Structural study of telomere maintenance mechanism of RecQ helicases

• Project/Area Number: 23770118
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: KITANO Ken 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (40346309)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: タンパク質 / DNA ヘリカーゼ / RecQ ファミリー / Ｘ線結晶解析 / 構造生物学 / 生物物理学 / 三量体Ｇタンパク質 / 阻害剤 / DNA / ヘリカーゼ / RecQファミリー / ウェルナー症候群 / ブルーム症候群 / 生化学 / Ｘ線結晶構造解析 / 三量体Gタンパク質

Research Abstract

RecQ family of DNA helicases WRN (Werner syndrome protein) and BLM (Bloom syndrome protein) play a key role in protecting the genome against deleterious changes. In this study, we determined the first three-dimensional structure of a RecQ C-terminal (RQC) domain from human BLM bound to a phosphate ion. Our result gave insights into the unique DNA-unwinding activity of RecQ helicases toward recombination and repair intermediates (Kim, SY., Hakoshima, T., and Kitano, K., 2013, Sci. Rep., 3, 3294.).

Research Products

• [Journal Article] Crystallographic Studies of Human RecQ Helicases WRN and BLM (2014)
  • Author(s): 北野健
  • Journal Title: Nihon Kessho Gakkaishi Volume: 56 Issue: 2 Pages: 133-138
  • DOI: 10.5940/jcrsj.56.133
  • NAID: 130004552886
  • ISSN: 0369-4585, 1884-5576
  • Peer Reviewed / Open Access
• [Journal Article] ヒト RecQ ヘリカーゼ WRN と BLM の結晶構造解析 (2014)
  • Author(s): 北野 健
  • Journal Title: 日本結晶学会誌 Volume: 56(2)
  • NAID: 130004552886
  • Peer Reviewed
• [Journal Article] Structure of the RecQ C-terminal domain of human Bloom syndrome protein. (2013)
  • Author(s): Kim SY, Hakoshima T, Kitano K.
  • Journal Title: Sci Rep. Volume: 3 Issue: 1 Pages: 3294-3294
  • DOI: 10.1038/srep03294
  • NAID: 120005841622
  • Peer Reviewed
• [Presentation] Structural study of Bloom syndrome protein (2014)
  • Author(s): Kim, SY., Hakoshima, T., Kitano, K.
  • Organizer: Biophysical Society 58th Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2014-02-18
• [Presentation] Structural study of Bloom syndrome protein. (2014)
  • Author(s): Sun-Yong Kim, Toshio Hakoshima, Ken Kitano
  • Organizer: Biophysical Society 58th Annual Meeting
  • Place of Presentation: San Francisco, CA, USA.
• [Presentation] RadixinとMT1-MMPとの複合体のX線結晶構造解析 (2013)
  • Author(s): 寺脇慎一,北野健,森智行,青山美樹,箱嶋敏雄
  • Organizer: 平成25年度日本結晶学会年会
  • Place of Presentation: 熊本大学,熊本市
  • Year and Date: 2013-10-12
• [Presentation] Protein interaction between NADH oxidase and AhpC (Prx) from Amphibacillus xylanus (2013)
  • Author(s): Mochizuki, D., Arai, T., Hara, K., Matsumoto, T., Kitano, K., Zako, T., Okada, M., Yohda, M., Sato, J., Kawasaki, S., Kanamaru, S., Arisaka, F., Niimura, Y.
  • Organizer: 21st International Conference on Analytical Ultracentrifugation, Hydrodynamics, Thermodynamics and Complementary Methods (AUC2013)
  • Place of Presentation: 静岡県熱海市,ホテルニューアカオ
  • Year and Date: 2013-09-26
• [Presentation] 膜貫通型プロテアーゼMT1-MMPのERM蛋白質による細胞骨格連結の構造的基盤 (2013)
  • Author(s): 寺脇慎一,北野健,青山美樹,箱嶋敏雄
  • Organizer: 第13回日本蛋白質科学会年会
  • Place of Presentation: とりぎん文化会館,鳥取市
  • Year and Date: 2013-06-13
• [Presentation] Allosteric inhibition of heterotrimeric Gq protein by a small molecule (2013)
  • Author(s): Kitano, K., Nishimura, A., Hakoshima, T., Itoh, H.
  • Organizer: EMBO Conference on Allosteric Interactions in Cell Signaling and Regulation
  • Place of Presentation: Paris, France
  • Year and Date: 2013-05-15
• [Presentation] Radixin と MT1-MMP との複合体のＸ線結晶構造解析 (2013)
  • Author(s): 寺脇慎一, 北野 健, 森 智行, 青山美樹, 箱嶋敏雄
  • Organizer: 平成25年度 日本結晶学会年会
  • Place of Presentation: 熊本大学, 熊本市
• [Presentation] Protein interaction between NADH oxidase and AhpC (Prx) from Amphibacillus xylanus. (2013)
  • Author(s): Daichi Mochizuki, Toshiaki Arai, Keita Hara, Takashi Matsumoto, Ken Kitano, Tamotsu Zako, Masafumi Odaka, Masafumi Yohda, Junichi Sato, Shinji Kawasaki, Shuji Kanamaru, Fumio Arisaka, Youichi Niimura
  • Organizer: 21st International Conference on Analytical Ultracentrifugation, Hydrodynamics, Thermodynamics and Complementary Methods（AUC2013）
  • Place of Presentation: ホテルニューアカオ, 静岡県熱海市
• [Presentation] 膜貫通型プロテアーゼ MT1-MMP の ERM 蛋白質による細胞骨格連結の構造的基盤 (2013)
  • Author(s): 寺脇慎一, 北野 健, 青山美樹, 箱嶋敏雄
  • Organizer: 第13回 日本蛋白質科学会年会
  • Place of Presentation: とりぎん文化会館, 鳥取市
• [Presentation] Allosteric inhibition of heterotrimeric Gq protein by a small molecule. (2013)
  • Author(s): Ken Kitano, Akiyuki Nishimura, Toshio Hakoshima, Hiroshi Itoh.
  • Organizer: EMBO Conference on Allosteric Interactions in Cell Signaling and Regulation
  • Place of Presentation: Institut Pasteur, Paris, France
• [Presentation] Crystallographic Studies of Werner Syndrome DNA Helicase (2012)
  • Author(s): Kitano, K., Kim, S.Y., Hakoshima, T.
  • Organizer: Meeting of the American Crystallographic Association (ACA2012)
  • Place of Presentation: Boston, USA
  • Year and Date: 2012-07-29
• [Presentation] ERM蛋白質による膜貫通型プロテアーゼMT1-MMP認識の構造的基盤 (2012)
  • Author(s): 寺脇慎一,北野健,青山美樹,箱嶋敏雄
  • Organizer: 日本生化学会関東支部例会
  • Place of Presentation: 群馬大学,前橋市
  • Year and Date: 2012-06-23
• [Presentation] 環状ペプチドによる三量体Gタンパク質の阻害機構 (2011)
  • Author(s): 北野健
  • Organizer: 日本学術振興会169委員会第37回研究会
  • Place of Presentation: ゆうぽうと,東京
  • Year and Date: 2011-12-13
• [Presentation] 三量体Gタンパク質の構造生物学と環状ペプチドのドラッグデザイン (2011)
  • Author(s): 北野健
  • Organizer: 包括脳ネットワーク研究会・蛋白研セミナー共催セミナー
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2011-11-22
• [Presentation] Structural study of Werner syndrome DNA helicase (2011)
  • Author(s): Kitano, K., Kim, S.Y., Hakoshima, T.
  • Organizer: The EMBO Meeting
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2011-09-12
• [Presentation] Structural biology of human RecQ DNA helicases (2011)
  • Author(s): Kitano, K.
  • Organizer: The 2nd International Conference on Pharmacy and Advanced Pharmaceutical Sciences
  • Place of Presentation: Yogyakarta, Indonesia
  • Year and Date: 2011-07-19
• [Presentation] 微小管+端集積因子,EB1-CLASPの複合体結晶構造解析 (2011)
  • Author(s): 森智行,北野健,箱嶋敏雄
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: ホテル阪急エキスポパーク,大阪
  • Year and Date: 2011-06-07
• [Presentation] 環状ペプチドによる三量体Gタンパク質の阻害機構 (2011)
  • Author(s): 北野 健
  • Organizer: 日本学術振興会169委員会 第37回研究会(招待講演)
  • Place of Presentation: ゆうぽうと, 東京
• [Presentation] 三量体Gタンパク質の構造生物学と環状ペプチドのドラッグデザイン (2011)
  • Author(s): 北野 健
  • Organizer: 包括脳ネットワーク研究会・蛋白研セミナー共催セミナー(招待講演)
  • Place of Presentation: 岡崎コンファレンスセンター
• [Presentation] Structural study of Werner syndrome DNA helicase. (2011)
  • Author(s): Kitano, K., Kim, S.Y., Hakoshima, T.
  • Organizer: The EMBO Meeting 2011
  • Place of Presentation: Vienna, Austria
• [Presentation] Structural biology of human RecQ DNA helicases. (2011)
  • Author(s): Kitano, K.
  • Organizer: 2nd Int'l Conf. on Pharm. & Adv. Pharm. Sci.(招待講演)
  • Place of Presentation: Yogyakarta, Indonesia
• [Presentation] 微小管＋端集積因子、EB1-CLASPの複合体結晶構造解析 (2011)
  • Author(s): 森 智行, 北野 健, 箱嶋敏雄
  • Organizer: 第11回 日本蛋白質科学会年会
  • Place of Presentation: ホテル阪急エキスポパーク, 大阪
• [Presentation] Crystallographic Studies of Werner Syndrome DNA Helicase.
  • Author(s): Ken Kitano, Sun-Yong Kim, Toshio Hakoshima
  • Organizer: Meeting of the American Crystallographic Association (ACA2012)
  • Place of Presentation: Boston, USA.
• [Presentation] ERM 蛋白質による膜貫通型プロテアーゼ MT1-MMP 認識の構造的基盤
  • Author(s): 寺脇慎一，北野 健，青山美樹，箱嶋敏雄
  • Organizer: 平成24年度 日本生化学会 関東支部例会
  • Place of Presentation: 群馬大学
• [Remarks] Protein Data Bank (PDB)への登録 ①Kim, SY., Hakoshima, T., Kitano, K. (2013), "Structure of BLM RQC domain bound to a phosphate ion." 3WE2.
• [Remarks] リン酸結合型 BLM RQC ドメインの結晶構造
  • URL: http://www.rcsb.org/pdb/explore.do?structureId=3WE2