• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of novel type of flat lipid membrane for observation of complex formation process and structural analysis of divisome

Research Project

Project/Area Number 23770173
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionYamagata University

Principal Investigator

OKUNO Takashi  山形大学, 理学部, 准教授 (80411031)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords細胞膜 / 膜タンパク質の複合体 / 平面脂質膜 / 膜タンパク質 / 膜分裂 / 細胞膜分裂 / 膜蛋白質
Research Abstract

Many of membrane proteins are recruited at cell division site and drive the E. coli cell division. The membrane proteins interact with each other and forms huge protein complex named divisome. At present, fundamental mechanism of divisome formation and structure of the complex on cell membrane has not been clear. In this study, we developed a new model membrane, which can analyze a structure and functions of huge membrane proteins complex, and tried to reveal molecular mechanism of E. coli cell division. We succeeded in flat lipid membrane at liquid-air interface using E. coli extracted lipid. We prepared some GFP fusion membrane proteins, which drive cell division, and reconstituted into the model membrane. At the end of this study period, we could not find membrane protein into the model membrane. Recently, we developed a new method for preparation of cell membrane sheet derived from cultured cell. We will keep trying to analyze membrane protein complex by using the model membrane.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (16 results)

All 2013 2012 Other

All Presentation (11 results) Book (2 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Presentation] Development of a new method for preparation of cell membrane flat sheet on glass surface2013

    • Author(s)
      南祐太,飯沼博章,櫻井敏彦,奥野貴士
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of method for preparation of giant plasma membrane vesicles using surfactants2013

    • Author(s)
      飯沼博章,南祐太,櫻井敏彦,奥野貴士
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] GPMVs由来細胞膜シートの物性評価と微細加工基板によるマニピレーション技術2013

    • Author(s)
      南祐太,飯沼博章,櫻井敏彦,奥野貴士
    • Organizer
      細胞を創る研究会6.0
    • Place of Presentation
      鶴岡
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胞膜を試験管に取り出す方法2013

    • Author(s)
      川畑智美,南祐太,櫻井敏彦,奥野貴士
    • Organizer
      細胞を創る研究会6.0
    • Place of Presentation
      鶴岡
    • Related Report
      2013 Final Research Report
  • [Presentation] 膜タンパク質解析のためのモデル膜開 発:細胞膜を切出してガラス基板に展開した平面膜構造体2013

    • Author(s)
      南祐太,飯沼博章,小野寺美紀,櫻井敏彦,奥野貴士
    • Organizer
      日本膜学会年会
    • Place of Presentation
      早稲田大学 西早稲田キャンパス(東京)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of a new method for preparation of cell membrane flat sheet on glass surface2013

    • Author(s)
      南祐太,飯沼博章,櫻井敏彦,奥野貴士
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of method for preparation of giant plasma membrane vesicles using surfactants2013

    • Author(s)
      飯沼博章,南祐太,櫻井敏彦,奥野貴士
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] GPMVs由来細胞膜シートの物性評価と微細加工基板によるマニピレーション技術2013

    • Author(s)
      南祐太,飯沼博章,櫻井敏彦,奥野貴士
    • Organizer
      細胞を創る研究会6.0
    • Place of Presentation
      慶應義塾大学鶴岡タウンキャンパス(鶴岡)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細胞膜を試験管に取り出す方法2013

    • Author(s)
      川畑智美,南祐太,櫻井敏彦,奥野貴士
    • Organizer
      細胞を創る研究会6.0
    • Place of Presentation
      慶應義塾大学鶴岡タウンキャンパス(鶴岡)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ベシクル状に切り出した細胞膜を基板上に展開する研究2012

    • Author(s)
      南祐太,飯沼博章,櫻井敏彦,奥野貴士
    • Organizer
      細胞を創る研究会5.0
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] 界面活性物質を用いて細胞膜をベシクル状に切り出す研究2012

    • Author(s)
      飯沼博章,南祐太,菅原有希,上野雅晴,櫻井敏彦,奥野貴士
    • Organizer
      細胞を創る研究会5.0
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Book] FtsH Protease-Mediated Regulation of Various Cellular Functions2013

    • Author(s)
      Okuno T., Ogura T.
    • Publisher
      Subcell Biochem
    • Related Report
      2013 Final Research Report
  • [Book] Regulated proteolysis in microorganisms.2013

    • Author(s)
      Okuno, T., and Ogura, T.
    • Total Pages
      8
    • Publisher
      FtsH protease-mediated regulation of various cellular functions.
    • Related Report
      2012 Research-status Report
  • [Remarks] 山形大学理学部物質生命化学科 奥野研究室

    • URL

      http://www-kschem0.kj.yamagata-u.ac.jp/%7Eokuno/indexk.html

    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 動物細胞から細胞膜を切り出す方法、及び切り出された細胞膜2013

    • Inventor(s)
      野貴士、南祐太、小野寺美紀
    • Industrial Property Rights Holder
      国立大学法人山形大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-11-01
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 動物細胞から細胞膜を切り出す方法、及び切り出された細胞膜2013

    • Inventor(s)
      奥野貴士、南祐太、小野寺美紀
    • Industrial Property Rights Holder
      国立大学法人山形大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-228766
    • Filing Date
      2013-11-01
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi