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Structural study of maintenance methylation by Dnmt1

Research Project

Project/Area Number 23770145
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional biochemistry
Research InstitutionOsaka University

Principal Investigator

TAKESHITA Kohei  大阪大学, たんぱく質研究所, 招へい研究員 (80346808)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsDNAメチルトランスフェラーゼ1 / X線結晶構造解析 / エピジェネティクス / メチル化模様維持
Research Abstract

The methylated cytosine in genome is epigenetic regulation of gene expression. After the end of DNA replication, newly DNA double strand chain has become hemimethylated state, these cytosine bases in daughter chain is undermodification. Dnmt1 modifies hemimethylated DNA to complete methylated DNA, then Dnmt1 maintain methylated cytosine pattern in genome. In the research project, we aimed to reveal the molecular mechanism of the maintenance methylation by Dnmt1. we succeeded to prepare Dnmt1 and substrate complex protein. Also, we succeeded to determine a full-length Dnmt1 at 3.8 angstrom.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (20 results)

All 2014 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (12 results) Remarks (4 results)

  • [Journal Article] The DNA methyltransferase Dnmt1 directly interacts with the SET and RING finger-associated (SRA) domain of the multifunctional protein Uhrf1 to facilitate accession of the catalytic center to hemi-methylated DNA2014

    • Author(s)
      Berkyurek AC, Suetake I, Arita K, Takeshita K, Nakagawa A, Shirakawa M, Tajima S
    • Journal Title

      J Biol Chem

      Volume: 289(1) Issue: 1 Pages: 379-86

    • DOI

      10.1074/jbc.m113.523209

    • NAID

      120006770872

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The DNA methyltransferase Dnmt1 directly interacts with the SET and RING finger associated (SRA) domain of the multifunctional protein Uhrf1 to facilitate accession of the catalytic center to hemi-methylated DNA.2014

    • Author(s)
      A. C. Berkyurek, I. Suetake, K. Arita, K. Takeshita, A. Nakagawa, M. Shirakawa, and S. Tajima.
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Issue: 7 Pages: 379-386

    • DOI

      10.1074/jbc.m113.528893

    • NAID

      120006770872

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structual insight into maintenance methylation by mouse DNA methyltransferase(Dnmt1)2011

    • Author(s)
      Takeshita K, Suetake I, Yamashita E, Suga M, Narita H, Nakagawa A, and Tajima S
    • Journal Title

      PNAS

      Volume: 108 Issue: 22 Pages: 9055-9059

    • DOI

      10.1073/pnas.1019629108

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural insight into maintenance methylation by mouse DNA methyltransferase 1 (Dnmt1)2011

    • Author(s)
      Takeshita, K., Suetake, I., Yamashita, E., Suga, M., Narita, H., Nakagawa, A., Tajima, S.
    • Journal Title

      Proc.Natl.Acad.Sci.USA

      Volume: 108 Pages: 9055-9059

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Dnmt1(RFTS)とUhrf1(SRA)の相互作用によりDNAは触媒中心に近づけるようになる2014

    • Author(s)
      アーメット・キャン・ベルキュレック、末武 勲、有田 恭平、竹下 浩平、中川 敦史、白川 昌宏、田嶋 正二
    • Organizer
      第8回エピジェネティクス研究会
    • Place of Presentation
      伊藤国際学術研究センター(東京大学構内)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Dnmt1によるDNA維持メチル化活性は領域の大きな再配置を伴う反応である2012

    • Author(s)
      末武勲、中村達郎、ベルキュレクアハメトジャン、竹下浩平、中川敦史、田嶋正三
    • Organizer
      第6回日本エヒジェネティクス研究会年会
    • Place of Presentation
      学術総合センター
    • Related Report
      2013 Final Research Report
  • [Presentation] Dnmt1のX線結晶構造から考察する維持メチル化機構2011

    • Author(s)
      竹下浩平
    • Organizer
      神経科学と構造生物学の融合J(大阪大学蛋白質研究所セミナー・包括脳ネットワーク研究会)
    • Place of Presentation
      (岡崎コンファレンスセンター)
    • Related Report
      2013 Final Research Report
  • [Presentation] X線結晶構造から見るDnmt1のエピジエネティックマーク継承のメカニズム2011

    • Author(s)
      竹下浩平、末武勲、山下栄樹、菅倫寛、成田宏隆、中川敦史、田嶋正三
    • Organizer
      第49回生物物理学会年会
    • Place of Presentation
      兵庫県立大学書写キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] Structural insight into maintenance methylation by mouse DNA methyltransferase 12011

