X-ray crystallography of abscisic acid transporter

Research Project

Project/Area Number	23770104
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Structural biochemistry
Research Institution	The University of Tokyo
Principal Investigator	MIYAKAWA Takuya 東京大学, 大学院・農学生命科学研究科, 助教 (50596559)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000) Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	X線結晶解析 / 生物物理 / 植物 / 蛋白質
Research Abstract	This research has established a method for preparing two types of abscisic acid (ABA) transporters that are key membrane proteins for an intercellular ABA transport. The ABA transporters were overexpressed as membrane fractions by using the yeast expression system of Pichia pastoris. Moreover, detergent screening experiments also showed that ABA transporters were efficiently solubilized with Fos-choline 12 or LDAO among several tested detergents. These results are available with sample preparation for the structural analysis of the ABA transporters, which helps us understand the molecular mechanism of intercellular ABA transport.