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Structural study of slective cargo transport by unconventional myosins

Research Project

Project/Area Number 23770119
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionNara Institute of Science and Technology

Principal Investigator

HIRANO Yoshinori  奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50452529)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
KeywordsX線結晶構造解析 / 分子認識 / 積荷輸送 / ミオシン / 微小管 / 細胞内輸送 / 積荷認識 / 軸索ガイダンス / モータータンパク質 / 結晶構造解析 / 分子複合体 / 積み荷認識
Research Abstract

The Cargo transportation by myosins is essential for proper cell phenomena. We peformed a series of structural and biochemicalstudies of the myosin-X tail domain , consisting of MyTH4 and FERM domains in complex with its specific cargo, a netrin receptor DCC (deleted in colorectal cancer). As the result, we found an unexpected binding mode of the DCC peptide, which is distinct from previously reported associations found in radixin/adhesion molecule complexes.
Moreover, we revealed direct interactions between the MyTH4-FERM domain and tubulin C-terminal acidic tails, and identified a positively charged patch of the MyTH4 domain as a tubulin binding region. We also demonstrated that both DCC and integrin bindings interfere with microtubule binding and that DCC binding interferes with integrin binding. Our results provide the molecular basis by which myosin-X facilitates alternative dual binding to cargos and microtubules.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (20 results)

All 2014 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (11 results) Remarks (1 results)

  • [Journal Article] Mechanism of high-affinity abscisic acid binding to PYL9/RCAR12014

    • Author(s)
      Nakagawa M, Kagiyama M, Shibata N, Hirano Y, Hakoshima T
    • Journal Title

      Genes to Cells

      Volume: 19 Pages: 386-404

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism2013

    • Author(s)
      Nasuno, R., Hirano, Y., Itoh, T., Hakoshima, T., Hibi, T., Takagi, H
    • Journal Title

      Proceedings of the National Academy of Sciences of the U.S.A.

      Volume: 110 Pages: 11821-11826

    • NAID

      120005716836

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article]2013

    • Author(s)
      Kagiyama, M., Hirano, Y., Mori, T., Kim, S.Y., Kyozuka, J., Seto, Y., Yamaguchi, S., Hakoshima, T
    • Journal Title

      Genes to Cells

      Volume: 18 Pages: 147-160

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism.2013

    • Author(s)
      Nasuno R, Hirano Y, Itoh T, Hakoshima T, Hibi T, Takagi H.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 110 Issue: 29 Pages: 11821-11826

    • DOI

      10.1073/pnas.1300558110

    • NAID

      120005716836

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structures of D14 and D14L in the strigolactone and karrikin signaling pathways2013

    • Author(s)
      Kagiyama M, Hirano Y, Mori T, Kim SY, Kyozuka J, Seto Y, Yamaguchi S, Hakoshima T
    • Journal Title

      Genes Cells.

      Volume: 18 Issue: 2 Pages: 147-160

    • DOI

      10.1111/gtc.12025

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 植物ホルモンによって誘導されるタンパク質分解のメカニズム2011

    • Author(s)
      村瀬浩司,平野良憲,箱嶋敏雄
    • Journal Title

      実験医学

      Volume: 29 Pages: 1987-1994

    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Journal Article] Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain2011

    • Author(s)
      Hirano,Y., Hatano,T., Takahashi,A., Toriyama,M., Inagaki,N. Hakoshima,T
    • Journal Title

      EMBO J

      Volume: 30 Pages: 2734-2747

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis of cargo recognition by the myosin-X MyTH4–FERM domain2011

    • Author(s)
      Yoshinori Hirano
    • Journal Title

      The EMBO Journal

      Volume: 30 Issue: 13 Pages: 2734-2747

    • DOI

      10.1038/emboj.2011.177

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 酵母の酸化ストレス耐性に関与するN-アセチルトランスフェラーゼMpr1の構造機能解析2013

