Development of novel type of flat lipid membrane for observation of complex formation process and structural analysis of divisome
| Project/Area Number |
23770173
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 細胞膜 / 膜タンパク質の複合体 / 平面脂質膜 / 膜タンパク質 / 膜分裂 / 細胞膜分裂 / 膜蛋白質 |
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| Research Abstract |
Many of membrane proteins are recruited at cell division site and drive the E. coli cell division. The membrane proteins interact with each other and forms huge protein complex named divisome. At present, fundamental mechanism of divisome formation and structure of the complex on cell membrane has not been clear. In this study, we developed a new model membrane, which can analyze a structure and functions of huge membrane proteins complex, and tried to reveal molecular mechanism of E. coli cell division. We succeeded in flat lipid membrane at liquid-air interface using E. coli extracted lipid. We prepared some GFP fusion membrane proteins, which drive cell division, and reconstituted into the model membrane. At the end of this study period, we could not find membrane protein into the model membrane. Recently, we developed a new method for preparation of cell membrane sheet derived from cultured cell. We will keep trying to analyze membrane protein complex by using the model membrane.
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Report
(4 results)
Research Products
(16 results)
