Development of real-time secretion analysis of immune single cells
| Project/Area Number |
23770189
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SHIRASAKI Yoshitaka 独立行政法人理化学研究所, 免疫ゲノミクス研究グループ, 研究員 (70469948)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 単一細胞解析 / イムノアッセイ / 分泌測定 / マイクロウェル / CYTOP / 単一細胞測定 / 免疫システム |
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| Research Abstract |
We developed a platform for real-time secretion analysis at the single cell resolution. The platform enabled to monitor secretion dynamics of thousand of single cells with 30-min time resolution for over 8 hours. We also demonstrated multi-parametric measurement of secretion dynamics and cellular states with 1-min time resolution. We applied our platform to inflammatory responses of immune cells and observed intercellular and intracellular heterogeneity of cytokine secretion.
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Report
(3 results)
Research Products
(17 results)
