Analysis of interactions among transmembrane proteins required for activation of the yeast HOG MAPK pathway in response to osmotic stress

Research Project

Project/Area Number	23770140
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Functional biochemistry
Research Institution	The University of Tokyo
Principal Investigator	YAMAMOTO Katsuyoshi 東京大学, 医科学研究所, 特任助教 (70508366)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000) Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	細胞情報伝達機構 / シグナル伝達 / 酵母
Research Abstract	Transmembrane (TM) interactions between the tetra-spanning membrane protein Sho1 and the single-path membrane protein Opy2, and the cytoplasmic domain of the osmosensor Hkr1, are required for activation of the Hog1 MAPK via the Hkr1 dependent SHO1 branch pathway in response to osmotic stress. The extracellular cysteine-rich (CR) region of Opy2 is required for interaction with Hkr1/Msb2.