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Mechanisms and roles of spatio-temporal dynamics of SAPKs signaling in determining cell fates.

Research Project

Project/Area Number 23770141
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional biochemistry
Research InstitutionThe University of Tokyo

Principal Investigator

TOMIDA Taichiro  東京大学, 医科学研究所, 助教 (70396886)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsストレス応答 / キナーゼ / イメージング / 細胞死 / ストレス応答シグナル
Research Abstract

(1)Subcellular distribution of stress-activated protein kinase activities were determined by imaging analysis using fluorescent protein-based kinase activity sensors. (2) I found SAPKs are activated either in the cytoplasm or near plasma membrane depending on the type of stresses applied. (3)By manipulating subcellular-localized SAPK activity using inducible protein dimerization system, I found that cell death was induced only when sustained SAPK activity was induced in cytoplasm, while induction of transient SAPK activity resulted in no obvious cell death and showed only slight change in plasma membrane dynamics (membrane brebbing and shape of cell edge). Our data suggest that SAPK mediated signal can determine cell fates depending on the spatial or temporal dynamics of SAPK activity inside cells.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (9 results)

All 2013 2012 2011 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (7 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] The temporal pattern of stimulation determines the extent and duration of MAPK activation in a Caenorhabditis elegans sensory neuron2012

    • Author(s)
      Tomida, T., Oda, S., Takekawa, M., Iino, Y., and Saito, H
    • Journal Title

      Sci Signal

      Volume: 5 Issue: 246 Pages: 76-76

    • DOI

      10.1126/scisignal.2002983

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] 生体内MAPKリン酸化シグナルの動的制御:in vivoイメージングとシステム 工学的アプローチ2013

    • Author(s)
      冨田太一郎、小田茂和、武川陸寛、飯野雄一、斎藤春雄(冨田太一郎)
    • Organizer
      日本細胞生物学会
    • Place of Presentation
      ういんくあいち(愛知県名古屋市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] MAPキナーゼ活性化のinvivoイメージングとシステムエンジニアリング的アプローチによる線虫感覚神経MAPK制御機構の解析2012

    • Author(s)
      冨田太一郎、斎藤春雄
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] キナーゼ活性化のin vivoイメージングとシステムエンジニアリング的アプローチによる線虫感覚神経MAPK制御機構の解析2012

    • Author(s)
      冨田太一郎、斎藤春雄(冨田太一郎)
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 線虫の環境応答行動における情報伝達の可視化2011

    • Author(s)
      冨田太一郎
    • Organizer
      第82回日本動物学会シンポジウム
    • Place of Presentation
      旭川
    • Related Report
      2012 Final Research Report
  • [Presentation] invivoMAPKリン酸化シグナルの可視化による線虫の感覚応答解析2011

    • Author(s)
      冨田太一郎、斎藤春雄
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] 線虫の環境応答行動における情報伝達の可視化~MAPKリン酸化シグナルのライブイメー ジング2011

    • Author(s)
      冨田太一郎、斎藤春雄
    • Organizer
      第82回日本動物学会年会(招待講演)
    • Place of Presentation
      旭川
    • Related Report
      2011 Research-status Report
  • [Presentation] in vivoMAPKリン酸化シグナルの可視化による線虫の感覚応答解析2011

    • Author(s)
      冨田太一郎、斎藤春雄
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Remarks] 東京大学医科学研究所 最新研究成果 生きた動物を用いて細胞内情報伝達を可視化しその動態を解明

    • URL

      http://www.ims.u-tokyo.ac.jp/imsut/jp/research/papers/post_45.php

    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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