Applycation of glycopeptide library to structural analysis

• Project/Area Number: 23770134
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Fukushima Medical University
• Principal Investigator: ITO Hiromi 福島県立医科大学, 医学部, 助教 (00450669)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 質量分析 / 構造解析 / 糖鎖 / 糖ペプチド

Research Abstract

Analytical difficulties of glycan structure are arising from the structural complexity of glycans such as variations in branching, linkage and stereo-chemistry. Using glycan and glycopeptide library, I have generated a reference MSn spectra data of the various isomeric glycans for high-throughput MSn analyses. Additionally, MS2 spectra of the permethylated glycan gave the unequivocal fragment ions specific for each isomer (e.g. fucosylated glycan). Thus it was possible to extract an information of the key fragment ion for distinguishing isomers.

Research Products

• [Presentation] Summary of the first to third pilot study of Human Disease Glycomics/Proteomics Initiative (2013)
  • Author(s): Hirimi ITO
  • Organizer: HUPO
  • Place of Presentation: 横浜
• [Presentation]
  • Author(s): 伊藤浩美
  • Organizer: HUPO2013
  • Place of Presentation: 横浜