Functional analysis of RIG-I and its medical application

• Project/Area Number: 23770113
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Kyoto University
• Principal Investigator: TAKAHASHI Kiyohiro 京都大学, 大学院・薬学研究科, 特定助教 (90399965)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 自然免疫 / RIG-I / インターフェロン / 構造解析 / I型インターフェロン

Research Abstract

RIG-I is a sensor protein that specifically recognize viral RNA and induce early immunity . In the absence of virus RIG-I forms resting form not to induce antiviral activities. Because, those activities are also toxic for our body. I performed functional analysis of sensor protein RIG-I and identified suppression domain of RIG-I that controls RIG-I activation. From this result I suggested the mechanism that how RIG-I is self-controlled its activity .

Research Products

• [Journal Article] 55 Amino acid linker between helicase and carboxyl terminal domains of RIG-I functions as a critical repression domain and determines inter-domain conformation (2011)
  • Author(s): Kageyama, M., Takahasi, K., Narita, R., Hirai, R., Yoneyama, M., Kato, H., Fujita, T.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 415 (1) Pages: 75-81
  • Peer Reviewed
• [Journal Article] 55 amino acid linker between helicase and carboxyl terminal domains of RIG-I functions as a critical repression domain and determines inter-domain conformation (2011)
  • Author(s): Kageyama M, Takahasi K, Narita R, Hirai R, Yoneyama M, Kato H, Fujita T
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 415 Issue: 1 Pages: 75-81
  • DOI: 10.1016/j.bbrc.2011.10.015
  • Peer Reviewed
• [Presentation] ウイルス感染における自然免疫の役割 (2012)
  • Author(s): 高橋清大
  • Organizer: 第三回革新的ナノバイオシンポジウム
  • Place of Presentation: 京都大学薬学部 記念講堂(京都府)
  • Year and Date: 2012-03-04
• [Presentation] ウイルスセンサータンパク質RIG-Iの自己抑制ドメインの同定 (2012)
  • Author(s): 高橋清大
  • Organizer: 第３回 ナノバイオ創薬研究シンポジウム
  • Place of Presentation: 京都大学(京都府)
• [Book] 自然免疫における構造- 機能相関 (2012)
  • Author(s): 高橋清大
  • Total Pages: 4
  • Publisher: メディカルドゥ, トランスレーショナルリサーチを支援するナノバイオ技術と最新創薬応用研究
• [Book] ナノバイオ技術と最新創薬応用研究 (2012)
  • Author(s): 高橋清大
  • Total Pages: 4
  • Publisher: 自然免疫における構造 - 機能相関
• [Remarks]
  • URL: http://www.virus.kyoto-u.ac.jp/Lab/bunshiiden2012/Japanese/99_blank.html