Structural study of telomere maintenance mechanism of RecQ helicases
| Project/Area Number |
23770118
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
KITANO Ken 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (40346309)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | タンパク質 / DNA ヘリカーゼ / RecQ ファミリー / Ｘ線結晶解析 / 構造生物学 / 生物物理学 / 三量体Ｇタンパク質 / 阻害剤 / DNA / ヘリカーゼ / RecQファミリー / ウェルナー症候群 / ブルーム症候群 / 生化学 / Ｘ線結晶構造解析 / 三量体Gタンパク質 |
|---|
| Research Abstract |
RecQ family of DNA helicases WRN (Werner syndrome protein) and BLM (Bloom syndrome protein) play a key role in protecting the genome against deleterious changes. In this study, we determined the first three-dimensional structure of a RecQ C-terminal (RQC) domain from human BLM bound to a phosphate ion. Our result gave insights into the unique DNA-unwinding activity of RecQ helicases toward recombination and repair intermediates (Kim, SY., Hakoshima, T., and Kitano, K., 2013, Sci. Rep., 3, 3294.).
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Report
(4 results)
Research Products
(29 results)
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[Presentation] Protein interaction between NADH oxidase and AhpC (Prx) from Amphibacillus xylanus2013
Author(s)
Mochizuki, D., Arai, T., Hara, K., Matsumoto, T., Kitano, K., Zako, T., Okada, M., Yohda, M., Sato, J., Kawasaki, S., Kanamaru, S., Arisaka, F., Niimura, Y.
Organizer
21st International Conference on Analytical Ultracentrifugation, Hydrodynamics, Thermodynamics and Complementary Methods (AUC2013)
Place of Presentation
静岡県熱海市,ホテルニューアカオ
Year and Date
2013-09-26
Related Report
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[Presentation] Protein interaction between NADH oxidase and AhpC (Prx) from Amphibacillus xylanus.2013
Author(s)
Daichi Mochizuki, Toshiaki Arai, Keita Hara, Takashi Matsumoto, Ken Kitano, Tamotsu Zako, Masafumi Odaka, Masafumi Yohda, Junichi Sato, Shinji Kawasaki, Shuji Kanamaru, Fumio Arisaka, Youichi Niimura
Organizer
21st International Conference on Analytical Ultracentrifugation, Hydrodynamics, Thermodynamics and Complementary Methods（AUC2013）
Place of Presentation
ホテルニューアカオ, 静岡県熱海市
Related Report
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