| Project/Area Number |
23770159
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Kyoto Sangyo University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KUROSAKA Akira 京都産業大学, 総合生命科学部, 教授 (90186536)
NAKAMURA Naosuke 京都産業大学, 総合生命科学部, 講師 (30424964)
ITOH Nobuyuki 京都大学, 薬学研究科, 教授 (10110610)
MIYAKE Ayumi 京都大学, 薬学研究科, 講師 (40346044)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 糖鎖 / 神経発生 / ゼブラフィッシュ / ムチン / 網膜 / 糖転移酵素 |
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| Research Abstract |
We cloned and identified 18 genes encoding ppGalNAcT from zebrafish cDNA library, corresponding to 20 isozymes in human genome. Then, we detected their mRNAs in the embryos during early developmental stages, and found that each of members has characteristic expression patterns mainly in eye, brain and skeletal muscle. Suppression of ppGalNAcT17 and T18 using antisense RNA led to developmental defects in the central nervous system and tail, respectively.
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