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A study on ion-channel gating by single molecule measurement and manipulation.

Research Project

Project/Area Number 23770190
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

HIRANO Minako  独立行政法人理化学研究所, 細胞動態計測研究グループ, 客員研究員 (80585167)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords分子計測 / イオンチャネル / 蛍光色素 / 1分子計測 / イメージング
Research Abstract

Using environment-sensitive fluorophore, conformational changes of a single ion channel were clearly detected. And, to clarify the relationship between conformational and functional changes of an ion channel, I improved an apparatus to measure these changes simultaneously at the single channel level. Using this apparatus, functional changes were easily detected and fluorescence images of a single ion channel were stably obtained.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (19 results)

All 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (13 results) Remarks (1 results)

  • [Journal Article] Uncovering the Protein Translocon at the Chloroplast Inner Envelope Membrane2013

    • Author(s)
      Shingo Kikuchi
    • Journal Title

      SCIENCE

      Volume: 339 Issue: 6119 Pages: 571-574

    • DOI

      10.1126/science.1229262

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 「ちから」でイオンチャネルの機能を制御する2012

    • Author(s)
      井出徹、平野美奈子、奥野大地
    • Journal Title

      生物物理学会誌

      Volume: 52(6) Pages: 289-290

    • NAID

      10031126103

    • URL

      https://www.jstage.jst.go.jp/article/biophys/52/6/52_289/_pdf

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] The role of the KcsA channel cytoplasimic domain in pH-dependent gating2011

    • Author(s)
      M.Hirano, Y.Onishi, T.Yanagida, T.Ide
    • Journal Title

      Biophysical Journal

      Volume: 101(9) Issue: 9 Pages: 2157-2162

    • DOI

      10.1016/j.bpj.2011.09.024

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Direct Manipulation of a Single Potassium Channel Gate with an Atomic Force Microscope Probe2011

    • Author(s)
      M.Kitta, T.Ide, M.Hirano, H.Tanaka, T.Yanagida, T.Kawai
    • Journal Title

      Small

      Volume: 7(16) Issue: 16 Pages: 2379-2383

    • DOI

      10.1002/smll.201002337

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Role of the KcsA channel cytoplasmic domain in pH-dependent gating2011

    • Author(s)
      M. Hirano, Y. Onishi, T. Yanagida, T. Ide
    • Journal Title

      Biophysical Journal

      Volume: 101 Pages: 2157-2162

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] The KcsA channel cytoplasmic domain effects on the inactivation gating2013

    • Author(s)
      M. Hirano, Y. Onishi, D. Okuno, T. Ide
    • Organizer
      Biophysical Society 57th Annual Meeting
    • Place of Presentation
      フィラデルフィア(アメリカ合衆国)
    • Related Report
      2012 Final Research Report
  • [Presentation] The KcsA channel cytoplasmic domain effects on the inactivation gating2013

    • Author(s)
      M. Hirano, Y. Onishi, D. Okuno, T. Ide
    • Organizer
      Biophysical Society 57th Annual Meeting
    • Place of Presentation
      フィラデルフィア(アメリカ合衆国)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Single molecule imaging of bio-molecules2012

    • Author(s)
      T. Ide, M. Hirano, D. Okuno
    • Organizer
      The 14th Takayanagi Kenjiro Memorial Symposium
    • Place of Presentation
      静岡
    • Year and Date
      2012-11-28
    • Related Report
      2012 Final Research Report
  • [Presentation] Reconstitution of ion channel into lipid bilayer using glass needle2012

    • Author(s)
      D. Okuno, M. Hirano, Y. Onishi, T. Yanagida, T. Ide
    • Organizer
      第50回 日本生物物理学会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Automated drug screening system for ion channel proteins using artificial cell membranes2012

    • Author(s)
      R. Kawano, Y. Tuji, K. Kimiya, T. Osaki, M. Hirano, T. Ide, N. Miki, S.Takeuchi
    • Organizer
      第50回 日本生物物理学会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] The KcsA channel cytoplasmic domain effects on the inactivation gating2012

    • Author(s)
      M. Hirano, Y. Onishi, D. Okuno, T. Ide
    • Organizer
      第50回 日本生物物理学会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-23
    • Related Report
      2012 Final Research Report
  • [Presentation] The KcsA channel cytoplasmic domain effects on the inactivation gating2012

    • Author(s)
      M. Hirano, Y. Onishi, D. Okuno, T. Ide
    • Organizer
      Molecular Science of Fluctuations toward Biological Functions, The 6th International Symposium
    • Place of Presentation
      京都
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] The KcsA channel cytoplasmic domain effects on the inactivation gating2012

    • Author(s)
      M. Hirano, Y. Onishi, D. Okuno, T. Ide
    • Organizer
      第50回 日本生物物理学会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report
  • [Presentation] Mechanism for opening and closing by the KcsA channel2011

    • Author(s)
      M. Hirano, Y. Onishi, T. Yanagida, T. Ide
    • Organizer
      第49回 日本生物物理学会
    • Place of Presentation
      兵庫
    • Year and Date
      2011-09-18
    • Related Report
      2012 Final Research Report
  • [Presentation] The KcsA channel opening and closing mechanism2011

    • Author(s)
      M. Hirano
    • Organizer
      QBiC Retreat 2011: How to achieve "Whole Cell Modeling"
    • Place of Presentation
      兵庫
    • Year and Date
      2011-09-10
    • Related Report
      2012 Final Research Report
  • [Presentation] 蛍光色素を利用したイオンチャネルの開閉機構の可視化2011

    • Author(s)
      平野美奈子
    • Organizer
      新学術領域研究「細胞機能と分子活性の多次元蛍光生体イメージング」第1回研究代表者会議
    • Place of Presentation
      北海道大学
    • Related Report
      2011 Research-status Report
  • [Presentation] Mechanism for opening and closing by the KcsA channel2011

    • Author(s)
      Minako Hirano
    • Organizer
      QBiC Retreat 2011: How to achieve "Whole Cell Modeling"
    • Place of Presentation
      ユニトピアささやま
    • Related Report
      2011 Research-status Report
  • [Presentation] Mechanism for opening and closing by the KcsA channel2011

    • Author(s)
      平野美奈子
    • Organizer
      第49回 日本生物物理学会
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.gpi.ac.jp/research/teacher/professor-06.html

    • Related Report
      2012 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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