Studies on the physiological functions of the extrace I lular phosphory lat ion of atypical cadherins

• Project/Area Number: 23770139
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Chiba University
• Principal Investigator: ISHIKAWA Hiroyuki 千葉大学, 大学院・理学研究科, 特任准教授 (00398819)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 翻訳後修飾 / カドヘリン / ゴルジ体キナーゼ / ショウジョウバエ / リン酸化

Research Abstract

Fat is an atypical cadherin that regulates growth and planer cell polarity in Drosophila development. In this study, we focused on the physiological roles of extracellular phosphorylation of Fat cadherin domains, by using transgenic flies. We found that phosphorylation of the third cadherin domain of Fat is required for normal development in Drosophila.

Research Products

• [Journal Article] Rescue of Notch signaling in cells incapable of GDP-L-fucose synthesis by gap junction transfer of GDP-L-fucose in Drosophila (2012)
  • Author(s): Ayukawa T, Matsumoto K, Ishikawa HO, Ishio A, Yamakawa T, Aoyama N, Suzuki T, Matsuno K.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 109(38) Pages: 15318-23
  • Peer Reviewed
• [Journal Article] The Raine syndrome protein FAM20C is a Golgi kinase that phosphorylates bio-mineralization proteins. (2012)
  • Author(s): Ishikawa HO, Xu A, Ogura E, Manning G, Irvine KD.
  • Journal Title: PLoS One Volume: 7 Issue: 8 Pages: e42988-e42988
  • DOI: 10.1371/journal.pone.0042988
  • Peer Reviewed
• [Journal Article] Rescue of Notch signaling in cells incapable of GDP-L-fucose synthesis by gap junction transfer of GDP-L-fucose in Drosophila. (2012)
  • Author(s): Ayukawa T, Matsumoto K, Ishikawa HO, Ishio A, Yamakawa T, Aoyama N, Suzuki T, Matsuno K.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 109 Pages: 15318-23
  • Peer Reviewed
• [Journal Article] Drosophila carrying Pex3 or Pex16 mutations are models of zellweger syndrome that reflect its symptoms associated with the absence of peroxisomes (2011)
  • Author(s): Minoru Nakayama
  • Journal Title: PLoS ONE Volume: 6 Issue: 8 Pages: e22984-e22984
  • DOI: 10.1371/journal.pone.0022984
  • Peer Reviewed
• [Presentation] The Raine syndrome protein FAM20C is a Golgi kinase that phosphorylates bio-mineralizationproteins (2012)
  • Author(s): Ishikawa HO, Xu A, Ogura E, Manning G, Irvine KD.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] In vivo analysis of cadherin domain phosphorylation in the Drosophila Fat (2012)
  • Author(s): Keira Y, Irvine KD, Ishikawa HO.
  • Organizer: The 10th Japanese Drosophila ResearchConference
  • Place of Presentation: 東京慈恵医科大学
  • Year and Date: 2012-10-13
• [Presentation] Identification of a novel Golgi kinase that phosphorylates biomineralization proteins (2012)
  • Author(s): Ishikawa HO, Xu A, Ogura E, Manning G, Irvine KD.
  • Organizer: Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of theJapanSocietyforCel1Biology
  • Place of Presentation: 京都
  • Year and Date: 2012-05-29
• [Presentation] ショウジョウバエ成虫原基におけるfour-jointedのエンハンサー解析 (2012)
  • Author(s): 計良陽子,荒井星矢,森岡早苗,五十嵐美絵,石川裕之
  • Organizer: 日本動物学会第64回関東支部大会
  • Place of Presentation: 東邦大学
  • Year and Date: 2012-03-17
• [Presentation] In vivo analysis of cadherin domain phosphorylation in the Drosophila Fat. (2012)
  • Author(s): Keira Y, Irvine KD, Ishikawa HO.
  • Organizer: 10th Japanese Drosophila Research Conference
  • Place of Presentation: 東京慈恵会医科大学
• [Presentation] Identification of a novel Golgi kinase that phosphorylates biomineralization proteins. (2012)
  • Author(s): Ishikawa HO, Xu A, Ogura E, Manning G, Irvine KD.
  • Organizer: Joint Meeting of JSDB 45th & JSCB 64th
  • Place of Presentation: 神戸
• [Presentation] The Raine syndrome protein FAM20C is a Golgi kinase that phosphorylates biom ineralization proteins. (2012)
  • Author(s): Ishikawa HO, Xu A, Ogura E, Manning G, Irvine KD.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] ショウジョウバエ成虫原基におけるfour-jointedのエンハンサー解析 (2012)
  • Author(s): 計良陽子
  • Organizer: 日本動物学会第64回関東支部大会
  • Place of Presentation: 東邦大学（千葉）
• [Presentation] Enhanceranalysisofthe Golgi kinase four-jointed intransgenicDrosophila (2011)
  • Author(s): 荒井星矢,森岡早苗,五十嵐美絵,計良陽子,石川裕之
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Invivo function of extracellular phosphorylation in the DrosophilacadherinFat (2011)
  • Author(s): 計良陽子,IrvineKD,石川裕之
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] 骨の細胞外基質をリン酸化する新規ゴルジ体キナーゼの同定 (2011)
  • Author(s): 石川裕之,XuA,小倉絵里,ManningG,IrvineKD
  • Organizer: 第36回日本比較内分泌学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-11-23
• [Presentation] Enhancer analysis of the Golgi kinase four-jointed in transgenic Drosophila (2011)
  • Author(s): 荒井星矢
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] In vivo function of extracellular phosphorylation in the Drosophila cadherin Fat (2011)
  • Author(s): 計良陽子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Remarks]
  • URL: http://1ife.s.chiba-u.jp/ishikawa/
• [Remarks] 千葉大学理学研究科生物学教室細胞生理学研究室ホームページ
  • URL: http://life.s.chiba-u.jp/ishikawa/
• [Remarks]
  • URL: http://life.s.chiba-u.jp/ishikawa/