Determination of absorption spectra of reaction intermediates to fully understand the reaction mechanism of cytochrome oxidase

• Project/Area Number: 23770152
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: University of Hyogo
• Principal Investigator: YANAGISAWA SACHIKO 兵庫県立大学, 生命理学研究科, 助教 (60557982)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 金属タンパク質 / 生体エネルギー変換 / 振動分光法 / 酸素活性化 / 振動分光 / 吸収スペクトル

Research Abstract

We isolate electrons from food by digestion and transfer them to dioxygen, generating water molecules. This oxygen reduction reaction is catalyzed by cytochrome oxidase and coupled with proton pumping reaction. The coupling mechanism of these two reactions is still unknown and is investigated by various structural chemical techniques. To understand the mechanisms we need to link all those results in logical manner with a clear benchmark. Absorption spectra of those intermediates can be benchmarks and it is now ready to measure those of intermediates.

Research Products

• [Journal Article] Resonance Raman Study on Indoleamine 2,3-Dioxygenase : Control of Reactivity by Substrate-Binding (2013)
  • Author(s): Sachiko Yanagisawa,Masayuki Hara,Hiroshi Sugimoto,Yoshitsugu Shiro and Takashi Ogura
  • Journal Title: Chem. Phys. Volume: 419 Pages: 178-183
  • Peer Reviewed
• [Journal Article] Interaction of dimeric horse cytochrome c with cyanide ion (2013)
  • Author(s): Ari Dwi Nugraheni,Satoshi Nagao,Sachiko Yanagisawa,Takashi Ogura and Shun Hirota
  • Journal Title: Biol. Inorg. Chem. Volume: 18 Pages: 383-390
  • NAID: 120005716871
  • Peer Reviewed
• [Journal Article] Resonance Raman Study on Indoleamine 2,3-Dioxygenase: Control of Reactivity by Substrate-Binding (2013)
  • Author(s): Sachiko Yanagisawa, Masayuki Hara, Hiroshi Sugimoto, Yoshitsugu Shiro and Takashi Ogura
  • Journal Title: Chem. Phys. Volume: in press Pages: 178-183
  • DOI: 10.1016/j.chemphys.2013.02.032
  • Peer Reviewed
• [Journal Article] Interaction of dimeric horse cytochrome c with cyanide ion (2013)
  • Author(s): Ari Dwi Nugraheni, Satoshi Nagao, Sachiko Yanagisawa, Takashi Ogura and Shun Hirota
  • Journal Title: J Biol Inorg Chem Volume: 18 Issue: 3 Pages: 383-390
  • DOI: 10.1007/s00775-013-0982-8
  • NAID: 120005716871
  • Peer Reviewed
• [Journal Article] Synthesis,Characterization,and Reactivity of Hypochlorito-Iron(III) Porphyrin Complexes (2012)
  • Author(s): Zhiqi Cong,Sachiko Yanagisawa,Takuya Kurahashi,Takashi Ogura,Satoru Nakashima and Hiroshi Fujii
  • Journal Title: Am. Chem. Soc. Volume: 134 Pages: 20617-20620
  • Peer Reviewed
• [Journal Article] Understanding the Mechanism of Short-Range Electron Transfer Using an Immobilized Cupredoxin (2012)
  • Author(s): Stefano Monari,Gianantonio Battistuzzi,Carlo A. Bortolotti,Sachiko Yanagisawa,Katsuko Sato,Chan Li,Isabelle Salard,Dorota Kostrz,Marco Borsari,Antonio Ranieri,Christopher Dennison,and Marco Sola
  • Journal Title: Am. Chem. Soc. Volume: 134 Pages: 11848-11851
  • Peer Reviewed
