Regulation of glycosphingolipids biosynthesis by multi-spanning transmembrane protein family TMBIMs and their molecular machinery
Project/Area Number |
23770164
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Functional biochemistry
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Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
YAMAJI Toshiyuki 国立感染症研究所, 細胞化学部, 主任研究官 (50332309)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | スフィンゴ糖脂質 / TMBIMファミリー / FAIM2 / 糖脂質 / 膜タンパク / 代謝 / 遺伝子編集法 / 糖鎖 |
Research Abstract |
In this study, I examined new functions of FAIM2, a member of TMBIM family, based on my previous report that TMBIM family members reduced a glycosphingolipid, Gb3. Consequently, I found the following points: (1) FAIM2 interacted with NEDD4 E3 ligase family proteins through the N-terminal cytosolic domain of FAIM2; (2) an aspartate residue that is commonly observed in the multi-spanning membrane domains of TMBIM family was critical for two activities of FAIM2, the inhibitory activity to FAS-mediated apoptosis and Gb3-reducing activity. On the other hand, as a new tool to analyze glycolipid metabolism, I established a series of HeLa cell mutants deficient in sphingolipid-related genes including glucosylceramide synthase using TALENs.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Genetic variation of human papillomavirus type 16 in individual clinical specimens revealed by deep sequencing2013
Author(s)
Kukimoto I, Maehama T, Sekizuka T, Ogasawara Y, Kondo K, Kusumoto-Matsuo R, Mori S, Ishii Y, Takeuchi T, Yamaji T, Takeuchi F, Hanada K, Kuroda M.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Colonic epithelial cells express specific ligands for mucosal macrophage immunosuppressive receptors siglec-7 and -92012
Author(s)
Miyazaki K, Sakuma K, Kawamura YI, Izawa M, Ohmori K, Mitsuki M, Yamaji T, Hashimoto Y, Suzuki A, Saito Y, Dohi T, Kannagi R.
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Journal Title
J Immunol
Volume: 188
Pages: 4690-700
Related Report
Peer Reviewed
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[Journal Article] Colonic epithelial cells express specific ligands for mucosal macrophage immunosuppressive receptors, siglec-7 and-92012
Author(s)
Miyazaki, K., Sakuma, K., Kawamura, Y. I., Izawa, M., Ohmori, K., Mitsuki, M., Yamaji, T., Hashimoto, Y., Suzuki, A., Saito, Y., Dohi, T., and Kannagi, R.
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Journal Title
J. Immunol
Volume: 188
Issue: 9
Pages: 4690-4700
DOI
Related Report
Peer Reviewed
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[Presentation] Biding of Siglec-9 to the counter-receptors on cancer cells regulates adhesion dynamics via protease-mediated degradation of focal adhesion kinase (FAK)2013
Author(s)
Yilihamujiang, S., Hashimoto, N., Matsumoto, Y., Hashimoto, Y., Yamaji, T., Furukawa, K., Furukawa, K.
Organizer
第86回日本生化学会大会
Place of Presentation
横浜
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