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Mechanism of translation arrest and degradation of aberrant products in mRNA quality control systems

Research Project

Project/Area Number 23770198
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Molecular biology
Research InstitutionTohoku University

Principal Investigator

KUROHA Kazushige  東北大学, 大学院・薬学研究科, 研究支援者 (50580015)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
KeywordsmRNA 品質管理 / mRNA 分解 / タンパク質分解 / mRNA品質管理 / mRNA分解 / 翻訳アレスト / 品質管理機構
Research Abstract

We have analyzed two major of aberrant mRNAs, stop codon-less mRNA and nonsense-containing mRNA, to reveal the mechanism of the aberrant translation recognition and stimulation of aberrant products degradation. We found that mechanism of stop codon-less mRNA degradation by Dom34:Hbs1 complex. We also found that the mis-folded conformation of PTC products is important for leading to its degradation by Upf factors.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (13 results)

All 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (8 results) Remarks (2 results)

  • [Journal Article] Roles of dom34 : hbs1 in nonstop protein clearance from translocators for normal organelle protein influx.2012

    • Author(s)
      Toshiaki Izawa, Tatsuhisa Tsuboi, Kazushige Kuroha, et al.
    • Journal Title

      Cell Reports

      Volume: 2 Issue: 3 Pages: 447-453

    • DOI

      10.1016/j.celrep.2012.08.010

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dom34:Hbs1 Plays a General Role in Quality-Control Systems by Dissociation of a Stalled Ribosome at the 3' End of Aberrant mRNA2012

    • Author(s)
      Tatsuhisa Tsuboi, et al
    • Journal Title

      Molecular Cell

      Volume: 46 Issue: 4 Pages: 1-12

    • DOI

      10.1016/j.molcel.2012.03.013

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Roles of dom34:hbs1 in nonstop protein clearance from translocators for normal organelle protein influx.2012

    • Author(s)
      Izawa T, Tsuboi T, Kuroha K, Inada T, Nishikawa S, Endo T.
    • Journal Title

      Cell Reports

      Volume: 2 Pages: 447-453

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] NMD(ナンセンス変異依存mRNA分解)における異常タンパク質分解機構の解析2012

    • Author(s)
      黒羽一誠、稲田利文
    • Organizer
      RNAフロンティアミーティング
    • Place of Presentation
      メルパルク熊本
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Quality control systems for aberrant products derived from mRNAs containing a specific premature termination codon in yeast2012

    • Author(s)
      黒羽一誠、稲田利文
    • Organizer
      Translational Control
    • Place of Presentation
      Coldspring Harbor、アメリカ
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] NMD(ナンセンス変異依存mRNA分解)における異常タンパク質分解機構の解析2012

    • Author(s)
      黒羽一誠、稲田利文
    • Organizer
      RNA meeting Tohoku
    • Place of Presentation
      東北大学萩ホール
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] NMD(ナンセンス依存mRNA分解)における異常タンパク質分解機構の解析2012

    • Author(s)
      黒羽一誠、稲田利文
    • Organizer
      Ribosome meeting
    • Place of Presentation
      広島大学生物生産学部
    • Related Report
      2012 Final Research Report
  • [Presentation] NMD(ナンセンス依存mRNA)における異常タンパク質分解機構の解析2012

    • Author(s)
      黒羽一誠、稲田利文
    • Organizer
      Ribosome meeting
    • Place of Presentation
      広島大学生物生産学部
    • Related Report
      2011 Research-status Report
  • [Presentation] NMD(ナンセンス変異依存mRNA分解)における異常タンパク質分解機構の解析2011

    • Author(s)
      黒羽一誠、稲田利文
    • Organizer
      日本生化学会東北支部会
    • Place of Presentation
      東北大学さくらホール
    • Year and Date
      2011-07-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Quality control systems for aberrant products derived from mRNAs containing a specific premature termination codon in yeast2011

    • Author(s)
      黒羽一誠、稲田利文
    • Organizer
      RNA2011
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] NMD(ナンセンス変異依存mRN分解)における異常タンパク質分解機構の解析2011

    • Author(s)
      黒羽一誠、稲田利文
    • Organizer
      日本生化学会 東北支部会
    • Place of Presentation
      東北大学さくらホール
    • Related Report
      2011 Research-status Report
  • [Remarks] 東北大学薬学研究科遺伝子制御薬学分野

    • URL

      http://www.pharm.tohoku.ac.jp/?idenshi/inada_lab_HP/HOME(Japanese).html

    • Related Report
      2012 Final Research Report
  • [Remarks] 東北大学薬学研究科遺伝子制御薬学分野

    • URL

      http://www.pharm.tohoku.ac.jp/~idenshi/inada_lab_HP/HOME(Japanese).html

    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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