, K. Tsutsui, A. Nishimoto, Y. Fujiwara, T. Maruyama, Y. Hatada
  • Journal Title: Open Journal of Marine Science Volume: 2 Issue: 04 Pages: 177-187
  • DOI: 10.4236/ojms.2012.24021
  • Peer Reviewed
• [Journal Article] Unique substrate specificity of a thermostable glycosyl hydrolase from uncultured Anaerolinea, derived from bacterial mat on a subsurface geothermal water stream (2012)
  • Author(s): Masaaki KONISHI, Shinro NISHI, Hideto TAKAMI, Yasuhiro SHIMANE, Yuriko NAGANO, Kozue MORI, Yukari OHTA, Yuji HATADA
  • Journal Title: Biotechnology letters Volume: 34 Issue: 10 Pages: 1887-1893
  • DOI: 10.1007/s10529-012-0983-7
  • Peer Reviewed
• [Journal Article] Abdel‐Wahab, M. A. & Miyazaki, M.Molecular evidence that deep‐branching fungi are major fungal components in deep‐sea methane cold‐seep sediments (2011)
  • Author(s): Nagahama, T., Takahashi, E., Nagano,Y.
  • Journal Title: Environmental Microbiology Volume: 13巻 Issue: 8 Pages: 2359-2370
  • DOI: 10.1111/j.1462-2920.2011.02507.x
  • Peer Reviewed
• [Presentation] Cryptic fungal diversity in deep-sea environments (2013)
  • Author(s): Nagano Y, and Nagahama T
  • Organizer: AMC2013& 13^<th> IMFMS, China National Convention Center (CNCC)
  • Place of Presentation: Beijing, China
  • Year and Date: 2013-08-21
  • Invited
• [Presentation] 深海の未知なる多様性"泳ぐカビ" (2013)
  • Author(s): 長野由梨子、長濱統彦
  • Organizer: 菌学会関東支部年次大会
  • Place of Presentation: 日本大学、千葉県
  • Year and Date: 2013-04-20
  • Invited
• [Presentation] Fungal diversity in deep-sea environments (2012)
  • Author(s): Nagano Y, Nagahama T, Konishi M, Shimane Y, Mori K, Ohta Y, Hatada Y
  • Organizer: 13th International Deep-sea Biology Symposium
  • Place of Presentation: Museum of New Zealand, Wellington, New Zealand(口頭発表)
  • Year and Date: 2012-12-05
• [Presentation] Fungal diversity in deep-sea environments (2012)
  • Author(s): Nagano Y, Nagahama T, Konishi M, Mori K, Shimane Y, Ohta Y, Hatada Y
  • Organizer: BMS 2012 Fungal Interactions
  • Place of Presentation: University of Alicante, Alicante, Spain
  • Year and Date: 2012-09-06
  • Invited
• [Presentation] 深海極限環境中の真菌多様性 (2011)
  • Author(s): 長野由梨子、長濱統彦、小西正朗、森梢、嶋根康広、大田ゆかり、秦田勇二
  • Organizer: 第52回高圧討論会
  • Place of Presentation: 沖縄キリスト教学院、沖縄県(ポスター発表)
  • Year and Date: 2011-11-09
• [Presentation] Fungal diversity in deep-sea environments (2011)
  • Author(s): Nagano Y
  • Organizer: Joint Symposium between MBRC (KIOST) & JAMSTEC
  • Place of Presentation: KIOST, Seoul, Korea
  • Year and Date: 2011-10-17
  • Invited
• [Presentation] Fungal diversity in deep-sea methane cold-seep ecosystems (2011)
  • Author(s): Nagano Y, Takishita K, Nagahama T, Watanabe H, Mori K, Konishi M, Ohta Y and Hatada Y
  • Organizer: The 3rd World Conference on Marine Biodiversity
  • Place of Presentation: Aberdeen Exhibition and Conference Centre, Aberdeen, U.K.(ポスター発表)
  • Year and Date: 2011-09-28
• [Presentation] Fungal diversity in deep-sea methane cold-seep ecosystems (2011)
  • Author(s): Nagano Y, Takishita K, Nagahama T, Watanabe H, Mori K, Tsuruwaka Y, Konishi M, Ohta Y, Hatada Y
  • Organizer: IUMS 2011
  • Place of Presentation: Sapporo Convention Center, Sapporo, Japan