    • Author(s)
      那須野亮,平野良憲,伊藤貴文,箱嶋敏雄,日び隆雄,高木博史
    • Organizer
      酵母遺伝学フォーラム第46回研究報告会
    • Place of Presentation
      宮城県仙台市東北学院大学土樋キャンパス8号館
    • Year and Date
      2013-09-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 酵母の酸化ストレス耐性に関与するN-アセチルトランスフェラーゼMpr1の構造機能解析2013

    • Author(s)
      那須野亮,平野良憲,伊藤貴文,箱嶋敏雄,日び隆雄,高木博史
    • Organizer
      第13回食品酵素科学研究会
    • Place of Presentation
      大阪府大阪市大阪府立大学I-site難波
    • Year and Date
      2013-09-02
    • Related Report
      2013 Final Research Report
  • [Presentation] Structural and functional analyses of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance2013

    • Author(s)
      那須野亮,平野良憲,伊藤貴文,箱嶋敏雄,日び隆雄,高木博史
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      宮城県仙台市
    • Year and Date
      2013-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 酵母の酸化ストレス耐性に関与するN-アセチルトランスフェラーゼMpr1の構造機能解析2013

    • Author(s)
      那須野 亮, 平野良憲, 伊藤貴文, 箱嶋敏雄, 日び隆雄, 高木博史
    • Organizer
      第13回食品酵素化学研究会
    • Place of Presentation
      大阪府大阪市大阪府立大学 I-site難波
    • Related Report
      2013 Annual Research Report
  • [Presentation] 酵母の酸化ストレス耐性に関与するN-アセチルトランスフェラーゼMpr1の構造機能解析2013

    • Author(s)
      那須野 亮, 平野良憲, 伊藤貴文, 箱嶋敏雄, 日び隆雄, 高木博史
    • Organizer
      酵母遺伝学フォーラム第46回研究報告会
    • Place of Presentation
      宮城県仙台市東北学院大学土樋キャンパス 8号館
    • Related Report
      2013 Annual Research Report
  • [Presentation] 酵母の酸化ストレス耐性に関与するN-アセチルトランスフェラーゼMpr1の構造機能解析2012

    • Author(s)
      須野亮,平野良憲,伊藤貴文,箱嶋敏雄,日び隆雄,高木博史
    • Organizer
      第85会日本生化学会大会
    • Place of Presentation
      福岡県福岡市
    • Year and Date
      2012-12-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain2011

    • Author(s)
      Hirano,Y., Hatano,T, Takahashi,A., Toriyama,M., Inagaki,N. Hakoshima,T.
    • Organizer
      XXII Congress and General Assembly of the International Union of Crystallography
    • Place of Presentation
      マドリッド(スペイン)
    • Year and Date
      2011-08-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Myosin-XのMyTH4-FERMドメインによる積み荷認識機構の構造的基盤2011

    • Author(s)
      平野良憲,畑野大樹,高橋彩,箱嶋敏雄
    • Organizer
      第11回日本蛋白質科学会年会
    • Place of Presentation
      大阪府吹田市ホテル阪急エキスポパーク
    • Year and Date
      2011-06-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain.2011

    • Author(s)
      Hirano, Y., Hatano, T, Takahashi, A., Toriyama, M., Inagaki, N. and Hakoshima, T.
    • Organizer
      XXII Congress and General Assembly of the International Union of Crystallography
    • Place of Presentation
      マドリッド(スペイン)
    • Related Report
      2011 Research-status Report
  • [Presentation] 酵母の酸化ストレス耐性に関与するN-アセチルトランスフェラーゼMpr1の構造機能解析

    • Author(s)
      那須野 亮, 平野 良憲, 伊藤 貴文, 箱嶋 敏雄, 日び 隆雄, 高木 博史
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡県福岡市
    • Related Report
      2012 Research-status Report
  • [Presentation] Structural and functional analyses of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance

    • Author(s)
      那須野 亮, 平野 良憲, 伊藤 貴文, 箱嶋 敏雄, 日び 隆雄, 高木 博史
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      宮城県仙台市
    • Related Report
      2012 Research-status Report
  • [Remarks] 酸化のストレスから酵母を守るカギの酵素「Mpr1」の構造と反応機構を解明

    • URL

      http://www.naist.jp/pressrelease/detail_j/topics/1550/

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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