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Tracking of the bacterial flagellar rotation using single fluorescent microscopy

Research Project

Project/Area Number 23770183
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionHosei University

Principal Investigator

SOWA Yoshiyuki  法政大学, 生命科学部, 講師 (10519440)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords細菌べん毛モーター / 分子モーター
Research Abstract

The bacterial flagellar motor is a reversible rotary nano-machine, which is powered by ion flux across the cell membrane. The motor has several interesting features as fast rotation, regulation system of torque generation and so on. Their rotation, however, have been measured via flagellar filaments extend from cell body, rather than via the motor basal body directly. To track its rotation directly at molecular level, we have developed single molecule fluorescent microscopy and methods to label the motor basal body.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (35 results)

All 2013 2012 2011 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (29 results) Book (2 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Microscopic Analysis of Bacterial Motility at High Pressure2012

    • Author(s)
      Nishiyama M., Sowa Y.
    • Journal Title

      Biophysical Journal

      Volume: 102 Issue: 8 Pages: 1872-1880

    • DOI

      10.1016/j.bpj.2012.03.033

    • NAID

      120004057183

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Dynamicconformationalchangesofflagellarfilamentobservedbyhigh-pressuremicroscopy2013

    • Author(s)
      Nishiyama,M.&Sowa,Y.
    • Organizer
      57thAnnualMeetingofBiophysicalSociety
    • Place of Presentation
      Philadelphia(アメリカ)
    • Year and Date
      2013-02-06
    • Related Report
      2012 Final Research Report
  • [Presentation] 液体ハンドリングのみによって行う微生物ゲノムの連続編集2013

    • Author(s)
      冨永将大,嶋村陽,曽和義幸,川岸郁朗,斎藤恭一,梅野太輔
    • Organizer
      第6回日本ゲノム微生物学会年会
    • Place of Presentation
      長浜バイオ大学(滋賀県)
    • Related Report
      2012 Final Research Report
  • [Presentation] 液体ハンドリングのみによって行う微生物ゲノムの連続編集2013

    • Author(s)
      冨永将大
    • Organizer
      第6回日本ゲノム微生物学会年会
    • Place of Presentation
      長浜バイオ大学 (滋賀県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Bacterial motility measured by a high-pressure microscope2012

    • Author(s)
      Nishiyama, M. & Sowa, Y.
    • Organizer
      7th International Conference on High Pressure Bioscience and Biotechnology (HPBB2012)
    • Place of Presentation
      ピアザ淡海 (滋 賀県)
    • Year and Date
      2012-10-31
    • Related Report
      2012 Final Research Report
  • [Presentation] Exploring the mechanism underlying sensing of the repellent Ni2+ by the aspartate chemoreceptor Tar of Escherichia coli2012

    • Author(s)
      Inui, T., Tajima, H., Sowa, Y. & Kawagishi, I.
    • Organizer
      第50回生物物理学会年 会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Year and Date
      2012-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Control of bacterial flagellar rotation via crosstalk from a non-cognate histidine kinase to the response regulator CheY2012

    • Author(s)
      Umemura, T., Hara, C., Sowa, Y. & Kawagishi, I.
    • Organizer
      第50回生物物理 学会年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Year and Date
      2012-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Intracellular dynamics of xenobiotic efflu2012

    • Author(s)
      Yamamoto, K., Inaba, T., Sowa, Y. & Kawagishi, I.
    • Organizer
      x proteins in Escherichia coli. 第50回生物物理学会年会
    • Place of Presentation
      名古屋大 学 (愛知県)
    • Year and Date
      2012-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Imaging of fluorescently-tagged motor components of bacterial flagella2012

    • Author(s)
      Sowa, Y. & Che, Y-S.
    • Organizer
      第50回生物物理学会 年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Year and Date
      2012-09-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Rotational measurement of Na+-driven chimeric flagellar motor with tandem PomA2012

    • Author(s)
      Che, Y-S & Sowa, Y.
    • Organizer
      第50回生物物理学会年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Year and Date
      2012-09-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Dynamic conformational changes of flagellar filament observed by high-pressure microscopy2012

    • Author(s)
      Nishiyama, M. & Sowa, Y.
    • Organizer
      第50回生物物理学会年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Year and Date
      2012-09-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Sodium dynamics of the bacterial flagellar motor2012

    • Author(s)
      Lo, C-J., Sowa, Y., Pilizota, T. & Berry, R.
    • Organizer
      第50回生物物理学会年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Year and Date
      2012-09-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Microscopic analysis of bacterial motility at high pressure (Platform)2012

    • Author(s)
      M. Nishiyama and Y. Sowa.
    • Organizer
      56th Annual Meeting of Biophysical Society
    • Place of Presentation
      Boston アメリカ
    • Related Report
      2012 Final Research Report
  • [Presentation] A new setup for tracking of bacterial flagellar rotation in3-dimensionally2012

    • Author(s)
      Sowa, Y.
    • Organizer
      2012 Gordon ResearchConference on Sensory Transduction in Microorganisms
    • Place of Presentation
      Ventura (アメリカ)
    • Related Report
      2012 Final Research Report
  • [Presentation] 高圧力下にあるバク テリア遊泳運動の阻害機構2012

