Statistical analysis of flagella beating by 3-D tracking microscopy

Research Project

Project/Area Number	23770172
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Biophysics
Research Institution	Kyoto University
Principal Investigator	YAMANO Takashi 京都大学, 大学院・生命科学研究科, 助教 (70570167)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords	運動・輸送 / クラミドモナス / 三次元トラッキング顕微鏡 / トラッキング顕微鏡
Research Abstract	We developed 3D-tracking microscopy by a new image processing algorithm to achieve high-speed autofocusing of Chlamydomonas reinhardtii, a motile green alga. Using this algorithm and high-speed visual feedback, we could pursue a single Chlamydomonas cell to keep it at the center of the field and depth of view, and visualize the two flagella continuously along with tracking it at high spatiotemporal resolution.