  • Year and Date: 2011-09-09
  • Invited
• [Presentation] 深海・地殻内極限環境における真菌類の多様性 (2011)
  • Author(s): 長野由梨子、長濱統彦、秦田勇二
  • Organizer: 第14回マリンバイオテクノロジー学会
  • Place of Presentation: グランシップ、静岡県
  • Year and Date: 2011-05-28
  • Invited
• [Presentation] Fungal diversity in deep-sea methane cold-seep ecosystems (2011)
  • Author(s): Nagano Y, Takishita K, Nagahama T, Watanabe H, Mori K, Tsuruwaka Y, Konishi M, Ohta Y, Hatada Y
  • Organizer: IUMS 2011(招待講演)
  • Place of Presentation: Sapporo Convention Center, Sapporo, Japan
• [Presentation] 深海・地殻内極限環境における真菌類の多様性 (2011)
  • Author(s): 長野由梨子、長濱統彦、秦田勇二
  • Organizer: 第１４回マリンバイオテクノロジー学会(招待講演)
  • Place of Presentation: グランシップ、静岡県
• [Presentation] 深海極限環境中の真菌多様性 (2011)
  • Author(s): 長野由梨子、長濱統彦、小西正朗、 森梢、 嶋根康広、大田ゆかり、秦田勇二
  • Organizer: 第５２回高圧討論会
  • Place of Presentation: 沖縄キリスト教学院、沖縄県
• [Presentation] Fungal diversity in deep-sea methane cold-seep ecosystems (2011)
  • Author(s): Nagano Y, Takishita K, Nagahama T, Watanabe H, Mori K, Konishi M, Ohta Y and Hatada Y
  • Organizer: The 3rd World Conference on Marine Biodiversity
  • Place of Presentation: Aberdeen Exhibition and Conference Centre, Aberdeen, U.K.
• [Presentation] Cryptic fungal diversity in deep-sea environments.
  • Author(s): Nagano Y, and Nagahama T
  • Organizer: AMC2013& 13th IMFMS
  • Place of Presentation: China National Convention Center (CNCC), Beijing, China
  • Invited
• [Presentation] 深海の未知なる多様性“泳ぐカビ”
  • Author(s): 長野由梨子、長濱統彦
  • Organizer: 菌学会関東支部年次大会
  • Place of Presentation: 日本大学、千葉県
  • Invited
• [Presentation] Fungal diversity in deep-sea environments
  • Author(s): Yuriko Nagano, Takahiko Nagahama, Masaaki Konishi, Kozue Mori, Yasuhiro Shimane, Yukari Ohta, Yuji Hatada
  • Organizer: BMS Annual Scientific Meeting 2012 Fungal Interactions
  • Place of Presentation: Alicante, Spain
  • Invited
• [Presentation] Fungal diversity in deep-sea environments
  • Author(s): Yuriko Nagano
  • Organizer: 2012 Joint Symposium between MBRC(KIOST) & JAMSTEC
  • Place of Presentation: KOIST, Seoul, Korea
  • Invited
• [Presentation] Fungal diversity in deep-sea environments
  • Author(s): Yuriko Nagano, Takahiko Nagahama, Masaaki Konishi, Yasuhiro Shimane, Kozue Mori, Yukari Ohta, Yuji Hatada
  • Organizer: 13th International Deep-sea Biology Symposium
  • Place of Presentation: Wellington, New Zealand
• [Book] Cold-adapted yeasts in deep-sea environments. Cold-adapted Yeasts : Biodiversity, Adaptation Strategies and Biotechnological Significance (2014)
  • Author(s): Nagano Y, Nagahama T and Fumiyoshi Abe
  • Publisher: Springer
• [Book] Cold-adapted yeasts in deep-sea environmentsCold-adapted Yeasts: Biodiversity, Adaptation Strategies and Biotechnological Significance (2014)
  • Author(s): Nagano Y, Nagahama T, Fumiyoshi Abe.
  • Publisher: Springer
• [Book] Cultured and uncultured fungal diversity in deep-sea environments. Biology of marine fungi (2012)
  • Author(s): Nagano Y, and Nagahama T
  • Publisher: Springer
• [Book] Cultured and Uncultured Fungal Diversity in Deep-Sea Environments (Chapter 9 in BIOLOGY OF MARINE FUNGI) (2012)
  • Author(s): Takahiko Nagahama, Yuriko Nagano
  • Total Pages: 15
  • Publisher: Springer