Mechanism of protein synthesis in mammalian mitochondria

• Project/Area Number: 23770202
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: The University of Tokyo
• Principal Investigator: TOMITA Nono (TAKEUCHI Nono) 東京大学, 大学院・新領域創成科学研究科, 准教授 (80323450)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 蛋白質合成 / ミトコンドリア / タンパク質合成

Research Abstract

We have obtained following results on the mechanism of protein synthesis in mammalian mitochondrial. (1) The role of GTP-hydrolysis during ribosome recycling process has been clarified. (2) The sturucture of 55S ribosome has been determined at 10 A resolution by cryoEM reconstitution. The E-site tRNA on 55S ribosome has been visualized. (3) The ribosome binding properties of the mitochondrial ribosome biogenesis factor (ObgH1, Mtg1) have been clarified.

Research Products

• [Journal Article] Human G-proteins, ObgH1 and Mtg1, associate with the large mitochondrial ribosome subunit and are involved in translation and assembly of respiratory complexes (2013)
  • Author(s): Kotani, T.
  • Journal Title: Nucleic acids research Volume: 2013 Issue: 6 Pages: 3713-3722
  • DOI: 10.1093/nar/gkt079
  • Peer Reviewed
• [Journal Article] Possible steps ofcomplete disassembly of post-termination complex by yeast eEF3 deduced from inhibition by translocation inhibitors. (2012)
  • Author(s): Kurata S., Shen B., Liu JO., Takeuchi N., Kaji A. and Kaji H.
  • Journal Title: Nucleic Acids Res. Volume: 41(1) Issue: 1 Pages: 264-76
  • DOI: 10.1093/nar/gks958
  • Peer Reviewed
• [Journal Article] RsfA (YbeB) Proteins Are Conserved Ribosomal Silencing Factors. (2012)
  • Author(s): Hauser R.
  • Journal Title: PLoS Genet. Volume: 8(7) Issue: 7 Pages: e1002815-e1002815
  • DOI: 10.1371/journal.pgen.1002815
  • Peer Reviewed
• [Journal Article] Taurine-containing uridine modifications in tRNA anticodons are required to decipher non-universal genetic codes in ascidian mitochondria (2011)
  • Author(s): Suzuki T (第1著者), 他8名
  • Journal Title: J. Biol. Chem Volume: 286 Issue: 41 Pages: 35494-35498
  • DOI: 10.1074/jbc.m111.279810
  • Peer Reviewed
• [Journal Article] Taurine-containing uridine modifications in tRNA anticodons are required to decipher non-universal genetic codes in ascidian mitochondria. (2011)
  • Author(s): Suzuki T., Miyauchi K., Suzuki T., Yokobori S., Shigi N., Kondow A., Takeuchi N., Yamagishi A., Watanabe K.
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 35494-8
  • Peer Reviewed
• [Presentation] N-terminal extension of mammalian mitochondrial (2012)
  • Author(s): Nono Tomita-Takeuchi
  • Organizer: RRF alters the role of GTP-hydrolysis by EF-G in ribosome recycling Complex life of mRNA
  • Place of Presentation: EMBL, Heidelberg, Germany
  • Year and Date: 2012-10-09
• [Presentation] Mechanism of ribosome recycling in mammalian mitochondrialprotein synthesis (2012)
  • Author(s): 入江遼平
  • Organizer: 日本RNA学会年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2012-07-19
• [Presentation] Functional analysis of Mtg1 in mammalian mitochondrial ribosomebiogenesis (2012)
  • Author(s): 小谷哲也
  • Organizer: 日本RNA学会年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2012-07-19
• [Presentation] N-terminal extension of mammalian mitochondrial RRF alters the role of GTP-hydrolysis by EF-G in ribosome recycling (2012)
  • Author(s): Nono Tomita-Takeuchi
  • Organizer: Complex life of mRNA
  • Place of Presentation: EMBL, Heidelberg, Germany
• [Presentation] Protein Synthesis System of Mammalian Mitochondria (2011)
  • Author(s): Nono Takeuchi
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-15
• [Presentation] Protein Synthesis System of Mammalian Mitochondria (2011)
  • Author(s): Nono Takeuchi
  • Organizer: 日本分子生物学会(招待講演)
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] Functional analysis of Mtg1 in mammalian mitochondrial ribosome biogenesis
  • Author(s): 小谷哲也
  • Organizer: 日本RNA学会年会
  • Place of Presentation: 東北大学（仙台）
• [Presentation] Mechanism of ribosome recycling in mammalian mitochondrial protein synthesis
  • Author(s): 入江遼平
  • Organizer: 日本RNA学会年会
  • Place of Presentation: 東北大学（仙台）
• [Remarks] なし