| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Human immune deficiency virus protease–I (HIVPR) is one of the important drug target proteins for the acquired immune deficiency syndrome. For the efficient drug discovery, it is of importance to obtain structural information of bound water molecules at the active site of HIVPR for the binding of potent inhibitors. Since HIVPR involves the self-degradation, the single-chained and cross-linked HIVPR were designed. The both HIVPR were prepared, and their tertiary structure was determined by X-ray crystallography. The designed HIV-PR will be useful for evaluate the affinity of newly designed inhibitors from kinetic and thermodynamic point of view and preparation of large crystal for neutron crystallography.
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