Reverse Glycomic Approach for Development of Simple Diagnostic Method of Antitumor-Drug Resistance
| Project/Area Number |
23770120
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
NAKANO Miyako 広島大学, 先端物質科学研究科, 准教授 (40397724)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | 糖質 / 糖鎖 / 癌 / 構造解析 / 薬物耐性 / 白血病 / 微小管脱重合阻害薬 |
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| Research Abstract |
To develop simple diagnostic method of antitumor-drug resistance, we determined the changes in glycosylation that occur on membrane proteins of drug-resistant T-cell acute lymphoblastic leukemia cell line. Drug-resistant cells showed a significant decrease in sialylated glycans. To ascertain if identical alterations occurred on leukemia cell in the blood of leukemia patients, the analytical conditions for LC-ESI MS of glycan structures were investigated. We established the conditions that could analyze their glycan structures using 3 mL of blood. Moreover, we showed that the lectin recognized sialic acids was useful for investigating a simple diagnostic method of drug-resistance acquisition.
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Report
(3 results)
Research Products
(8 results)
