Analysis of mechanisms of translational control using transgenes and visualization of the site and timing of translation

• Project/Area Number: 23770196
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Hokkaido University
• Principal Investigator: KOTANI Tomoya 北海道大学, 大学院・理学研究院, 准教授 (70419852)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 翻訳 / mRNA局在 / シス因子 / コード領域 / 卵母細胞 / 卵成熟 / サイクリンB1 / 局在 / 翻訳時期

Research Abstract

Localization and translational control of cytoplasmic mRNAs play key roles in the regulation of site and timing of gene expression. We analyzed molecular mechanisms by which localization and translational control of cyclin B1 mRNA deposited in oocytes are regulated using transgenes and real-time imaging of the site and timing of translation. In this study, we revealed that a cis-acting element consisting of 9 nucleotides sequences in the coding region is necessary for the regulation of both localization and translational control of the mRNA. Our results suggest a functional link between mRNA localization and translational control.

Research Products

• [Journal Article] A cis-acting element in the coding region of cyclin B1 mRNA couples subcellular localization to translational timing (2013)
  • Author(s): Yasuda, K., Kotani, T., Yamashita, M.
  • Journal Title: Developmental Biology Volume: 印刷中
  • NAID: 120005348193
  • Peer Reviewed
• [Journal Article] Possible involvement of Nemo-like kinase 1 in Xenopus oocyte maturation as a kinase responsible for Pumilio1, Pumilio2, and CPEB phosphorylation (2011)
  • Author(s): Ota, R., Kotani, T., Masakane, Y.
  • Journal Title: Biochemistry Volume: 50 Issue: 25 Pages: 5648-5659
  • DOI: 10.1021/bi2002696
  • Peer Reviewed
• [Journal Article] Biochemical characterization of pumilio1 and pumilio2 in Xenopus oocytes (2011)
  • Author(s): Ota, R., Kotani, T., Yamashita, M.
  • Journal Title: Journal of Biological Chemistry Volume: 286 Issue: 4 Pages: 2853
  • DOI: 10.1074/jbc.m110.155523
  • URL: https://pure.teikyo.jp/en/publications/f73a4264-0247-4192-a8c7-540eafa68a33
  • Peer Reviewed
• [Journal Article] A cis-acting element in the coding region of cyclin B1 mRNA couples subcellular localization to translational timing
  • Author(s): Kyota Yasuda, Tomoya Kotani,Masakane Yamashita
  • Journal Title: Developmental Biology
  • NAID: 120005348193
  • Peer Reviewed
• [Presentation] 卵成熟過程でのゼブラフィッシュcyclin B1 mRNA翻訳に関する新規シス配列の同定 (2012)
  • Author(s): 安田恭太、小谷友也、山下正兼
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学
  • Year and Date: 2012-09-13
• [Presentation] Translational control of cyclin B1 mRNA by RNA granule formation in vertebrate oocytes (2012)
  • Author(s): Tomoya Kotani, Kyota Yasuda, Ryoma Ota, Masakane Yamashita
  • Organizer: Joint meeting of JSDB 45th and JSCB 64th
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2012-05-29
• [Presentation] Transgeniczebrafish reveals a novel cis-acting element responsible for the spatio-temporally regulated translational activation of cyclin B1 mRNA in oocytes (2012)
  • Author(s): Kyota Yasuda, Tomoya Kotani, Masakane Yamashita
  • Organizer: Joint meeting of JSDB 45th and JSCB 64th
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2012-05-28
• [Presentation] Translational control of cyclin B1 mRNA by RNA granule formation in vertebrate oocytes (2012)
  • Author(s): Kotani, T., Yasuda, K., Ota, R., Yamashita, M.
  • Organizer: Joint meeting of JSDB 45th and JSCB 64th
  • Place of Presentation: 神戸コンベンションセンター（神戸市）
• [Presentation] Transgenic zebrafish reveals a novel cis-acting element responsible for the spatio-temporally regulated translational activation of cyclin B1 mRNA in oocytes (2012)
  • Author(s): Yasuda, K., Kotani, T., Yamashita, M.
  • Organizer: Joint meeting of JSDB 45th and JSCB 64th
  • Place of Presentation: 神戸コンベンションセンター（神戸市）
• [Presentation] Possible involvement of Nemo-like Kinase 1 in Xenopus oocyte maturation as a kinase that catalyzes Pum1, Pum2 and CPEB phosphorylation required for the translation of dormant mRNAs in oocytes (2011)
  • Author(s): Ryoma Ota, Tomoya Kotani, MasakaneYamashita
  • Organizer: 44th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2011-05-20
• [Presentation] 卵成熟過程でのゼブラフィッシュcyclin B1 mRNA翻訳制御に関する新規シス配列の同定
  • Author(s): 安田恭太、小谷友也、山下正兼
  • Organizer: 日本動物学会 第83回大会
  • Place of Presentation: 大阪大学（豊中市）