    • Author(s)
      西山雅祥,曽和義幸
    • Organizer
      2012 年 生体運 動研究合同班会議
    • Place of Presentation
      筑波大学 (茨城県)
    • Related Report
      2012 Final Research Report
  • [Presentation] Three-dimensinal tracking of bacterial flagellar rotation2011

    • Author(s)
      Sowa, Y.
    • Organizer
      日本生物物理学会
    • Place of Presentation
      兵庫県立大学 (兵庫県)
    • Year and Date
      2011-09-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸菌走 化性応答のビデオ解析2011

    • Author(s)
      藤畑将理, 高橋浩一, 曽和義幸.
    • Organizer
      21 世紀大腸菌研究 会
    • Place of Presentation
      ホテル木曽路 (長野県)
    • Year and Date
      2011-05-19
    • Related Report
      2012 Final Research Report
  • [Presentation] Na+駆動型キメラべん毛モーターにおける固定子PomA融合タンデムダイマーの機能解析2011

    • Author(s)
      蔡栄淑, 曽和義幸.
    • Organizer
      日本生物物理学会 第49回生物物理学会年会
    • Place of Presentation
      兵庫県立大学 (兵庫県)
    • Related Report
      2012 Final Research Report
  • [Presentation] 高圧力下にあるバクテリア運動能の顕微解析2011

    • Author(s)
      西山雅祥,曽和義幸.
    • Organizer
      日本生物物理学会 第49回生物物理学会年会
    • Place of Presentation
      兵庫県立大学 (兵庫県)
    • Related Report
      2012 Final Research Report
  • [Presentation] Three-dimensinal tracking of bacterial flagellar rotation2011

    • Author(s)
      曽和義幸
    • Organizer
      日本生物物理学会
    • Place of Presentation
      兵庫県立大学 (兵庫)
    • Related Report
      2011 Research-status Report
  • [Presentation] Dynamic conformational changes of flagellar filament observed by high-pressure microscopy

    • Author(s)
      Nishiyama, M.
    • Organizer
      57th Annual Meeting of Biophysical Society
    • Place of Presentation
      Pennsylvania (アメリカ)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Bacterial motility measured by a high-pressure microscope

    • Author(s)
      Nishiyama, M.
    • Organizer
      7th International Conference on High Pressure Bioscience and Biotechnology
    • Place of Presentation
      ピアザ淡海 (滋賀県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Exploring the mechanism underlying sensing of the repellent Ni2+ by the aspartate chemoreceptor Tar of Escherichia coli.

    • Author(s)
      Inui, T.
    • Organizer
      第50回生物物理学会年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Control of bacterial flagellar rotation via crosstalk from a non-cognate histidine kinase to the response regulator CheY.

    • Author(s)
      Umemura, T.
    • Organizer
      第50回生物物理学会年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Intracellular dynamics of xenobiotic efflux proteins in Escherichia coli.

    • Author(s)
      Yamamoto, K.
    • Organizer
      第50回生物物理学会年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Imaging of fluorescently-tagged motor components of bacterial flagella

    • Author(s)
      Sowa, Y.
    • Organizer
      第50回生物物理学会年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Rotational measurement of Na+-driven chimeric flagellar motor with tandem PomA.

    • Author(s)
      Che, Y-S
    • Organizer
      第50回生物物理学会年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Dynamic conformational changes of flagellar filament observed by high-pressure microscopy

    • Author(s)
      Nishiyama, M.
    • Organizer
      第50回生物物理学会年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Sodium dynamics of the bacterial flagellar motor.

    • Author(s)
      Lo, C-J.
    • Organizer
      第50回生物物理学会年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] A new setup for tracking of bacterial flagellar rotation in 3-dimensionally

    • Author(s)
      曽和義幸
    • Organizer
      2012 Gordon Research Conference on Sensory Transduction in Microorganisms
    • Place of Presentation
      Ventura Beach Marriot (USA)
    • Related Report
      2011 Research-status Report
  • [Book] ComprehensiveBiphysics AcademicPress2012

    • Author(s)
      YoshiyukiSowa&RichardM.Berry
    • Publisher
      TheRotaryBacterialFlagellarMotor
    • Related Report
      2012 Final Research Report
  • [Book] "The Rotary Bacterial Flagellar Motor." in Comprehensive Biphysics (Edward Egelman ed.)2012

    • Author(s)
      Sowa, Y.
    • Publisher
      Academic Press
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://sowalab.ws.hosei.ac.jp

    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 高速かつ高効率なゲノム改変法2012

    • Inventor(s)
      梅野太輔,冨永将大,田代洋平,川 岸郁朗,曽和義幸,稲葉岳彦,蔡栄淑
    • Industrial Property Rights Holder
      国立大学法人 千葉大学, 学校法人 法政大学
    • Industrial Property Number
      2012-113083
    • Filing Date
      2012-05-17
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 高速かつ高効率なゲノム改変法2011

    • Inventor(s)
      梅野,冨永,田代,川岸,曽和,稲葉,蔡
    • Industrial Property Rights Holder
      千葉大学,法政大学
    • Industrial Property Number
      2011-110420
    • Filing Date
      2011
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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