Comprehensive analysis of Bacillus subtilis ATP synthase

• Project/Area Number: 23770157
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Rikkyo University
• Principal Investigator: Kato-Yamada Yasuyuki 立教大学, 理学部, 教授 (80386507)
• Project Period (FY): 2011-04-28 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 活性調節 / 阻害 / 生体エネルギー変換 / ヌクレオチド結合 / 脱共役 / 酸化的リン酸化 / 調節 / ATP / 酵素 / 構造機能相関 / アロステリック

Outline of Final Research Achievements

(1) Biochemical analysis of Bacillus subtilis F1-ATPase (BF1) revealed that BF1 is strongly inhibited by MgADP inhibition and the "inhibitory" epsilon subunit acts as an activator in BF1. (2) It was revealed that the strong MgADP inhibition of BF1 is not due to the low ATP binding affinity of noncatalytic sites. (3) Overexpression system of BFoF1 was successfully constructed. (4) Bacillus subtilis strain containing an ATP-sensor protein (ATeam) was constructed. (5) Physiological conditions where the regulation of BFoF1 by the epsilon subunit is prominent were revealed.

Research Products

• [Journal Article] High affinity nucleotide-binding mutant of the ε subunit of thermophilic F1-ATPase (2016)
  • Author(s): Yasuyuki Kato-Yamada
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 469 Issue: 4 Pages: 1129-1132
  • DOI: 10.1016/j.bbrc.2015.12.121
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Severe MgADP Inhibition of Bacillus subtilis F1-ATPase Is Not Due to the Absence of Nucleotide Binding to the Noncatalytic Nucleotide Binding Sites (2014)
  • Author(s): Toru Ishikawa, Yasuyuki Kato-Yamada
  • Journal Title: PLOS ONE Volume: 9 Issue: 9 Pages: e107197-e107197
  • DOI: 10.1371/journal.pone.0107197
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] epsilon Subunit of Bacillus subtilis F1-ATPase relieves MgADP inhibition (2013)
  • Author(s): Junya Mizumoto, Yuka Kikuchi, Yo-hei Nakanishi, Naoto Mouri, Anrong Cai, Tokushiro Ohta, Takamitsu Haruyama, Yasuyuki Kato-Yamada
  • Journal Title: PLoS ONE Volume: 8 Issue: 8 Pages: e73888-e73888
  • DOI: 10.1371/journal.pone.0073888
  • Peer Reviewed
• [Presentation] 枯草菌F1-ATPaseに於けるDELSEED領域の機能解析 (2015)
  • Author(s): 高田浩志、山田康之
  • Organizer: 第53回 日本生物物理学会年会
  • Place of Presentation: 金沢大学角間キャンパス（石川県・金沢市）
  • Year and Date: 2015-09-13
• [Presentation] 枯草菌F1-ATPaseに於けるDELSEED領域の機能解析 (2014)
  • Author(s): 高田浩志、山田康之
  • Organizer: 日本生体エネルギー研究会第40回討論会
  • Place of Presentation: 愛媛大学南加記念ホール（愛媛県・松山市）
  • Year and Date: 2014-12-11 – 2014-12-13
• [Presentation] Role of Noncatalytic Sites of Bacillus subtilis F1-ATPase (2014)
  • Author(s): Toru Ishikawa, Yasuyuki Kato-Yamada
  • Organizer: Tokyo ATPase Workshop
  • Place of Presentation: 東京大学武田先端知ビル（東京都・文京区）
  • Year and Date: 2014-06-02 – 2014-06-03
• [Presentation] 枯草菌F1-ATPaseの非触媒部位とADP阻害の関係性 (2012)
  • Author(s): 石川 透、山田 康之
  • Organizer: 日本生体エネルギー研究会 第38回討論会
  • Place of Presentation: 岡山大学薬学部
• [Presentation] 枯草菌FoF1-ATP合成酵素の機能解析 (2012)
  • Author(s): 多賀名 智昭、鈴木 祥太、河村 富士夫、山田 康之
  • Organizer: 日本生体エネルギー研究会 第38回討論会
  • Place of Presentation: 岡山大学薬学部
• [Presentation] epsilon Subunit suppresses ADP-inhibition of Bacillus subtilis F1-ATPase (2012)
  • Author(s): Junya Mizumoto, Yuka Kikuchi, Yasuyuki Kato-Yamada
  • Organizer: 17th European Bioenergetics Conference
  • Place of Presentation: Albert-Ludwigs-Universitaet in Freiburg im Breisgau, Freiburg im Breisgau, Germany
• [Presentation] ATP合成酵素におけるADP阻害とεサブユニットによる阻害の関係 (2011)
  • Author(s): 水本純弥、菊池有夏、山田康之
  • Organizer: 日本生体エネルギー研究会 第37回討論会
  • Place of Presentation: 京都産業大学神山ホール
• [Remarks] 立教大学理学部生命理学科山田研究室ホームページ
  • URL: http://www2.rikkyo.ac.jp/web/katoyama/
• [Remarks] 立教大学理学部生命理学科山田研究室ホームページ
  • URL: http://www2.rikkyo.ac.jp/web/katoyama/index.html
• [Remarks] 立教大学理学部生命理学科山田研究室ホームページ
  • URL: http://www2.rikkyo.ac.jp/web/katoyama/
• [Remarks] 立教大学理学部生命理学科山田研究室ホームページ
  • URL: http://www2.rikkyo.ac.jp/web/katoyama/
• [Remarks]
  • URL: http://www2.rikkyo.ac.jp/~katoyama/