• [Journal Article] The influence of active site loop mutations on the thermal stability of azurin from Pseudomonas aeruginosa (2012)
  • Author(s): Rita Guzzi,Luigi Sportelli,Sachiko Yanagisawa,Chan Li,Dorota Kostrz,Christopher Dennison
  • Journal Title: Archives of Biochemistry and Biophysics Volume: 521 Pages: 18-23
  • Peer Reviewed
• [Journal Article] Synthesis, Characterization, and Reactivity of Hypochlorito-Iron(III) Porphyrin Complexe (2012)
  • Author(s): Zhiqi Cong, Sachiko Yanagisawa, Takuya Kurahashi, Takashi Ogura, Satoru Nakashima, and Hiroshi Fujii
  • Journal Title: J. Am. Chem. Soc Volume: 134 Issue: 51 Pages: 20617-20620
  • DOI: 10.1021/ja3108774
  • Peer Reviewed
• [Presentation] 基質結合型インドールアミン2,3ジオキシゲナーゼの基質の検出-紫外共鳴ラマン分光法 (2013)
  • Author(s): 柳津幸子、原雅行、杉本宏、城宜嗣、小倉尚志
  • Organizer: 第40回生体分子科学討論会
  • Place of Presentation: 大阪、大阪大学吹田キャンパス
• [Presentation] Detection of tryptophan as bound substrate in indoleamine 2,3-dioxygenase (2013)
  • Author(s): Sachiko Yanagisawa,Masayuki Hara,Hiroshi Sugimoto,Yoshitsugu Shiro and Takashi Ogura
  • Organizer: 4th Georgian Bay International Conference on Bioinorganic Chemistry
  • Place of Presentation: Ontario,Canada
  • Invited
• [Presentation] インドールアミン2,3ジオキシゲナーゼの共鳴ラマンスペクトル : 基質結合による反応性の調節 (2013)
  • Author(s): 柳津幸子、原雅行、杉本宏、城宜嗣、小倉尚志
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 滋賀県、立命館大学くさつ・びわこキャンパス
• [Presentation] Detection of tryptophan as bound substrate in indoleamine 2,3-dioxygenase (2013)
  • Author(s): Sachiko Yanagisawa, Masayuki Hara, Hiroshi Sugimoto, Yoshitsugu Shiro and Takashi Ogura
  • Organizer: 4th Georgian Bay International Conference on Bioinorganic Chemistry
  • Place of Presentation: Parry Sound (CANADA)
  • Invited
• [Presentation] インドールアミン2,3ジオキシゲナーゼの共鳴ラマンスペクトル: 基質結合による反応性の調節 (2013)
  • Author(s): 柳澤幸子・原 雅行・杉本 宏・城 宜嗣・小倉尚志
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学 くさつ・びわこキャンパス（滋賀県）
• [Presentation] へムをへム垂線のまわりに900回転した時の共鳴ラマンスペクトル変化 : へムオキシゲネース (2012)
  • Author(s): Sachiko Yanagisawa,Hiroshi Fujii,Saburo Neya,Takashi Ogura and Teizo Kitagawa,"Effects of Heme Rotation around the Heme Normal by 90 0 on Resonance Raman Spectra of Heme Proteins; Observation for Heme Oxygenase
  • Organizer: 第50回生物物理学会年会
  • Place of Presentation: 名古屋、名古屋大学東山キャンパス
• [Presentation] Effects of Heme Rotation around the Heme Normal by 90 0 on Resonance Raman Spectra of Heme Proteins; Observation for Heme Oxygenase (2012)
  • Author(s): Sachiko Yanagisawa,Hiroshi Fujii,Saburo Neya,Takashi Ogura,and Teizo Kitagawa
  • Organizer: ICORS 2012
  • Place of Presentation: インド・バンガロール
  • Invited
• [Presentation] Dioxygenation reaction of indoleamine 2,3-dioxygenase as studied by resonance Raman spectroscopy (2012)
  • Author(s): Sachiko Yanagisawa,Masayuki Hara,Hiroshi Sugimoto,Yoshitsugu Shiro and Takashi Ogura
  • Organizer: ICPP-7
  • Place of Presentation: 韓国・チェジュ島
  • Invited
• [Presentation] 共鳴ラマン分光法によるインドールアミン2,3ジオキシゲナーゼの反応機構の研究 (2012)
  • Author(s): 柳津幸子、原雅行、杉本宏、城宜嗣、小倉尚志
  • Organizer: 第39回生体分子科学討論会
