• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanism of protein synthesis in mammalian mitochondria

Research Project

Project/Area Number 23770202
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Molecular biology
Research InstitutionThe University of Tokyo

Principal Investigator

TOMITA Nono (TAKEUCHI Nono)  東京大学, 大学院・新領域創成科学研究科, 准教授 (80323450)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords蛋白質合成 / ミトコンドリア / タンパク質合成
Research Abstract

We have obtained following results on the mechanism of protein synthesis in mammalian mitochondrial. (1) The role of GTP-hydrolysis during ribosome recycling process has been clarified. (2) The sturucture of 55S ribosome has been determined at 10 A resolution by cryoEM reconstitution. The E-site tRNA on 55S ribosome has been visualized. (3) The ribosome binding properties of the mitochondrial ribosome biogenesis factor (ObgH1, Mtg1) have been clarified.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (14 results)

All 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] Human G-proteins, ObgH1 and Mtg1, associate with the large mitochondrial ribosome subunit and are involved in translation and assembly of respiratory complexes2013

    • Author(s)
      Kotani, T.
    • Journal Title

      Nucleic acids research

      Volume: 2013 Issue: 6 Pages: 3713-3722

    • DOI

      10.1093/nar/gkt079

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Possible steps ofcomplete disassembly of post-termination complex by yeast eEF3 deduced from inhibition by translocation inhibitors.2012

    • Author(s)
      Kurata S., Shen B., Liu JO., Takeuchi N., Kaji A. and Kaji H.
    • Journal Title

      Nucleic Acids Res.

      Volume: 41(1) Issue: 1 Pages: 264-76

    • DOI

      10.1093/nar/gks958

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] RsfA (YbeB) Proteins Are Conserved Ribosomal Silencing Factors.2012

    • Author(s)
      Hauser R.
    • Journal Title

      PLoS Genet.

      Volume: 8(7) Issue: 7 Pages: e1002815-e1002815

    • DOI

      10.1371/journal.pgen.1002815

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Taurine-containing uridine modifications in tRNA anticodons are required to decipher non-universal genetic codes in ascidian mitochondria2011

    • Author(s)
      Suzuki T (第1著者), 他8名
    • Journal Title

      J. Biol. Chem

      Volume: 286 Issue: 41 Pages: 35494

    • DOI

      10.1074/jbc.m111.279810

    • URL

      https://pure.teikyo.jp/en/publications/70d6d0f7-88b7-4afe-a2b1-a2ea848a121b

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Taurine-containing uridine modifications in tRNA anticodons are required to decipher non-universal genetic codes in ascidian mitochondria.2011

    • Author(s)
      Suzuki T., Miyauchi K., Suzuki T., Yokobori S., Shigi N., Kondow A., Takeuchi N., Yamagishi A., Watanabe K.
    • Journal Title

      J. Biol. Chem.

      Volume: 286 Pages: 35494-8

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] N-terminal extension of mammalian mitochondrial2012

    • Author(s)
      Nono Tomita-Takeuchi
    • Organizer
      RRF alters the role of GTP-hydrolysis by EF-G in ribosome recycling Complex life of mRNA
    • Place of Presentation
      EMBL, Heidelberg, Germany
    • Year and Date
      2012-10-09
    • Related Report
      2012 Final Research Report
  • [Presentation] Mechanism of ribosome recycling in mammalian mitochondrialprotein synthesis2012

    • Author(s)
      入江遼平
    • Organizer
      日本RNA学会年会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2012-07-19
    • Related Report
      2012 Final Research Report
  • [Presentation] Functional analysis of Mtg1 in mammalian mitochondrial ribosomebiogenesis2012

    • Author(s)
      小谷哲也
    • Organizer
      日本RNA学会年会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2012-07-19
    • Related Report
      2012 Final Research Report
  • [Presentation] N-terminal extension of mammalian mitochondrial RRF alters the role of GTP-hydrolysis by EF-G in ribosome recycling2012

    • Author(s)
      Nono Tomita-Takeuchi
    • Organizer
      Complex life of mRNA
    • Place of Presentation
      EMBL, Heidelberg, Germany
    • Related Report
      2012 Annual Research Report
  • [Presentation] Protein Synthesis System of Mammalian Mitochondria2011

    • Author(s)
      Nono Takeuchi
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-15
    • Related Report
      2012 Final Research Report
  • [Presentation] Protein Synthesis System of Mammalian Mitochondria2011

    • Author(s)
      Nono Takeuchi
    • Organizer
      日本分子生物学会(招待講演)
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Functional analysis of Mtg1 in mammalian mitochondrial ribosome biogenesis

    • Author(s)
      小谷哲也
    • Organizer
      日本RNA学会年会
    • Place of Presentation
      東北大学(仙台)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Mechanism of ribosome recycling in mammalian mitochondrial protein synthesis

    • Author(s)
      入江遼平
    • Organizer
      日本RNA学会年会
    • Place of Presentation
      東北大学(仙台)
    • Related Report
      2012 Annual Research Report
  • [Remarks] なし

    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi