| Project/Area Number |
23770170
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ODA Toshiyuki 東京大学, 大学院・医学系研究科, 助教 (20569090)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 構造生物学 / 鞭毛・線毛 / ダイニン / 分子モーター / クライオ電子顕微鏡 / 鞭毛・繊毛 / 三次元構造解析 / 微小管 / 三次元再構成 / 鞭毛 / 細胞生物学 |
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| Research Abstract |
In flagella, outer dynein arm (ODA) and inner dynein arm (IDA) play distinct rolesin generating beating motion. However, functional communications between the two dyneinshave not been investigated. Here, we demonstrated by cryo-electron microscopy and chemicalcrosslinking that intermediate chain 2 (IC2) of ODA interacts with the dynein regulatorycomplex in the axoneme and constitutes part of the outer-inner dynein (OID) linker.Furthermore, we identified IC2 as a functional hub between ODA and IDA based on thephenotypes of Chlamydomonas mutants expressing biotinylation-tagged IC2. The flagella ofthe IC2 mutant showed activated microtubule sliding and enhanced ATPase activities ofODA, as well as an altered waveform, indicating attenuated IDA activity. We concluded thatthe OID linker controls both ODA and IDA and regulates flagellar beating.
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