Biological Solid-State NMR for Challenging Samples - Sensitivity Enhancement using Visible Light
| Project/Area Number |
23770114
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
MATSUKI Yoh 大阪大学, たんぱく質研究所, 助教 (70551498)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 固体NMR / 感度向上 / 光化学誘起動的核分極 / 固体核磁気共鳴法 / 光励起核偏極 / 高感度化 / 可視光レーザー / 動的核分極 / 高感度固体NMR |
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| Research Abstract |
Instruments and fundamental techniques have been developed toward establishing a general way for enhancing sensitivity of solid-state NMR via nuclear hyperpolarization based on photo excited dye molecules: photo-chemically induced dynamic nuclear polarization (CIDNP). Using newly developed photo-sensitizer system using flavin derivatives and imidazole embedded in a micelle, enhancement of net nuclear polarization via photo-CIDNP has been observed. It was also found that paramagnetic relaxation enhancement (PRE) effect of the photo-excited dye molecules significantly accelerates the longitudinal relaxation rate of bulk nuclear polarization, enabling rapid signal accumulation or unit-time sensitivity enhancement. The above methods based on light-excited dye overcome a major drawbacks of conventional DNP and PRE techniques: paramagnetic signal broadening, because the paramagnetic species can be quenched by turning the light illumination off during signal acquisition.
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Report
(4 results)
Research Products
(4 results)
