| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
The methylated cytosine in genome is epigenetic regulation of gene expression. After the end of DNA replication, newly DNA double strand chain has become hemimethylated state, these cytosine bases in daughter chain is undermodification. Dnmt1 modifies hemimethylated DNA to complete methylated DNA, then Dnmt1 maintain methylated cytosine pattern in genome. In the research project, we aimed to reveal the molecular mechanism of the maintenance methylation by Dnmt1. we succeeded to prepare Dnmt1 and substrate complex protein. Also, we succeeded to determine a full-length Dnmt1 at 3.8 angstrom.
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