Structural study of slective cargo transport by unconventional myosins
Project/Area Number |
23770119
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
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Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
HIRANO Yoshinori 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50452529)
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | X線結晶構造解析 / 分子認識 / 積荷輸送 / ミオシン / 微小管 / 細胞内輸送 / 積荷認識 / 軸索ガイダンス / モータータンパク質 / 結晶構造解析 / 分子複合体 / 積み荷認識 |
Research Abstract |
The Cargo transportation by myosins is essential for proper cell phenomena. We peformed a series of structural and biochemicalstudies of the myosin-X tail domain , consisting of MyTH4 and FERM domains in complex with its specific cargo, a netrin receptor DCC (deleted in colorectal cancer). As the result, we found an unexpected binding mode of the DCC peptide, which is distinct from previously reported associations found in radixin/adhesion molecule complexes. Moreover, we revealed direct interactions between the MyTH4-FERM domain and tubulin C-terminal acidic tails, and identified a positively charged patch of the MyTH4 domain as a tubulin binding region. We also demonstrated that both DCC and integrin bindings interfere with microtubule binding and that DCC binding interferes with integrin binding. Our results provide the molecular basis by which myosin-X facilitates alternative dual binding to cargos and microtubules.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism2013
Author(s)
Nasuno, R., Hirano, Y., Itoh, T., Hakoshima, T., Hibi, T., Takagi, H
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Journal Title
Proceedings of the National Academy of Sciences of the U.S.A.
Volume: 110
Pages: 11821-11826
NAID
Related Report
Peer Reviewed
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[Journal Article]2013
Author(s)
Kagiyama, M., Hirano, Y., Mori, T., Kim, S.Y., Kyozuka, J., Seto, Y., Yamaguchi, S., Hakoshima, T
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Journal Title
Genes to Cells
Volume: 18
Pages: 147-160
Related Report
Peer Reviewed
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[Journal Article] Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain2011
Author(s)
Hirano,Y., Hatano,T., Takahashi,A., Toriyama,M., Inagaki,N. Hakoshima,T
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Journal Title
EMBO J
Volume: 30
Pages: 2734-2747
Related Report
Peer Reviewed
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[Presentation] Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain2011
Author(s)
Hirano,Y., Hatano,T, Takahashi,A., Toriyama,M., Inagaki,N. Hakoshima,T.
Organizer
XXII Congress and General Assembly of the International Union of Crystallography
Place of Presentation
マドリッド(スペイン)
Year and Date
2011-08-24
Related Report
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[Presentation] Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain.2011
Author(s)
Hirano, Y., Hatano, T, Takahashi, A., Toriyama, M., Inagaki, N. and Hakoshima, T.
Organizer
XXII Congress and General Assembly of the International Union of Crystallography
Place of Presentation
マドリッド(スペイン)
Related Report
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