Epigenetic regulation of brain-specific O-mannose glycans

• Project/Area Number: 23770163
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: KIZUKA Yasuhiko 独立行政法人理化学研究所, 疾患糖鎖研究チーム, 基礎科学特別研究員 (20564743)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 糖鎖生物学 / 糖転移酵素 / エピジェネティクス / GnT-IX / O-GlcNAc / HDAC / 国際研究者交流 / 国際情報交換 / 国際情報交流

Research Abstract

Aim of this study is to unveil how glycans are expressed in tissue-specific manners. In detail, we focused on a brain-specific glycan, branched O-mannose glycan. As a novel approach, concept and technique of epigenetics were adopted. As a result, expression of GnT-IX that is essential for biosynthesis of O-brain-specific mannose glycans, is epigenetically regulated by post translational modifications of histone.

Research Products

• [Journal Article] Epigenetic regulation of a brain-specific glycosyltransferase N-acetylglucosaminyltransferase-IX (GnT-IX) by specific chromatin modifiers (2014)
  • Author(s): Kizuka Y, Kitazume S, Okahara K, Villagra A, Sotomayor EM, Taniguchi N
  • Journal Title: J Biol Chem. (Epub 2014 Mar10.) Volume: 289(16) Issue: 16 Pages: 11253-61
  • DOI: 10.1074/jbc.m114.554311
  • Peer Reviewed
• [Journal Article] Regulated expression and neural functions of human natural killer-1 (HNK-1) carbohydrate. (2012)
  • Author(s): Y. Kizuka and S. Oka.
  • Journal Title: Cell Mol. Life Sci Volume: 69(24) Issue: 24 Pages: 4135-47
  • DOI: 10.1007/s00018-012-1036-z
  • NAID: 120005122349
  • Peer Reviewed
• [Journal Article] エピゲノムによる糖鎖生合成遺伝子GnT-IXの脳特異的な発現制御機構 (2012)
  • Author(s): 木塚 康彦、北爪 しのぶ、谷口 直之
  • Journal Title: THE LUNG perspectives Volume: 20 Pages: 75-80
• [Journal Article] Brain-specific expression of N-acetylglucosaminyltransferase IX (GnT-IX) is regulated by epigenetic histone modifications (2011)
  • Author(s): Y.Kizuka, S.Kitazume, M.Yoshida, N.Taniguchi
  • Journal Title: J.Biol.Chem. Volume: 286 Issue: 36 Pages: 31875-31884
  • DOI: 10.1074/jbc.m111.251173
  • Peer Reviewed
• [Presentation] New epigenetic factors for brain-specific glycosyltransferase gene expression. (2013)
  • Author(s): Yasuhiko Kizuka, Shinobu Kitazume, Naoyuki Taniguchi.
  • Organizer: 2013 Society for Glycobiology Annual Meeting.
  • Place of Presentation: Tampa(USA).
  • Year and Date: 2013-11-18
• [Presentation] 新規エピゲノム因子による脳特異的糖転移酵素GnT-IXの遺伝子発現制御. (2013)
  • Author(s): 木塚康彦,北爪しのぶ,谷口直之
  • Organizer: GlycoTOKYO2013シンポジウム.
  • Place of Presentation: 成蹊大学(東京)
  • Year and Date: 2013-10-19
• [Presentation] 脳特異的糖転移酵素GnT-IXの遺伝子発現を制御する新規エピゲノム因子の探索. (2013)
  • Author(s): 木塚康彦,北爪しのぶ,谷口直之
  • Organizer: 第32回日本糖質学会年会.
  • Place of Presentation: 大阪
  • Year and Date: 2013-08-07
• [Presentation] New epigenetic factors for brain-specific glycosyltransferase gene expression. (2013)
  • Author(s): Yasuhiko Kizuka, Shinobu Kitazume, Naoyuki Taniguchi.
  • Organizer: The 3^<rd> Austria/Japan Seminar on Comparative and Developmental Glycobiology.
