ER redox regulation by ER GSH transporter

• Project/Area Number: 23770158
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Kyoto University
• Principal Investigator: HOSEKI Jun 京都大学, 生理化学研究ユニット, 特定准教授 (40423058)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: レドックス / 小胞体 / グルタチオン / トランスポーター

Research Abstract

Glutathione is required for ER redox regulation. GSH is synthesized in cytosol while the mechanism by which GSH is transported into the ER is still unknown. We showed that ER membrane proteins are necessary for GSH transport into the ER and identified inhibitors for GSH transport into the ER by a sensitive detection system using roGFP for GSH transport into the ER.

Research Products

• [Journal Article] fluorescence resonance energy transfer (FRET)-based redox sensor reveals physiological role of thioredoxin in the yeast Saccharomyces cerevisiae (2013)
  • Author(s): M. Oku, J. Hoseki, Y. Ichiki, and Y. Sakai.A
  • Journal Title: FEBS Lett. Volume: 587 Issue: 6 Pages: 793-798
  • DOI: 10.1016/j.febslet.2013.02.003
  • NAID: 120005244260
  • Peer Reviewed
• [Journal Article] Structural basis of an ERAD pathway mediated by the ER-resident protein disulfide reductase ERdj5 (2011)
  • Author(s): M. Hagiwara, K. Maegawa, M. Suzuki, R. Ushioda, K. Araki, Y. Matsumoto, J. Hoseki, K. Nagata and K. Inaba
  • Journal Title: Mol. Cell Volume: 41(4) Issue: 4 Pages: 432-444
  • DOI: 10.1016/j.molcel.2011.01.021
  • NAID: 120003836694
  • Peer Reviewed
• [Presentation] 酵母Saccharomyces ceruvisiaeにおけるペルオキシソーム内レドックス制御因子の解析 (2013)
  • Author(s): 島並祐子、奥 公秀、寳関 淳、阪井康能
  • Organizer: 日本農芸化学会関西支部第478回講演会
  • Place of Presentation: 京都市
  • Year and Date: 2013-02-02
• [Presentation] Involvement in mitochondrial function by antioxidants protects cellular oxidation caused by proteasome inhibiton (2013)
  • Author(s): Maharjan Sunita、寳関 淳、奥 公秀、阪井康能
  • Organizer: 日本農芸化学会関西支部第478回講演会
  • Place of Presentation: 京都市
  • Year and Date: 2013-02-02
• [Presentation] レドックス制御とタンパク質品質管理 (2012)
  • Author(s): 寳関 淳
  • Organizer: 京都大学学際融合教育研究推進センター 生理化学研究ユニット 第2回シンポジウム
  • Place of Presentation: 京都市
  • Year and Date: 2012-06-13
• [Presentation] 細胞内レドックス状態の可視化とこれを利用したレドックスモジュレーターの探索 (2012)
  • Author(s): 寳関 淳、阪井康能
  • Organizer: 疾病予防・食品機能開発評価システムに関するフォーラム
  • Place of Presentation: 京都市
  • Year and Date: 2012-03-13
• [Presentation] Biochemical characterisation of Pfj2, a Plasmodium falciparum heat shock protein 40 chaperone potentially involved in protein quality control in the endoplasmic reticulum (2012)
  • Author(s): Omolola Afolayan、Eva-Rachele Pesce、寳関 淳、平山尚志郎、永田和宏、Caroline Knox、Gregory L. Blatch
  • Organizer: South African Society for Biochemistry and Molecular Biology/Federation of African Societies of Biochemistry and Molecular Biology SASBMB/FASBMB Congress 2012
  • Place of Presentation: South Africa
• [Presentation] Glycosylation-Independent ERAD pathway serves as a backup system under ER stress (2012)
  • Author(s): 潮田 亮、寳関 淳、永田和宏
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会ワークショップ
