| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
For the purpose of revealing structure of DNA hydration including structural fluctuation, neutron and X-ray crystallographic studies were carried out. (1) Deuterated Z-DNA d(CGCGCG) could be produced. (2) Large single crystals of Z-DNA d(CGCGCG) were obtained from H2O solution, and TOF neutron experiment was performed using pulsed neutron source. Neutron diffraction spots were detected up to 1.6 -1.8 Angstrom. Protocol for refinements was tested using neutron data sets of protein crystals. X-ray crystallographic analyses were also carried out complementarily, as the result, Z-DNA structures at high and low concentrations of metal salts were determined. Each of these structures had specific features, suggesting that these crystals would be dominant candidates for neutron crystallographic studies.
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