Applycation of glycopeptide library to structural analysis

Research Project

Project/Area Number	23770134
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Structural biochemistry
Research Institution	Fukushima Medical University
Principal Investigator	ITO Hiromi 福島県立医科大学, 医学部, 助教 (00450669)
Project Period (FY)	2011 – 2013
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	質量分析 / 構造解析 / 糖鎖 / 糖ペプチド
Research Abstract	Analytical difficulties of glycan structure are arising from the structural complexity of glycans such as variations in branching, linkage and stereo-chemistry. Using glycan and glycopeptide library, I have generated a reference MSn spectra data of the various isomeric glycans for high-throughput MSn analyses. Additionally, MS2 spectra of the permethylated glycan gave the unequivocal fragment ions specific for each isomer (e.g. fucosylated glycan). Thus it was possible to extract an information of the key fragment ion for distinguishing isomers.