| Project/Area Number |
23770124
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
MISHIMA Masaki 首都大学東京, 理工学研究科, 准教授 (70346310)
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| Co-Investigator(Renkei-kenkyūsha) |
HAKOSHIMA Toshio 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (00164773)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | NMR / チューブリンチロシンリガーゼ / 微小管 / チューブリン / TTL / 構造解析 |
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| Research Abstract |
We have established the E. coli expression system for the full-length human tubulin tyrosine ligase 1 (TTL1). By using co-expression technique, molecular chaperone was co-expressed to improve the solubility of TTL1, and we succeeded in improvement of the yield about 5 times. We also found that the optimal buffer condition for NMR measurement. We have also developed the high-throughput monitoring system using GFP fused TTL1. Meanwhile, we have established the real-time monitoring system of TTL1 activity by NMR.
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