Comprehensive analysis of Bacillus subtilis ATP synthase
Project/Area Number |
23770157
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Functional biochemistry
|
Research Institution | Rikkyo University |
Principal Investigator |
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Project Period (FY) |
2011-04-28 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | 活性調節 / 阻害 / 生体エネルギー変換 / ヌクレオチド結合 / 脱共役 / 酸化的リン酸化 / 調節 / ATP / 酵素 / 構造機能相関 / アロステリック |
Outline of Final Research Achievements |
(1) Biochemical analysis of Bacillus subtilis F1-ATPase (BF1) revealed that BF1 is strongly inhibited by MgADP inhibition and the "inhibitory" epsilon subunit acts as an activator in BF1. (2) It was revealed that the strong MgADP inhibition of BF1 is not due to the low ATP binding affinity of noncatalytic sites. (3) Overexpression system of BFoF1 was successfully constructed. (4) Bacillus subtilis strain containing an ATP-sensor protein (ATeam) was constructed. (5) Physiological conditions where the regulation of BFoF1 by the epsilon subunit is prominent were revealed.
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Report
(6 results)
Research Products
(15 results)