  • Place of Presentation: 理化学研究所(和光)
  • Year and Date: 2013-07-02
• [Presentation] Epigenetic regulation of glycosyltransferase gene expression. (2013)
  • Author(s): Yasuhiko Kizuka, Shinobu Kitazume, Naoyuki Taniguchi.
  • Organizer: XXII International Symposium on Glybobonjugates.
  • Place of Presentation: Dalian(China)
  • Year and Date: 2013-06-24
• [Presentation] Gene expression of glycosyltransferase is regulated by selective epigenetic factors. (2013)
  • Author(s): 木塚康彦、北爪しのぶ、谷口直之
  • Organizer: RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, The second symposium.
  • Place of Presentation: 理化学研究所(和光)
  • Year and Date: 2013-04-17
• [Presentation] Gene expression of brain specific glycosyltransferases and their epigenetic regulation (2012)
  • Author(s): Yasuhiko Kizuka, Kyohei Okahara, Shinobu Kitazume, Naoyuki Taniguchi.
  • Organizer: Joint Meeting of the Society for Glycobiology and American Society for Matrix Biology
  • Place of Presentation: San Diego(USA)
• [Presentation] エピジェネティクスによる脳特異的な糖転移酵素の発現制御メカニズム. (2012)
  • Author(s): 木塚康彦、岡原京平、北爪しのぶ、谷口直之
  • Organizer: 第31回日本糖質学会年会
  • Place of Presentation: 鹿児島
• [Presentation] Brain specific regulation of GnT-IX expression by epigenetic mechanism (2012)
  • Author(s): Yasuhiko Kizuka, Shinobu Kitazume, Minoru Yoshida, Naoyuki Taniguchi.
  • Organizer: 2^<nd> RIKEN-SNU symposium.
  • Place of Presentation: Nami island (Korea)
• [Presentation] Brain specific regulation of GnT-IX expression by epigenetic mechanism (2012)
  • Author(s): Yasuhiko Kizuka, Shinobu Kitazume, Minoru Yoshida, Naoyuki Taniguchi
  • Organizer: 2nd RIKEN-SNU Symposium
  • Place of Presentation: Nami island
• [Presentation] エピジェネティクスによる脳特異的な糖転移酵素の発現制御メカニズム (2012)
  • Author(s): 木塚康彦、岡原京平、北爪しのぶ、谷口直之
  • Organizer: 第31回日本糖質学会年会
  • Place of Presentation: 鹿児島
• [Presentation] Gene expression of brain specific glycosyltransferases and their epigenetic regulation (2012)
  • Author(s): Yasuhiko Kizuka, Kyohei Okahara, Shinobu Kitazume and Naoyuki Taniguchi
  • Organizer: Joint Meeting of the Society for Glycobiology and American Society for Matrix Biology
  • Place of Presentation: San Diego
• [Presentation] 脳特異的糖転移酵素GnT-IX遺伝子のエピジェネティックな発現制御 (2011)
  • Author(s): 木塚康彦,北爪しのぶ,谷口直之
  • Organizer: GlycoTOKYO 2011シンポジウム
  • Place of Presentation: 理化学研究所(和光)
  • Year and Date: 2011-12-09
• [Presentation] ヒストンコードにより制御されるGnT-IX遺伝子の脳特異的発現機構 (2011)
  • Author(s): 木塚康彦、北爪しのぶ、吉田稔、谷口直之
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2011-09-22
• [Presentation] Regulation of brain-specific GnT-IX (Vb) expression by neural histone-code (2011)
  • Author(s): Yasuhiko Kizuka, Shinobu Kitazume, Minoru Yoshida, Naoyuki Taniguchi.
  • Organizer: 第31回内藤コンファレンス
  • Place of Presentation: シャトレーゼ・ガトーキングダムサッポロ(札幌)
  • Year and Date: 2011-09-14
• [Presentation] Histone-code regulates brain specific expression of N-acetylglucosaminyltransferase-IX (GnT-IX) (2011)