    • Author(s)
      Takeshita K, Suetake, I., Yamashita, E., Suga, Narita, H., Nakagawa, A., Tajima S.
    • Organizer
      International Union of Crystallography (IUCr)
    • Place of Presentation
      Madrid Spain
    • Related Report
      2013 Final Research Report
  • [Presentation] Dnmt1によるメチル化模様維持機構に関する構造生物学的研究2011

    • Author(s)
      竹下浩平、末武勲、山下栄樹、菅倫寛、成田宏隆、中川敦史、田嶋正三
    • Organizer
      第11回日本蛋白質科学会年会
    • Place of Presentation
      ホテル阪急エキスポミーク
    • Related Report
      2013 Final Research Report
  • [Presentation] X線結晶構造から考察するDnmt1による維持メチル化機構2011

    • Author(s)
      竹下浩平、末武勲、山下栄樹、菅倫寛、成田宏隆、中川敦史、田嶋正三
    • Organizer
      第5回日本エヒジェネティクス研究会年会
    • Place of Presentation
      KKR熊本
    • Related Report
      2013 Final Research Report
  • [Presentation] Dnmt1によるメチル化模様維持機構に関する構造生物学的研究2011

    • Author(s)
      竹下浩平、末武勲、山下栄樹、菅倫寛、成田宏隆、中川敦史、田嶋正二
    • Organizer
      第11回日本蛋白質科学会年会
    • Place of Presentation
      ホテル阪急エキスポパーク
    • Related Report
      2011 Research-status Report
  • [Presentation] X線結晶構造から見るDnmt1のエピジェネティックマーク継承のメカニズム2011

    • Author(s)
      竹下浩平、末武勲、山下栄樹、菅倫寛、成田宏隆、中川敦史、田嶋正二
    • Organizer
      第49回生物物理学会年会
    • Place of Presentation
      兵庫県立大学書写キャンパス
    • Related Report
      2011 Research-status Report
  • [Presentation] Dnmt1のX線結晶構造から考察する維持メチル化機構2011

    • Author(s)
      竹下浩平、末武勲、山下栄樹、菅倫寛、成田宏隆、中川敦史、田嶋正二
    • Organizer
      第2回 神経科学と構造生物学の融合(招待講演)
    • Place of Presentation
      岡崎コンファレンスセンター中会議室
    • Related Report
      2011 Research-status Report
  • [Presentation] X線結晶構造から考察するDnmt1による維持メチル化機構

    • Author(s)
      竹下浩平、末武勲、山下栄樹、菅倫寛、成田宏隆、中川敦史、田嶋正二
    • Organizer
      第5回日本エピジェネティクス研究会年会
    • Place of Presentation
      KKRホテル熊本
    • Related Report
      2011 Research-status Report
  • [Presentation] Structural insight into maintenance methylation by mouse DNA methyltransferase 1

    • Author(s)
      Takeshita, K., Suetake, I., Yamashita, E., Suga, M., Narita, H., Nakagawa, A., Tajima, S.
    • Organizer
      International Union of Crystallography (IUCr)
    • Place of Presentation
      Madrid Spain
    • Related Report
      2011 Research-status Report
  • [Remarks] 大阪大学蛋白質研究所・最新研究成果(2011) Structural insight into maintenance methylation by mouse Dnmt1

    • URL

      http://www.protein.osaka-u.ac.jp/jpn/achievement/papers/the-dna-methyltransferase-dnmt.php

    • Related Report
      2013 Final Research Report
  • [Remarks] 大型放射光施設SPring-8プレスリリース・トピックス(2011)ゲノムのメチル化模様維持を触媒する酵素の立体構造を解明-がん細胞を制御する創薬の開発につながる-(プレスリリース)

    • URL

      http://www.spring8.or.jp/ja/news_publications/press_release/2011/110426/

    • Related Report
      2013 Final Research Report
  • [Remarks] 発表論文

    • URL

      http://www.protein.osaka-u.ac.jp/jpn/achievement/papers/structural-insight-into-mainte.php

    • Related Report
      2012 Research-status Report
  • [Remarks] ゲノムのメチル化模様維持を触媒する酵素の立体構造を解明-がん細胞を制御する創薬の開発につながる-

    • URL

      http://www.spring8.or.jp/ja/news_publications/press_release/2011/110426

    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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