Molecular recognition of HLA-G6 isoform with LILR family

• Project/Area Number: 23770102
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Hokkaido University
• Principal Investigator: KUROKI Kimiko 北海道大学, 大学院・薬学研究院, 助教 (90553313)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: HLA-G6 / 構造解析 / 相互作用解析 / LILR / X線結晶構造解析 / 電子顕微鏡 / NMR / ホモ二量体

Research Abstract

I performed the analysis at the protein level about the interaction with HLA-G6 isoform and the LILR receptor. At first I established the expression system of stable HLA-G6 protein by E.coli. In addition, I performed structural analyses of HLA-G6 and the complex with its receptor, LILRB2, by several techniques (X-ray crystallography, cryo-EM, NMR).

Research Products

• [Journal Article] Structural and functional mosaic nature of MHC class I molecules in their peptide-free form. (2013)
  • Author(s): E.Kurimoto, K.Kuroki, Y.Yamaguchi, M.Yagi-Utsumi, T.Igaki, T.Iguchi, K.Maenaka, and K.Kato
  • Journal Title: Mol. Immunol. Volume: 55 Issue: 3-4 Pages: 393-399
  • DOI: 10.1016/j.molimm.2013.03.014
  • Peer Reviewed
• [Journal Article] The long-term immunosuppressive effects of disulfide-linked HLA-G dimer in mice with collagen-induced arthritis. (2013)
  • Author(s): Kuroki Kら
  • Journal Title: Hum Immunol. Volume: 74 Issue: 4 Pages: 433-438
  • DOI: 10.1016/j.humimm.2012.11.060
  • Peer Reviewed
• [Journal Article] Molecular basis for LLT1 recognition by human CD161 (NKRP1A/KLRB1) (2011)
  • Author(s): Kamishikiryo, ら
  • Journal Title: J.Biol.Chem. Volume: 286 Issue: 27 Pages: 23823-23830
  • DOI: 10.1074/jbc.m110.214254
  • Peer Reviewed
• [Journal Article] Molecular basis for herpesvirus entry mediator recognition by the human immune inhibitory receptor CD160 and its relationship to the cosignaling molecules BTLA and LIGHT (2011)
  • Author(s): Rieko Kojima, et al
  • Journal Title: Journal of Molecular Biology Volume: 413(4) Issue: 4 Pages: 762-772
  • DOI: 10.1016/j.jmb.2011.09.018
  • Peer Reviewed
• [Presentation] ヒト免疫制御受容体LILRB1とリガンドHLA-Gの複合体の結晶構造解析 (2012)
  • Author(s): 黒木喜美子
  • Organizer: 分子生物学会
  • Place of Presentation: 福岡・福岡国際会議場・マリンメッセ福岡
• [Presentation] Structural basis for recognition of nonclassical MHC molecule HLA-G by the LILRB1 (2012)
  • Author(s): Kimiko Kuroki
  • Organizer: The 10th International Symposium for Future Drug Discovery and Medical Care
  • Place of Presentation: The Alumni Hall "Frate", Graduate School of Medicine, Hokkaido University
• [Presentation] Structural basis for recognition of nonclassical MHC molecule HLA-G by the LILRB1 (2012)
  • Author(s): Haruki Matsubara
  • Organizer: 6th International conference on HLA-G
  • Place of Presentation: Paris, Fondation Del Duca
• [Presentation] Immunosuppressive effects of treatment with HLA-G dimer in collagen-induced mouse arthritis (2012)
  • Author(s): Kimiko Kuroki
  • Organizer: 6th International conference on HLA-G
  • Place of Presentation: Paris, Fondation Del Duca
• [Presentation] 免疫抑制分子HLA-Gダイマーの局所投与による関節炎抑制効果の検討 (2011)
  • Author(s): 黒木喜美子
  • Organizer: 分子生物学会
  • Place of Presentation: 横浜・パシフィコ横浜