  • Author(s): Yasuhiko Kizuka, Shinobu Kitazume, Minoru Yoshida, Naoyuki Taniguchi.
  • Organizer: Glyco21, 21st international symposium on glycoconjugates
  • Place of Presentation: ウィーン大学(Austria)
  • Year and Date: 2011-08-24
• [Presentation] Histone-code regulates brain specific expression of N-acetylglucosaminyltransferase-IX (GnT-IX) (2011)
  • Author(s): Yasuhiko Kizuka, Shinobu Kitazume, Minoru Yoshida, Naoyuki Taniguchi
  • Organizer: Glyco21, 21st international symposium on glycoconjugates
  • Place of Presentation: ウィーン大学(オーストリア)
• [Presentation] Regulation of brain-specific GnT-IX (Vb) expression by neural histone-code (2011)
  • Author(s): Yasuhiko Kizuka, Shinobu Kitazume, Minoru Yoshida, Naoyuki Taniguchi
  • Organizer: 第31回内藤コンファレンス
  • Place of Presentation: シャトレーゼ・ガトーキングダムサッポロ（北海道）
• [Presentation] ヒストンコードにより制御されるGnT-IX遺伝子の脳特異的発現機構 (2011)
  • Author(s): 木塚康彦、北爪しのぶ、吉田稔、谷口直之
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都府)
• [Presentation] 脳特異的糖転移酵素GnT-IX遺伝子のエピジェネティックな発現制御 (2011)
  • Author(s): 木塚康彦, 北爪しのぶ, 谷口直之
  • Organizer: GlycoTOKYO 2011 シンポジウム
  • Place of Presentation: 理化学研究所(埼玉県)
• [Presentation] Gene expression of glycosyltransferase is regulated by selective epigenetic factors
  • Author(s): 木塚康彦、北爪しのぶ、谷口直之
  • Organizer: RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, The second symposium
  • Place of Presentation: 理化学研究所（和光）
• [Presentation] Epigenetic regulation of glycosyltransferase gene expression
  • Author(s): 木塚康彦、北爪しのぶ、谷口直之
  • Organizer: XXII International Symposium on Glycoconjugates
  • Place of Presentation: 大連（中国）
• [Presentation] 脳特異的糖転移酵素GnT-IXの遺伝子発現を制御する新規エピゲノム因子の探索
  • Author(s): 木塚康彦、北爪しのぶ、谷口直之
  • Organizer: 第32回日本糖質学会年会
  • Place of Presentation: 大阪
• [Presentation] New epigenetic factors for brain-specific glycosyltransferase gene expression
  • Author(s): 木塚康彦、北爪しのぶ、谷口直之
  • Organizer: The 3rd Austria/Japan Seminar on Comparative and Developmental Glycobiology
  • Place of Presentation: 理化学研究所（和光）
• [Presentation] 新規エピゲノム因子による脳特異的糖転移酵素GnT-IXの遺伝子発現制御
  • Author(s): 木塚康彦、北爪しのぶ、谷口直之
  • Organizer: GlycoTOKYO2013シンポジウム
  • Place of Presentation: 東京（成蹊大学
• [Presentation] New epigenetic factors for brain-specific glycosyltransferase gene expression
  • Author(s): 木塚康彦、北爪しのぶ、谷口直之
  • Organizer: 2013 Society for Glycobiology Annual Meeting
  • Place of Presentation: タンパ（アメリカ）
• [Remarks] 理化学研究所のプレスリリース<URL>
  • URL: http://www.riken.jp/pr/press/2011/20110725/
• [Remarks]
  • URL: http://www.riken.jp/pr/press/2014/20140312_1/
• [Remarks] 理化学研究所のResearch Highlights
  • URL: http://www.riken.jp/en/research/rikenresearch/highlights/6760/
• [Remarks] 糖鎖遺伝子「GnT-IX」が脳だけに発現する新たな仕組みを解明
  • URL: http://www.riken.jp/pr/press/2014/20140312_1/
• [Remarks] RIKEN RESERACH
  • URL: http://www.rikenresearch.riken.jp/eng/research/6760
• [Remarks] 糖転移酵素「GnT-IX」が脳だけに存在する仕組みを解明
  • URL: http://www.riken.jp/pr/press/2011/20110725/