Functional and single-molecular analysis of neutrophil in vivo by non-invasive mesurements
Project/Area Number |
23770171
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Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Biophysics
|
Research Institution | The University of Tokyo |
Principal Investigator |
KIKUSHIMA Kenji 東京大学, 大学院・理学系研究科, 研究員 (50569142)
|
Research Collaborator |
MIKAMI Takashi 東京大学, 大学院・理学系研究科, 大学院生
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | バイオイメージング / 生体内メージング / 小胞輸送 / 非侵襲イメージング / in vivo / 好中球 |
Research Abstract |
I created a fluorescent dye enough bright for the non-invasive imaging of mice using quantum dots. I constructed a high-contrast imaging system and succeeded in non-invasive observation of neutrophil labeled by the quantum dots in mouse ear. Fast vesicle movements inside the neutrophils were also able to be observed and were analyzed. I constructed a new microscope that enables us to measure momentum of the particle that has an undetermined form. Using the system, I measured that vesicles in Hela cell were transported with 2-38 pN force.
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Report
(3 results)
Research Products
(15 results)