  • Place of Presentation: 仙台、東北大学
• [Presentation] ヘムをヘム垂線のまわりに90°回転した時の共鳴ラマンスペクトル変化：ヘムオキシゲネース (2012)
  • Author(s): Sachiko Yanagisawa, Hiroshi Fujii, Saburo Neya, Takashi Ogura and Teizo Kitagawa
  • Organizer: 第50回生物物理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス（愛知県）
• [Presentation] Effects of Heme Rotation around the Heme Normal by 90 ° on Resonance Raman Spectra of Heme Proteins; Observation for Heme Oxygenase (2012)
  • Author(s): Sachiko Yanagisawa, Hiroshi Fujii, Saburo Neya, Takashi Ogura, and Teizo Kitagawa
  • Organizer: ICORS 2012
  • Place of Presentation: バンガロール（インド）
  • Invited
• [Presentation] Dioxygenation reaction of indoleamine 2,3-dioxygenase as studied by resonance Raman spectroscopy (2012)
  • Author(s): Sachiko Yanagisawa, Masayuki Hara, Hiroshi Sugimoto, Yoshitsugu Shiro and Takashi Ogura
  • Organizer: ICPP-7
  • Place of Presentation: チェジュ島（韓国）
  • Invited
• [Presentation] 共鳴ラマン分光法によるインドールアミン2,3ジオキシゲナーゼの反応機構の研究 (2012)
  • Author(s): 柳澤 幸子、原 雅行、杉本 宏、城 宜嗣、小倉 尚志
  • Organizer: 第39回生体分子科学討論会
  • Place of Presentation: 東北大学（宮城県）
• [Presentation] Resonance Raman Study on CO-bound Indoleamine 2,3-Dioxygenase with Tryptophan (2011)
  • Author(s): Sachiko Yanagisawa,Hiroshi Sugimoto,Yoshitsugu Shiro,Takashi Ogura
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 姫路
• [Presentation] Resonance Raman Study on Ligand bound Forms of Indoleamine 2,3-Dioxygenase (2011)
  • Author(s): Masayuki Hara,Sachiko Yanagisawa,Hiroshi Sugimoto,Yoshitsugu Shiro,Takashi Ogura
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 姫路
• [Presentation] Resonance Raman Study on Cytochrome c Oxidase Reconstituted in Phospholipid Vesicles (2011)
  • Author(s): Takashi Nomura,Sachiko Yanagisawa,Kyoko Shinzawa-Ito,Shinya Yoshikawa,Takashi Ogura
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 姫路
• [Presentation] 共鳴ラマン分光法によるインドールアミン2、3ジオキシゲナーゼの光学異性依存的基質相互作用の検出 (2011)
  • Author(s): 柳津幸子、杉本宏、城宜嗣、小倉尚志
  • Organizer: 第38回生体分子科学討論会
  • Place of Presentation: 筑波
• [Presentation] Resonance Raman Study on Cytochrome c Oxidase Reconstituted in Phospholipid Vesicles (2011)
  • Author(s): Takashi Nomura, Sachiko Yanagisawa, Kyoko Shinzawa-Ito, Shinya Yoshikawa, Takashi Ogura
  • Organizer: 第49回日本生物物理学会
  • Place of Presentation: 兵庫県立大学姫路書写キャンパス（兵庫県）
• [Presentation] Resonance Raman Study on CO-Bound Indoleamine 2,3-Dioxygenase with Tryptophan (2011)
  • Author(s): Sachiko Yanagisawa, Hiroshi Sugimoto, Yoshitsugu Shiro, Takashi Ogura
  • Organizer: 第49回日本生物物理学会
  • Place of Presentation: 兵庫県立大学姫路書写キャンパス（兵庫県）
• [Presentation] Resonance Raman Study on Ligand-Bound Forms of Indoleamine 2,3-Dioxygenase (2011)
  • Author(s): Masayuki Hara, Sachiko Yanagisawa, Hiroshi Sugimoto, Yoshitsugu Shiro, Takashi Ogura
  • Organizer: 第49回日本生物物理学会
  • Place of Presentation: 兵庫県立大学姫路書写キャンパス（兵庫県）
• [Presentation] 共鳴ラマン分光法によるインドールアミン2,3ジオキシゲナーゼの光学異性依存的基質相互作用の検出 (2011)
  • Author(s): 柳澤幸子、杉本宏、城宜嗣、小倉尚志
  • Organizer: 第38回生体分子科学討論会
  • Place of Presentation: 筑波大学大学会館（茨城県）