  • Place of Presentation: 神戸市
• [Presentation] Reduction mechanism of a disulfide reductase, ERdj5 in the oxidative redox environment of the ER (2012)
  • Author(s): 寳関 淳、阪井康能、永田和宏
  • Organizer: Gordon Research Conferences: Thiol-Based Redox Regulation& Signaling
  • Place of Presentation: USA
• [Presentation] Glycosylation-independent ERAD pathway serves as a backup system under ER stress (2012)
  • Author(s): 潮田 亮、寳関 淳、永田和宏
  • Organizer: EMBO/EMBL Symposium "Quality Control-From Molecules to Organelles"
  • Place of Presentation: Germany
• [Presentation] Reduction mechanism of a disulfide reductase, ERdj5 in the oxidative redox environment of the ER. (2012)
  • Author(s): Jun Hoseki, Yasuyoshi Sakai, Kazuhiro Nagata
  • Organizer: Gordon Research Conferences: Thiol-Based Redox Regulation & Signaling
  • Place of Presentation: Lewiston, ME (USA)
• [Presentation] Glycosylation-independent ERAD pathway serves as a backup system under ER stress (2012)
  • Author(s): Ryo Ushioda, Jun Hoseki, Kazuhiro Nagata
  • Organizer: EMBO/EMBL Symposium “Quality Control-From Molecules to Organelles”
  • Place of Presentation: Heiderberg(Germany)
• [Presentation] Biochemical characterisation of Pfj2, a Plasmodium falciparum heat shock protein 40 chaperone potentially involved in protein quality control in the endoplasmic reticulum. (2012)
  • Author(s): Omolola Afolayan, Eva-Rachele Pesce, Jun Hoseki, Shoshiro Hirayama, Kazuhiro Nagata, Caroline Knox and Gregory L. Blatch
  • Organizer: SASBMB/FASBMB Congress 2012
  • Place of Presentation: Drakensberg(South Africa)
• [Presentation] ジスルフィド還元酵素ERdj5 による小胞体関連分解のレドックス制御 (2011)
  • Author(s): 寳関 淳
  • Organizer: 第84回日本生化学会大会シンポジウム「レドックス反応システムによる細胞制御機構」
  • Place of Presentation: 京都市
  • Year and Date: 2011-09-21
• [Presentation] 糖たんぱく質ERADにおけるERdj5の役割/The role of ERdj5 in glycoprotein ERAD pathway (2011)
  • Author(s): 萩原誠智、前川憲一、鈴木 守、潮田 亮、新木和孝、松本悠史、寳関 淳、永田和宏、稲葉謙次
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 札幌市
• [Presentation] Non-glycoprotein ERAD pathway serves as a backup system under ER stress (2011)
  • Author(s): 潮田 亮、寳関 淳、永田和宏
  • Organizer: Cold Spring Harbor Asia Conference, "Protein Homeostasis in Health & Disease"
  • Place of Presentation: China
• [Presentation] 糖たんぱく質ERADにおける ERdj5の役割/The role of ERdj5 in glycoprotein ERAD pathway. (2011)
  • Author(s): 萩原誠智、前川憲一、鈴木守、潮田亮、新木和孝、松本悠史、 寳関淳、永田和宏、稲葉謙次
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学(札幌市)
• [Presentation] Non-glycoprotein ERAD pathway serves as a backup system under ER stress. (2011)
  • Author(s): Ryo Ushioda, Jun Hoseki and Kazuhiro Nagata
  • Organizer: Cold Spring Harbor Asia Conference, "Protein Homeostasis in Health & Disease"
  • Place of Presentation: Suzhou(China)
• [Book] 酵母のレドックス認識機構を応用した新しいセンサータンパク質の開発 (2013)
  • Author(s): 寳関 淳、奥 公秀、阪井康能
  • Publisher: FRAGRANCE JOURNAL
• [Book] レドックス異常を回復する化合物レドックスモジュレーターの探索:Redoxfluorの創薬への利用 (2012)
  • Author(s): 阪井康能、寳関 淳、奥 公秀
  • Publisher: 遺伝子医学MOOK
• [Remarks] 研究室ホームページ
  • URL: http://www.seigyo.kais.kyoto-u.ac.jp