Research Project
Grant-in-Aid for Young Scientists (B)
We developed a platform using budding yeast (S. crevisiae) and green fluorescent protein (GFP) for the rapid construction and evaluation of GPCR variants for structural studies. This platform cut the process time in one third. We produced a stable and highly-expressed histamine H1 receptor (H1R) using this platform. We determined the crystal structure of the H1R in complex with doxepin, an inverse agonist antihistamine. The H1R structure provides key information that should aid the development of highly selective antihistamines.
All 2013 2012 2011 Other
All Journal Article (12 results) (of which Peer Reviewed: 4 results) Presentation (17 results) (of which Invited: 2 results) Remarks (1 results)
YAKUGAKU ZASSHI
Volume: 未定
130003361947
Nature
Volume: 482 Pages: 547-551
120003752107
Microb Cell Fact.
Volume: 11 Pages: 78-78
Micorb, Cell Fact.
Volume: 11 Issue: 1 Pages: 78-78
10.1186/1475-2859-11-78
Methods
Volume: 55 Pages: 281-286
J Med Chem.
Volume: 54 Pages: 8195-8206
J Mol Biol.
Volume: 413 Pages: 762-772
120003660598
Volume: 475 Pages: 65-70
120004225543
Protein Expr Purif.
Volume: 79 Pages: 81-87
120003255719
Pages: 10-24
J. Med. Chem.
http://meneki.phar.kyushu-u.ac.jp/Protein/TOP.html