Functional analyses of mammalian translocon of the ER
| Project/Area Number |
23770151
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | University of Hyogo |
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Principal Investigator |
KIDA Yuichiro 兵庫県立大学, 大学院・生命理学研究科, 助教 (10423899)
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| Research Collaborator |
SAKAGUCHI Masao 兵庫県立大学, 大学院・生命理学研究科, 教授 (30205736)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | タンパク質膜透過 / シグナル配列 / トランスロコン / 小胞体 / 膜タンパク質 / 細胞小器官 / 膜トポロジー / 生体膜 |
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| Research Abstract |
(1) Marginally hydrophobic sequences, which cannot be integrated into the membrane, can be recognized by the ER translocon. We are now working toward identification of the recognition site of hydrophobic sequences in the Sec61 channel. (2) Novel integration mode of transmembrane sequences of a multi-spanning membrane protein is actually working in the membrane integration via the ER translocon. (3) The details of the translocation-deceleration effect by positively charged residues in the translocating polypeptide chain were determined. (4) The effects of cholesterol and phosphatidylserine for protein translocation and integration via the ER translocon were found.
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Report
(3 results)
Research Products
(58 results)
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[Journal Article] Tail-extension following the termination codon is critical for release of the nascent chain from membrane-bound ribosomes in a reticulocyte lysate cell-free system2013
Author(s)
Takahara, M., Sakaue, H., Onishi, Y., Yamagishi, M., Kida, Y., and Sakaguchi, M
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 430
Issue: 2
Pages: 567-572
DOI
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Peer Reviewed
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[Presentation] ER-targeting Suppression by Short N-terminal Motif of Peroxisomal ABC Transporter, PMP702011
Author(s)
Sakaguchi, M., Iwashita, S., Yamashita, Y., Sakaue, H., and Kida, Y.
Organizer
第63回日本細胞生物学会大会
Place of Presentation
北海道大学（北海道）
Related Report
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[Presentation] Positive charges in translocating polypeptide chain regulate the movement through translocon.2011
Author(s)
Fujita, H., Kida, Y., Yamagishi, M., and Sakaguchi, M.
Organizer
EMBO conference series "Protein transport systems: structures, mechanisms, and medical aspects"
Place of Presentation
Hotel Flamingo (Santa Margherita di Pula, Italy)
Related Report
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